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Pseudomonas capable of oxidizing antimony and arsenic and application of pseudomonas

A technology of antimony arsenic oxidizing pseudomonas and pseudomonas, which is applied in the field of microorganisms, can solve the problems of less research, and achieve the effects of simple culture conditions, easy storage, and good development and application potential

Active Publication Date: 2020-09-18
HUNAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The screening and identification of efficient antimony oxidizing microorganisms is one of the cornerstones of microbial antimony removal technology, but there are relatively few studies in this area

Method used

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  • Pseudomonas capable of oxidizing antimony and arsenic and application of pseudomonas
  • Pseudomonas capable of oxidizing antimony and arsenic and application of pseudomonas
  • Pseudomonas capable of oxidizing antimony and arsenic and application of pseudomonas

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Example 1 Isolation and screening of highly efficient antimony arsenic oxidative pseudomonas DHHN0901

[0032] The bacterial strain of the present invention belongs to the genus Pseudomonas, Pseudomonas sp., and the strain name is DHHN0901. The strain is isolated and screened from the flower bed loess near the slag heap of the Xikuangshan Smelter in Lengshuijiang City, Hunan Province, and specifically obtained by plate marking screening. The screening method belongs to the conventional experimental method for strain isolation and screening in the field.

[0033] The specific method and process are described as follows: Weigh 10g of soil sample and add it into a 250ml sterilized conical flask; soak in 90ml of sterile water, shake it on a shaker at a temperature of 30°C for 30min, and let it stand for 30s; use serial dilution to coat Bacteria were isolated by the method, and after single colonies were selected, they were streaked and purified on the solid medium, and the ...

Embodiment 2

[0037] Example 2 Antimony resistance performance test of highly efficient antimony-arsenic oxidizing bacteria DHHN0901 (solid medium)

[0038] Lines were drawn on the CDM solid medium with different antimony concentrations, and cultured in a constant temperature incubator at 30°C to measure the resistance to antimony. Three replicate groups were set up for each group. The high-efficiency antimony-oxidizing bacteria DHHN0901 can grow normally on the solid medium containing antimony concentration of 0-8000mg / L, and the antimony concentration is 9000mg / L. The growth is extremely slow and hardly grows.

[0039]Compared with other research reports, Pseudomonas DHHN0901 has higher resistance to heavy metal antimony than other reported microorganisms. The strain lived in heavy metal polluted areas for a long time, and its strong resistance to heavy metal antimony may be related to its complex living environment.

Embodiment 3

[0040] Example 3 Molecular, Physiological and Biochemical Identification of Efficient Antimony Arsenic Pseudomonas Oxans DHHN0901

[0041] 1. In this example, the Pseudomonas (Pseudomonas) DHHN0901 of the present invention is tested and analyzed by 16S rRNA sequence.

[0042] 16S rRNA related identification method:

[0043] Use the bacterial universal primers to carry out PCR identification on the screened bacterial strains: extract the template DNA according to the operating instructions of the bacterial DNA extraction kit. Using the upstream primer M13F (TGTAAAACGACGGCCAGT) and the downstream primer M13R (CAGGAAACAGCTAT GACC) of the 16S rRNA conserved sequence, the 16S rRNA gene fragment of Pseudomonas (Pseudomonas) DHHN0901 was amplified by PCR, cloned and sequenced.

[0044] After sequencing, a fragment of 1485bp was obtained, and the accession number in GenBank was MN720563. After sequencing, it was found that the bacterium belonged to Pseudomonas, and the sequencing res...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to pseudomonas capable of oxidizing antimony and arsenic and application of the pseudomonas. A pseudomonas strain is separated and screened from antimony-polluted soil in a stannary in Lengshuijiang City, Hunan Province and has efficient antimony and / or efficient arsenic oxidation performance; bacteria can still survive in a solution and soil with high antimony content and / or arsenic content, has relatively good antimony tolerance and arsenic tolerance and has excellent antimony arsenic oxidability and / orarsenic oxidability after being tested, so that an important foundation is laid for development of a technique for removing antimony and / or arsenic through adsorption of the microorganisms. Meanwhile,the strain combined with Fe<3+> has a good improvement effect, the oxidizing efficiency of the strain can be improved, and the strain has good development and application potentialities.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of antimonas arsenic oxidizing pseudomonas and its application. Background technique [0002] Antimony (Sb) has the characteristics of chronic toxicity, carcinogenicity, and global migration, and is a global pollutant and a priority pollutant. Antimony (Sb) and its compounds can enter the human body through food chains and water, which not only affect the function of certain enzymes, but also cause diseases in the liver and cardiovascular system. The content of antimony in the medium is strictly limited (<5 μg / L), and the toxicity of trivalent antimony is ten times that of pentavalent antimony. Therefore, the oxidation of Sb(III) to Sb(V) is beneficial to reduce its environmental hazards and risks. At home and abroad, some research has been done on how to oxidize Sb(Ⅲ) to Sb(Ⅴ), such as adding chemical oxidants and other treatment technologies. Al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/38C02F101/10C02F101/20
CPCC02F3/34B09C1/10C02F2101/103C02F2101/20C12R2001/38C12N1/205
Inventor 邓仁健黄中杰周赛军赵家欣李小坚张凯昕任伯帜
Owner HUNAN UNIV OF SCI & TECH
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