Oleophylic fertilizer for bioremediation of oil polluted environment and method for preparing oleophylic fertilizer
A technology of bioremediation and oil pollution, which is applied in the field of environmental treatment of oil pollution, can solve problems such as inapplicable bioremediation, and achieve the effect of improving bioavailability, eliminating oil film, and promoting bioremediation
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Embodiment 1
[0032] Embodiment 1 takes urea as the preparation of the lipophilic fertilizer of nitrogen source
[0033] Prepare a mixture of soybean lecithin and co-emulsifier according to the mass ratio (Km value) of 1:2, heat at 75°C for 10 minutes, vortex and mix for 2 minutes, and after ultrasonic dissolution for 10 minutes, weigh oleic acid and the co-emulsifier according to the mass ratio of 3:7. 2 g of the mixture was vortexed for 15 min to mix well. Add 600 μl of saturated urea solution, vortex and mix for 10 minutes, and then let it stand for about 30 minutes to balance and form a water-in-oil microemulsion. Sealed and stored to get the product.
Embodiment 2
[0034] Example 2 Adding lipophilic fertilizers to oil-polluted seawater to observe the state of the oil with a microscope
[0035] Get 50ml seawater sample in the Erlenmeyer flask of 150ml, add 0.5ml diesel oil respectively, one bottle adds the lipophilic fertilizer 100 μ l prepared in embodiment 1, another bottle does not add, after shaking at 100rpm rotating speed simultaneously 12h, get sample in Observed under a microscope, under the magnification of 100 times, the state of diesel oil is as follows: figure 1 As shown, the oleophilic fertilizer makes the oil film form small oil droplets and aggregate, and at the same time attach to the oil.
Embodiment 3
[0036] The promotion of embodiment 3 lipophilic fertilizers to bacterial strain degradation diesel oil
[0037] Four strains of diesel-oil-degrading bacteria isolated from oil-polluted seawater were cultured overnight in LB medium, then the bacteria were collected and inoculated into 150ml Erlenmeyer flasks containing 50ml of seawater samples, and 0.5ml of diesel oil was added to each bottle. 100 μl of the lipophilic fertilizer prepared in Example 1 was not added to the other bottle. After culturing in a shaker at 30° C. and 180 rpm for 4 days, the degradation rate of diesel oil was measured. The degradation rate of diesel oil was only 8% without adding lipophilic fertilizer, but it reached 64% under the condition of adding lipophilic fertilizer.
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