Biological type bone repairing material and preparation method thereof

A repair material and bone repair technology, applied in the field of biological repair, can solve the problems of large differences in mechanical properties of human bones, etc., and achieve the effects of less chemical residues, high biological safety, and low toxicity

Inactive Publication Date: 2019-03-29
SUMMIT GD BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it is true that heterogeneous bone repair materials can be prepared by using the above-mentioned traditional preparation process, the mechanical properties of these heterogeneous bone repair materials are quite different from those of human bone.

Method used

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  • Biological type bone repairing material and preparation method thereof

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Effect test

Embodiment 1

[0033] This embodiment provides a heterogeneous decellularized bone repair material and a preparation method thereof. This preparation method comprises the steps:

[0034] (1) Cut the bovine cancellous bone into 5mm granular shapes, wash it repeatedly with a high-pressure water gun until the color of the bone tissue is white to yellowish, and there is no visible bone marrow tissue, and dry it in a drying oven for 12 hours.

[0035] (2) Soak the cancellous bone with a mass fraction of 65% hydrogen peroxide at 8° C. for 24 hours after the drying in step (1), and repeatedly clean the cancellous bone after soaking with deionized water, and place it in a drying oven Dry for 8 hours.

[0036] (3) Soak the cancellous bone dried in step (2) with 0.6M sodium hydroxide at 5°C for 12 hours, wash the soaked cancellous bone repeatedly with distilled water, and dry it in a drying oven for 8 hours .

[0037] (4) Place the dried cancellous bone in step (3) together with the mixed solution ...

Embodiment 2

[0041] This embodiment provides a heterogeneous decellularized bone repair material and a preparation method thereof. The preparation method comprises the steps of:

[0042] (1) Cut the bovine cancellous bone into 5mm granular shapes, wash it repeatedly with a high-pressure water gun until the color of the bone tissue is white to yellowish, and there is no visible bone marrow tissue, and dry it in a drying oven for 12 hours.

[0043] (2) Soak the cancellous bone with 30% hydrogen peroxide with a mass fraction of 30% after step (1) and soak it for 12 hours at 3°C. The soaked cancellous bone is washed repeatedly with deionized water and placed in a drying oven. Dry for 8 hours.

[0044] (3) Soak the cancellous bone dried in step (2) with 4M sodium hydroxide at 70°C for 48 hours, wash the soaked cancellous bone repeatedly with distilled water, and dry it in a drying oven for 8 hours.

[0045] (4) Place the dried cancellous bone in step (3) together with the mixed solution of et...

Embodiment 3

[0049] This embodiment provides a heterogeneous decellularized bone repair material and a preparation method thereof. The preparation method comprises the steps of:

[0050] (1) Cut the bovine cancellous bone into 5mm granular shapes, wash it repeatedly with a high-pressure water gun until the color of the bone tissue is white to yellowish, and there is no visible bone marrow tissue, and dry it in a drying oven for 12 hours.

[0051] (2) Soak the dried cancellous bone in 35% hydrogen peroxide at 4° C. for 18 h with the mass fraction of the dried cancellous bone in step (1), wash the soaked cancellous bone repeatedly with deionized water, and place in a drying oven Let dry for 8 hours.

[0052] (3) Soak the cancellous bone dried in step (2) with a 0.6M sodium hydroxide solution at 40°C for 20 hours, wash the soaked cancellous bone repeatedly with distilled water, and dry it in a drying oven for 8 hours .

[0053] (4) Place the cancellous bone dried in step (3) together with ...

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Abstract

The present invention provides a biological type bone repairing material and a preparation method thereof, and particularly discloses a heterogeneous decellularized bone repairing material and a preparation method and an application thereof. The preparation method comprises the following steps: (1) cancellous bones of mammals are taken and crushed into granules, the cancellous bone granules are rinsed until no visible bone marrow exists, and the rinsed cancellous bone granules are dried; (2) the obtained bone repairing material in the step (1) is placed in hydrogen peroxide to be soaked, and rinsing and drying are conducted; (3) the obtained bone repairing material in the step (2) is placed in a sodium hydroxide solution to be soaked, and rinsing and drying are conducted; and (4) the obtained bone repairing material in the step (3) is placed in a mixed solution of ethanol and acetone to be soaked, and rinsing and drying are conducted to obtain the heterogeneous decellularized bone repairing material. Compressive strength and elasticity modulus of the prepared heterogeneous decellularized bone repairing material by the technology are consistent with compressive strength and elasticity modulus of human body cancellous bones. The biological type bone repairing material is very suitable for repairing human body bone defects.

Description

technical field [0001] The invention relates to the technical field of biological repair, and relates to a biological bone repair material and a preparation method thereof, in particular to a heterogeneous decellularized bone repair material, a preparation method and an application thereof. Background technique [0002] In recent years, the application of biosynthetic materials in bone defect repair has become a research hotspot. With the in-depth study of molecular biology, the current consensus is that bone defect repair is a long histological process accompanied by extremely complex and precise biological regulation. Simply put, it is the theory of crawling replacement. This theory holds that when the bone substitute biological repair material is filled into the bone defect area to be repaired, it is first soaked in blood, and then under the environment created by the blood clot, the filled bone substitute material Osteoconductivity is to provide scaffolding for the host...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/56A61L27/50
CPCA61L27/3633A61L27/365A61L27/3687A61L27/50A61L27/56A61L2430/02
Inventor 凌友杨景徐斌
Owner SUMMIT GD BIOTECH
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