Artemisia apiacea essential oil and white tea essential oil for promoting skin cell regeneration and composition of artemisia apiacea essential oil and white tea essential oil

A technology of skin cells and tea essential oil, which can be used in skin care preparations, drug combinations, skin diseases, etc., can solve the problems that have not been seen in the research of Artemisia annua essential oil, achieve good application prospects, improve activity, and ensure the effect of repair and regeneration

Active Publication Date: 2021-12-10
梵申兰科技(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, there is currently no research on the effect of Artemisia annua essential oil and green tea essential oil on promoting skin regeneration

Method used

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  • Artemisia apiacea essential oil and white tea essential oil for promoting skin cell regeneration and composition of artemisia apiacea essential oil and white tea essential oil
  • Artemisia apiacea essential oil and white tea essential oil for promoting skin cell regeneration and composition of artemisia apiacea essential oil and white tea essential oil
  • Artemisia apiacea essential oil and white tea essential oil for promoting skin cell regeneration and composition of artemisia apiacea essential oil and white tea essential oil

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Effect test

Embodiment 1

[0029] Embodiment 1 Artemisia annua essential oil of the present invention and preparation thereof (1)

[0030] Collect fresh young Artemisia annua seedlings in May in Henan area, remove the stems and useless parts, wash the leaves, filter out the water, and dry them in an electric constant temperature drying oven. The temperature is controlled below 60 ° C, and the drying time is 3 hours; Cut the dried Artemisia annua into 4-6mm pieces with a cutting machine, add 5 times the amount of distilled water, adjust the pH to 10, add alkaline protease at a dosage of 100U / g, react in a water bath at 50°C for 3 hours, and then adjust the pH to 4.8, add cellulase, the dosage is 20U / g, react in a water bath at 50°C for 3h; after enzymolysis, centrifuge at 3000r / min for 20min, collect the liquid phase, extract with ether, collect the upper layer, and dehydrate with anhydrous sodium sulfate , 50 ℃ constant temperature water bath to evaporate ether.

Embodiment 2

[0031] Embodiment 2 Artemisia annua essential oil of the present invention and its preparation (two)

[0032] Collect fresh young Artemisia annua seedlings in May in Henan area, remove the stems and useless parts, wash the leaves, filter out the water, and dry them in an electric constant temperature drying oven. The temperature is controlled below 60 ° C, and the drying time is 3 hours; Cut the dried Artemisia annua into 4-6mm pieces with a cutting machine, add 5 times the amount of distilled water, adjust the pH to 10, add alkaline protease at a dosage of 80U / g, react in a water bath at 50°C for 3 hours, and then adjust the pH to 4.8, add cellulase, the dosage is 20U / g, react in a water bath at 50°C for 3h; after enzymolysis, centrifuge at 3000r / min for 20min, collect the liquid phase, extract with ether, collect the upper layer, and dehydrate with anhydrous sodium sulfate , 50 ℃ constant temperature water bath to evaporate ether.

Embodiment 3

[0033] Embodiment 3 Artemisia annua essential oil of the present invention and preparation thereof (three)

[0034]Collect fresh young Artemisia annua seedlings in May in Henan area, remove the stems and useless parts, wash the leaves, filter out the water, and dry them in an electric constant temperature drying oven. The temperature is controlled below 60 ° C, and the drying time is 3 hours; Cut the dried Artemisia annua into 4-6mm pieces with a cutting machine, add 5 times the amount of distilled water, adjust the pH to 10, add alkaline protease at a dosage of 120U / g, react in a water bath at 50°C for 3 hours, and then adjust the pH to 4.8, add cellulase, the dosage is 20U / g, react in a water bath at 50°C for 3h; after enzymolysis, centrifuge at 3000r / min for 20min, collect the liquid phase, extract with ether, collect the upper layer, and dehydrate with anhydrous sodium sulfate , 50 ℃ constant temperature water bath to evaporate ether.

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Abstract

The invention relates to artemisia apiacea essential oil and white tea essential oil for promoting skin cell regeneration and a composition of the artemisia apiacea essential oil and the white tea essential oil. A preparation method for the Artemisia apiacea essential oil comprises the following steps: collecting fresh Artemisia apiacea tender shoots, taking leaves, drying the leaves, cutting the leaves into segments, carrying out enzymolysis by using alkaline protease and cellulase in sequence, and carrying out centrifugation and extraction. A preparation method for the white tea essential oil comprises the following steps: collecting fresh white tea tender shoots, withering the fresh white tea tender shoots in a backlight room under the conditions that a temperature is 30-35 DEG C and humidity is 50-60% until a weight loss rate is 40-60%, conducting drying and crushing, carrying out enzymolysis by using pectinase and cellulase in sequence, and carrying out centrifuging and extracting. It is proved in the invention that the artemisia apiacea essential oil, the white tea essential oil and the composition of the artemisia apiacea essential oil and the white tea essential oil have obvious promotion effects on HaCat cell migration, proliferation of human skin fibroblasts and epidermal stem cells and the capability of synthesizing type I collagen from the human skin fibroblasts, and have application potential in the aspect of resisting skin aging.

Description

technical field [0001] The invention relates to plant essential oil extraction, in particular to a kind of artemisia annua essential oil, white tea essential oil and their composition for promoting skin cell regeneration, preparation method, and application thereof. Background technique [0002] With the improvement of living material level, people's demand for skin anti-aging is increasing day by day. Skin aging is the result of various factors. According to the factors that cause skin aging, skin aging can be classified into two types: one is endogenous aging, also known as natural aging, which is mainly affected by genetic factors and is natural gradual aging. The other is exogenous aging caused by external environmental factors such as ultraviolet radiation, smoking, gravity, and chemicals. More than 80% of facial skin aging is due to ultraviolet radiation, so it is also called photoaging. This type of skin aging is characterized by rough skin texture, dull complexion, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B9/02C11B9/00A61K8/92A61Q19/08A61K36/82A61K36/282A61P17/18
CPCC11B9/02C11B9/025C11B9/022C11B9/00A61K8/922A61Q19/08A61K36/82A61K36/282A61P17/18A61K2236/19A61K2800/805A61K2300/00
Inventor 汤凯
Owner 梵申兰科技(上海)有限公司
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