Artemisia apiacea essential oil and white tea essential oil for promoting skin cell regeneration and composition of artemisia apiacea essential oil and white tea essential oil
A technology of skin cells and tea essential oil, which can be used in skin care preparations, drug combinations, skin diseases, etc., can solve the problems that have not been seen in the research of Artemisia annua essential oil, achieve good application prospects, improve activity, and ensure the effect of repair and regeneration
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Embodiment 1
[0029] Embodiment 1 Artemisia annua essential oil of the present invention and preparation thereof (1)
[0030] Collect fresh young Artemisia annua seedlings in May in Henan area, remove the stems and useless parts, wash the leaves, filter out the water, and dry them in an electric constant temperature drying oven. The temperature is controlled below 60 ° C, and the drying time is 3 hours; Cut the dried Artemisia annua into 4-6mm pieces with a cutting machine, add 5 times the amount of distilled water, adjust the pH to 10, add alkaline protease at a dosage of 100U / g, react in a water bath at 50°C for 3 hours, and then adjust the pH to 4.8, add cellulase, the dosage is 20U / g, react in a water bath at 50°C for 3h; after enzymolysis, centrifuge at 3000r / min for 20min, collect the liquid phase, extract with ether, collect the upper layer, and dehydrate with anhydrous sodium sulfate , 50 ℃ constant temperature water bath to evaporate ether.
Embodiment 2
[0031] Embodiment 2 Artemisia annua essential oil of the present invention and its preparation (two)
[0032] Collect fresh young Artemisia annua seedlings in May in Henan area, remove the stems and useless parts, wash the leaves, filter out the water, and dry them in an electric constant temperature drying oven. The temperature is controlled below 60 ° C, and the drying time is 3 hours; Cut the dried Artemisia annua into 4-6mm pieces with a cutting machine, add 5 times the amount of distilled water, adjust the pH to 10, add alkaline protease at a dosage of 80U / g, react in a water bath at 50°C for 3 hours, and then adjust the pH to 4.8, add cellulase, the dosage is 20U / g, react in a water bath at 50°C for 3h; after enzymolysis, centrifuge at 3000r / min for 20min, collect the liquid phase, extract with ether, collect the upper layer, and dehydrate with anhydrous sodium sulfate , 50 ℃ constant temperature water bath to evaporate ether.
Embodiment 3
[0033] Embodiment 3 Artemisia annua essential oil of the present invention and preparation thereof (three)
[0034]Collect fresh young Artemisia annua seedlings in May in Henan area, remove the stems and useless parts, wash the leaves, filter out the water, and dry them in an electric constant temperature drying oven. The temperature is controlled below 60 ° C, and the drying time is 3 hours; Cut the dried Artemisia annua into 4-6mm pieces with a cutting machine, add 5 times the amount of distilled water, adjust the pH to 10, add alkaline protease at a dosage of 120U / g, react in a water bath at 50°C for 3 hours, and then adjust the pH to 4.8, add cellulase, the dosage is 20U / g, react in a water bath at 50°C for 3h; after enzymolysis, centrifuge at 3000r / min for 20min, collect the liquid phase, extract with ether, collect the upper layer, and dehydrate with anhydrous sodium sulfate , 50 ℃ constant temperature water bath to evaporate ether.
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