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Application of a kind of Rhodococcus rhodochrous and its preparation in the remediation of pyrethroid pesticide pollution

A technology of pyrethroids and rhodococcus, which is applied in the restoration of polluted soil, water pollutants, bacteria, etc., can solve the problems of lack of bacterial strains, and achieve the advantages of convenient use, protection of the ecological environment and human health, and low production costs Effect

Active Publication Date: 2022-07-29
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, a few countries and enterprises have realized the development and application of microbial degradation agents, but there is still a lack of more strains with excellent bioremediation potential

Method used

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  • Application of a kind of Rhodococcus rhodochrous and its preparation in the remediation of pyrethroid pesticide pollution
  • Application of a kind of Rhodococcus rhodochrous and its preparation in the remediation of pyrethroid pesticide pollution
  • Application of a kind of Rhodococcus rhodochrous and its preparation in the remediation of pyrethroid pesticide pollution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Isolation and identification of bacterial strains

[0047] 1. Isolation and screening of strains

[0048] Sample: Collected from activated sludge near a chemical plant in Guangdong Province.

[0049] The separation and screening method adopts the enrichment culture method, as follows:

[0050] Take 10 g of activated sludge sample and add it to 50 mL of basal medium. At the same time, add d-cyfenothrin mother solution (acetone is the solvent), so that the final mass concentration of d-cyfenothrin is 50 mg / L, at 30 ° C, 200 r Tolerant bacteria enrichment culture was carried out under a shaker / min. After 7 days of culture, the cells were transferred to a second batch of MSM medium containing 100 mg / L dexfenothrin at 10% of the inoculum. After culturing for 7 days under the same conditions, the cells were then transferred to MSM containing 200 mg / L of dexfenothrin at a 10% inoculum amount, and the culture was continued for 7 days. By analogy, the mass concentr...

Embodiment 2

[0057] The preparation of embodiment 2 strain Y14 degrading bacterial agent

[0058] 1. The production process flow of preparing the degrading bacterial agent using the above-mentioned isolated strain Y14 is:

[0059] Bacteria - shake flask seed liquid - seed tank culture - production tank fermentation - product (the dosage form can be suspension or powder).

[0060] 2. The specific method is as follows:

[0061] A strain of strain Y14 was activated on LB solid plates for 48 hours. The activated strain Y14 was inoculated into a 1000 mL conical flask containing 250 mL of LB medium, and incubated at 30°C with constant temperature shaking to a logarithmic growth phase. Medium, the filling volume is 50-80%. Inoculate 250 mL of the cultured bacterial liquid in a seed tank with a liquid filling volume of 70% according to the inoculum volume of 10%, and the ventilation volume of the sterile air is 0.6-1.0m 3 / min, the stirring speed is 120 ~ 200r / min, and cultivated to the logari...

Embodiment 3

[0063] Example 3 The optimal degradation process conditions of Y14 bacterial agent

[0064] The Design Expert software was used to design the experiment according to the Box-Behnken design principle of the response surface method, as shown in Table 1. Selection of relevant factors affecting the growth and degradation of strain Y14 temperature (X 1 ), pH value (X 2 ) and inoculation amount (X 3 ) as the independent variable, taking the residual amount of dexfenothrin as the response value (Y 1 ) is the dependent variable to construct a quadratic polynomial regression equation. Then, according to the quadratic polynomial regression equation, the three-dimensional response surface map of the degradation of dextrofenothrin by strain Y14 was obtained. Finally, the first-order partial derivative of the quadratic polynomial regression equation was obtained, and the extreme point of the model was obtained by solving the equation, that is, the optimum degradation process conditions...

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Abstract

The invention discloses the application of Rhodococcus rhodochrous and its preparation in the remediation of pyrethroid pesticide pollution. The strain is the gram-positive bacterium Rhodococcus ruber strain Y14, and was deposited in the Guangdong Provincial Microbial Culture Collection Center on July 21, 2021, and the deposit number is GDMCC NO: 61813. The strain has significant biodegradation ability of pyrethroid insecticides, especially the highest activity to dextrofenothrin, and the number of bacterial cells is not less than 1.0×10. 6 CFU·mL ‑1 The suspension of 50mg·L in water after 6 days of treatment ‑1 D-Cyphenothrin is completely removed, and it also has a high-efficiency bioremediation effect on polluted soil. It provides a new idea to solve the problems of excessive pyrethroid pesticide residues and environmental pollution in agricultural production, and also enriches the germplasm resource bank of pesticide-degrading bacteria, which is of great practical importance for the production of non-toxic and pollution-free green agricultural products. Value.

Description

technical field [0001] The invention belongs to the technical field of microbial degradation. More specifically, it relates to the application of Rhodococcus rhodochrous and its preparation in the remediation of pyrethroid pesticide pollution. Background technique [0002] Pyrethroid Insecticides A synthetic analogue of natural pyrethrins, the first generation of which was synthetic allethrin in 1949. However, it was later found that the synthetic pyrethroids were photo-labile, and were prone to photolysis and failure in the natural environment. Therefore, in the 1970s, a cyano group was introduced at the alpha chiral carbon site in the pyrethroid structure, resulting in the second generation of pyrethroid insecticides. So far, pyrethroid insecticides have been used worldwide for half a century. With the phasing out of highly toxic and high residue carbamate and organophosphorus pesticides by the World Health Organization, the global market share of pyrethroid pesticides ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/01C02F101/38C02F101/36C02F101/34
CPCC12N1/20B09C1/10C02F3/34C02F2101/306C02F2101/34C02F2101/36C02F2101/38
Inventor 陈少华黄耀华周喆吴小珍余虹晓张国瑞
Owner SOUTH CHINA AGRI UNIV
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