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A kind of pyrethroid insecticide residue degradation bacterial strain and its application

A technology for pyrethroids and pesticides, which is applied in the field of pyrethroid pesticide residues degrading strains, can solve the problems of not being able to meet the actual needs of biodegradation of chemical pesticide residue pollution, and failing to degrade allethrin, etc. The effect of good biodegradation

Active Publication Date: 2020-12-29
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For example, patent 201710927602.5 discloses a compound bacterial agent and its use in degrading pyrethroid pesticide residues in soil. Using domestication technology, Sphingomonas, Bacillus licheniformis, Rhodopseudomonas palustris, and coccus and Enterobacter cloacae, domesticated to prepare a compound bacterial agent, which can degrade bifenthrin, fenpropathrin and cypermethrin; but it does not degrade allethrin; moreover, the existing resource bank of degrading bacteria is far from Can not meet the actual needs of biodegradation of chemical pesticide residue pollution
In particular, there is currently a lack of degradation preparations for allethrins and other pyrethroid pesticides.

Method used

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  • A kind of pyrethroid insecticide residue degradation bacterial strain and its application
  • A kind of pyrethroid insecticide residue degradation bacterial strain and its application
  • A kind of pyrethroid insecticide residue degradation bacterial strain and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Isolation and Identification of Example 1 Bacterial Strain CW3

[0053] 1. Isolation and screening of strains

[0054] (1) Sample: collected from activated sludge near a chemical plant in Guangxi Province.

[0055] (2) The separation and screening method adopts the enrichment culture method, as follows:

[0056] Take 5g of activated sludge sample and add it to 50mL basal medium. At the same time, add the mother liquor of allethrin (acetone is the solvent) so that the final mass concentration of allethrin is 50mg / L, and degrade it at 30°C and 200r / min on a shaking table Bacterial enrichment culture. After culturing for 7 days, transfer to the second batch of MSM medium containing 100 mg / L allethrin according to the inoculum size of 10%. After culturing under the same conditions for 7 days, transfer to MSM containing 200 mg / L allethrin according to 10% inoculum size, and continue culturing for 7 days. By analogy, the mass concentration of allethrin is continuously dou...

Embodiment 2

[0061] The preparation of embodiment 2 bacterial strain CW3 degrading bacterial agent

[0062] 1. The production process of using the above-mentioned bacterial strain CW3 to prepare the degrading bacterial agent is as follows: slant strain—shake flask seed liquid—seed tank cultivation—production tank fermentation—product (dosage form can be suspension or powder).

[0063] 2. The specific method is as follows:

[0064] (1) Activate the bacterial strain CW3 on the LB solid plate, inoculate it on the inclined surface of the LB test tube for later use.

[0065] (2) Inoculate the test tube seed of strain CW3 in a 1000mL shake flask containing 250mL LB medium, shake at a constant temperature of 30°C to the logarithmic phase, and inoculate the obtained bacterial solution into a seed tank, which contains sterilized seeds Culture medium, the filling volume is 70%. Inoculate the cultivated shake flask bacterial liquid with 10% inoculum volume into the seed tank with 70% liquid volume,...

Embodiment 3

[0067] Embodiment 3CW3 inoculum optimal degradation process condition

[0068] 1. Carry out a single factor degradation test of the CW3 bacterial agent, and measure the degradation rate of the CW3 bacterial agent by sequentially changing the factors that affect growth and degradation, such as temperature, pH value, degradation concentration, inoculum volume, liquid filling volume, oscillation rate, etc. Determine the key factors affecting the degradation rate of CW3 inoculum.

[0069] Utilize Design Expert 12.0 software to carry out experimental design (table 1) according to the principle of response surface method Box-Behnken design, to influence the key factor pH value (X 1 ), temperature (X 2 ) and degradation concentration (X 3 ) is the independent variable, and the allethrin residue is the response value (Y 1 ) to establish a multiple quadratic regression equation.

[0070] According to the multiple quadratic regression equation for drawing analysis, the response surf...

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Abstract

The invention discloses a pyrethroid insecticide residual degradation bacterial strain and application thereof. The strain is Sphingomonas trueperi strain CW3, which was deposited in Guangdong Microbial Culture Collection Center on November 13, 2019, with the preservation number GDMCC NO: 60893. The strain has significant potential to efficiently degrade pyrethroid insecticides such as allethrin, and the strain can be prepared as a degrading bacterial agent, which has the characteristics of low production cost, safety and high efficiency, and convenient use, and can be used to repair allethrins. The natural environment polluted by pyrethroid pesticides; after 7 days of direct application treatment, the residual amount of allethrin in the water can be reduced by more than 93%, and it also has a high-efficiency bioremediation effect on the soil. It provides a new idea for the problem of excessive pesticide residues and environmental pollution, and has important theoretical guidance and practical application value for the production of non-toxic and pollution-free green agricultural products.

Description

technical field [0001] The invention belongs to the technical field of microbial degradation. More specifically, it relates to a pyrethroid insecticide residue degrading strain and application thereof. Background technique [0002] Pyrethroid insecticides are widely used worldwide for crop yield enhancement and household hygiene pest control. However, the widespread use of pyrethroids can cause environmental damage and affect human health. During the application process, pesticides will disperse in the air, water and soil environment, enter groundwater, and enter the food chain through drinking water, thereby affecting the health of non-target organisms. Pyrethroids have a long residual effect in the environment, but these pesticide residues can be removed by physicochemical degradation methods and microbial degradation methods. Natural pyrethrins are derived from plants (pyrethrum) and pyrethroids are developed from natural pyrethrins, both pesticides can be used for ins...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/00C12R1/01A62D101/04A62D101/47
CPCA62D3/02A62D2101/04A62D2101/28C12N1/20C12N1/205C12R2001/01
Inventor 陈少华潘卡·巴特黄耀华张文平林子秋齐媛
Owner SOUTH CHINA AGRI UNIV
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