Rhodococcus ruber and application of preparation thereof in pyrethroid pesticide pollution remediation
A technology of pyrethroids and rhodococcus, which is applied in the restoration of polluted soil, water pollutants, bacteria, etc., can solve the problems of lack of bacterial strains, and achieve the advantages of convenient use, protection of the ecological environment and human health, and low production costs Effect
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Embodiment 1
[0046] The isolation and identification of embodiment 1 bacterial strain
[0047] 1. Isolation and screening of bacterial strains
[0048] Sample: Collected from activated sludge near a chemical plant in Guangdong Province.
[0049] The separation and screening method adopts the enrichment culture method, as follows:
[0050] Take 10g of activated sludge sample and add it to 50mL basal medium, and add d-cyphenothrin mother liquor (acetone is solvent) at the same time, so that the final mass concentration of d-cyphenothrin is 50mg / L, at 30°C, 200r / min shaker for enrichment culture of resistant bacteria. After culturing for 7 days, transfer to the second batch of MSM medium containing 100 mg / L d-cyphenothrin according to 10% inoculum size. After culturing under the same conditions for 7 days, the inoculum was transferred to MSM containing 200 mg / L of dex-cyphenothrin at a concentration of 200 mg / L according to the inoculum size of 10%, and the culturing was continued for 7 d...
Embodiment 2
[0057] The preparation of embodiment 2 bacterial strain Y14 degrading bacteria agent
[0058] 1. The production process of using the above-mentioned isolated bacterial strain Y14 to prepare the degrading bacterial agent is as follows:
[0059] Strains—shake flask seed solution—seed tank culture—production tank fermentation—product (dosage form can be suspension or powder).
[0060] 2. The specific method is as follows:
[0061] The strain Y14 was activated on LB solid plates for 48 hours. Inoculate the activated strain Y14 into a 1000mL Erlenmeyer flask containing 250mL of LB medium, culture at a constant temperature and shake at 30°C to the logarithmic phase of growth, and inoculate the obtained bacterial solution into a seed tank, which contains sterilized seeds Culture medium, the filling volume is 50-80%. Inoculate 250mL of the cultured bacterial solution into a seed tank with a liquid volume of 70% at an inoculum volume of 10%, and the ventilation volume of the sterile...
Embodiment 3
[0063] Embodiment 3 Y14 inoculum optimal degradation process condition
[0064] Design Expert software was used to carry out experimental design according to the Box-Behnken design principle of response surface method, see Table 1 for details. Select the relevant factor temperature (X 1 ), pH value (X 2 ) and inoculation amount (X 3 ) as an independent variable, with d-cyphenothrin residue as a response value (Y 1 ) is the dependent variable, constructing a quadratic polynomial regression equation. Then, according to the quadratic polynomial regression equation, the three-dimensional response surface plot of strain Y14 degrading d-cyphenothrin was obtained. Finally, the first-order partial derivative of the quadratic polynomial regression equation was obtained, and the extreme point of the model was obtained by solving the equation, that is, the optimal degradation process condition for the degradation of d-cyphenothrin by the Y14 bacterial agent.
[0065] Through the ana...
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