Rhodococcus ruber and application of preparation thereof in pyrethroid pesticide pollution remediation

A technology of pyrethroids and rhodococcus, which is applied in the restoration of polluted soil, water pollutants, bacteria, etc., can solve the problems of lack of bacterial strains, and achieve the advantages of convenient use, protection of the ecological environment and human health, and low production costs Effect

Active Publication Date: 2022-03-01
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, a few countries and enterprises have realized the development and application of microbial degradation agents, but there is still a lack of more strains with excellent bioremediation potential

Method used

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  • Rhodococcus ruber and application of preparation thereof in pyrethroid pesticide pollution remediation
  • Rhodococcus ruber and application of preparation thereof in pyrethroid pesticide pollution remediation
  • Rhodococcus ruber and application of preparation thereof in pyrethroid pesticide pollution remediation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The isolation and identification of embodiment 1 bacterial strain

[0047] 1. Isolation and screening of bacterial strains

[0048] Sample: Collected from activated sludge near a chemical plant in Guangdong Province.

[0049] The separation and screening method adopts the enrichment culture method, as follows:

[0050] Take 10g of activated sludge sample and add it to 50mL basal medium, and add d-cyphenothrin mother liquor (acetone is solvent) at the same time, so that the final mass concentration of d-cyphenothrin is 50mg / L, at 30°C, 200r / min shaker for enrichment culture of resistant bacteria. After culturing for 7 days, transfer to the second batch of MSM medium containing 100 mg / L d-cyphenothrin according to 10% inoculum size. After culturing under the same conditions for 7 days, the inoculum was transferred to MSM containing 200 mg / L of dex-cyphenothrin at a concentration of 200 mg / L according to the inoculum size of 10%, and the culturing was continued for 7 d...

Embodiment 2

[0057] The preparation of embodiment 2 bacterial strain Y14 degrading bacteria agent

[0058] 1. The production process of using the above-mentioned isolated bacterial strain Y14 to prepare the degrading bacterial agent is as follows:

[0059] Strains—shake flask seed solution—seed tank culture—production tank fermentation—product (dosage form can be suspension or powder).

[0060] 2. The specific method is as follows:

[0061] The strain Y14 was activated on LB solid plates for 48 hours. Inoculate the activated strain Y14 into a 1000mL Erlenmeyer flask containing 250mL of LB medium, culture at a constant temperature and shake at 30°C to the logarithmic phase of growth, and inoculate the obtained bacterial solution into a seed tank, which contains sterilized seeds Culture medium, the filling volume is 50-80%. Inoculate 250mL of the cultured bacterial solution into a seed tank with a liquid volume of 70% at an inoculum volume of 10%, and the ventilation volume of the sterile...

Embodiment 3

[0063] Embodiment 3 Y14 inoculum optimal degradation process condition

[0064] Design Expert software was used to carry out experimental design according to the Box-Behnken design principle of response surface method, see Table 1 for details. Select the relevant factor temperature (X 1 ), pH value (X 2 ) and inoculation amount (X 3 ) as an independent variable, with d-cyphenothrin residue as a response value (Y 1 ) is the dependent variable, constructing a quadratic polynomial regression equation. Then, according to the quadratic polynomial regression equation, the three-dimensional response surface plot of strain Y14 degrading d-cyphenothrin was obtained. Finally, the first-order partial derivative of the quadratic polynomial regression equation was obtained, and the extreme point of the model was obtained by solving the equation, that is, the optimal degradation process condition for the degradation of d-cyphenothrin by the Y14 bacterial agent.

[0065] Through the ana...

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Abstract

The invention discloses application of rhodococcus ruber and a preparation thereof in pyrethroid pesticide pollution remediation. The bacterial strain is a gram positive bacterium Rhodococcus ruber bacterial strain Y14, and is preserved in Guangdong Microbial Culture Collection Center on July 21, 2021, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 61813. The strain has remarkable pyrethroid insecticide biodegradation capacity, especially has the highest activity on D-cyhalothrin, is prepared into a suspension with the thallus number of not less than 1.0 * 10 < 6 > CFU.mL <-1 >, can completely remove 50 mg.L <-1 > D-cyhalothrin in a water body after being treated for 6 days, and also has an efficient bioremediation effect on polluted soil. The invention provides a new thought for solving the problems of excessive pyrethroid pesticide residue and environmental pollution in agricultural production, enriches the germplasm resource library of pesticide degrading bacteria, and has important practical application value for producing non-toxic and pollution-free green agricultural products.

Description

technical field [0001] The invention belongs to the technical field of microbial degradation. More specifically, it relates to the application of a rhodococcus erythrococcus and its preparation in remediation of pyrethroid pesticide pollution. Background technique [0002] Pyrethroid insecticides are synthetic analogs of natural pyrethrins, the first generation of which was allethrin synthesized in 1949. However, it was later discovered that synthetic pyrethroids are photo-instability and are prone to photolysis and failure in the natural environment. Therefore, in the 1970s, a cyano group was introduced at the α-chiral carbon site in the pyrethroid structure, thus resulting in the second generation of pyrethroid insecticides. So far, pyrethroid insecticides have been used worldwide for half a century. With the phase-out of highly toxic and high residue carbamate and organophosphorus pesticides by the World Health Organization, the global market share of pyrethroid pestic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/01C02F101/38C02F101/36C02F101/34
CPCC12N1/20B09C1/10C02F3/34C02F2101/306C02F2101/34C02F2101/36C02F2101/38
Inventor 陈少华黄耀华周喆吴小珍余虹晓张国瑞
Owner SOUTH CHINA AGRI UNIV
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