Method for separating high-cadmium-resistance sulfate reducing bacteria

A sulfate, cadmium-resistant technology, applied in the methods of using microorganisms, separating microorganisms, and restoring polluted soils, can solve the problems of other dominant bacteria occupying places, difficult to achieve, and sulfate-reducing bacteria cannot grow. achieve a highly tolerated effect

Inactive Publication Date: 2019-11-22
北京本农环保科技集团有限公司
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Problems solved by technology

[0005] The objective disadvantages of the prior art: when cultivating and isolating and purifying sulfate-reducing bacteria, strict anaerobic conditions are easy to achieve in the laboratory, but it is difficult to realize in actual production. If the enrichment is not in place, it is easy for other Dominant bacteria occupy space, and sulfate-reducing bacteria cannot grow

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  • Method for separating high-cadmium-resistance sulfate reducing bacteria

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Embodiment Construction

[0027] The present application will be described in further detail below in conjunction with the accompanying drawings, but it is not intended to limit the present application.

[0028] Such as figure 1 Shown, at first, the sample of the present invention comes from the cadmium-contaminated site. The first step in the isolation process is to activate the sample, ie enrichment. The enrichment medium used in this step of the present invention is a special medium for sulfate-reducing bacteria. The bacteria enrichment is divided into three times. The first enrichment uses a special culture medium for sulfate-reducing bacteria that is not sterilized, and the inoculation amount is not less than 10%. Special culture medium for sulfate-reducing bacteria.

[0029] After the enrichment, it was found that black precipitates were produced in the culture bottle, bubbles were produced at the mouth of the bottle, and there was an odor at the mouth of the bottle, indicating that a large nu...

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Abstract

The invention discloses a method for separating high-cadmium-resistance sulfate reducing bacteria. The method comprises the following steps that 1) a sulfate reducing bacteria sample from a cadmium polluted site is activated; 2) the sulfate reducing bacteria sample is separated and purified; 3) a cadmium-resistance strain is screened; 4) the high-cadmium-resistance bacteria is subjected to oxygen-resistant domestication; 5) testing is conducted on a laboratory scale.

Description

technical field [0001] The invention relates to the technical field of bacteria strain isolation, in particular to a method for isolating highly cadmium-tolerant sulfate-reducing bacteria. Background technique [0002] Sulfate-reducing bacteria (Soulfate-Reducing Bacteria, referred to as SRB) is an anaerobic microorganism. Widely present in anoxic environments such as soil, seawater, river water, underground pipelines, and oil and gas wells. Studies have shown that in the absence of oxygen or very little oxygen, it can use the organic matter on the metal surface as a carbon source, and use the hydrogen produced in the bacterial biofilm to reduce sulfate to hydrogen sulfide, and obtain energy for survival from redox reactions . [0003] Heavy metal cadmium pollution in farmland in southern my country is serious, and cadmium content in agricultural products seriously exceeds the standard. Since cadmium is a toxic element, its compounds are more toxic. In nature, cadmium co...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/36C12N1/02B09C1/10
CPCB09C1/10C12N1/02C12N1/36
Inventor 韩建均王玉文赵川
Owner 北京本农环保科技集团有限公司
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