Preparation method of microbial insecticide

A technology of microbial pesticides and fermentation methods, which is applied in the field of preparation of microbial pesticides, can solve the problems of single application range, strong antagonism, immature industrial production, etc., and achieve the effect of increasing growth rate and promoting plant growth

Active Publication Date: 2017-10-20
上海乾界生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its growth and reproduction are largely restricted by external conditions, and it is restricted by the environment in practical applications.
3. Viral insecticides: The infection routes of insect viruses mainly include ingestion infection and skin infection. At present, this type of preparation is in the research and development stage, and industrial production is not yet mature.
[0004] In summary, the existing microbial insecticides have a relatively single application range in the actual application process, strong antagonism between strains, and a narrow insecticidal spectrum; existing products in terms of industrial application, environmental adaptability, and stress resistance There are also many deficiencies, which limit the wide application of microbial agents on crop insecticides.

Method used

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  • Preparation method of microbial insecticide
  • Preparation method of microbial insecticide
  • Preparation method of microbial insecticide

Examples

Experimental program
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Effect test

preparation example Construction

[0046] The preparation method of the Acorus calamus extract in the examples is: pulverize Acorus calamus and pass through a 16-mesh sieve to obtain Acorus calamus powder; mix the Acorus calamus powder and water with a solid-liquid mass ratio of 1:20; the ultrasonic power is 150W , Ultrasonic extraction at 60°C for 2 hours at an ultrasonic frequency of 40kHz, centrifugation at 5000 rpm for 20 minutes, take the supernatant and concentrate it under reduced pressure at a vacuum of 0.06MPa and a temperature of 70°C, and concentrate to a density of 1.05 g / mL (25°C) to obtain Acorus calamus extract. Acorus calamus, Latin name: Acorus tatarinowii Schott, is the rhizome of Acorustatarinowii (Acorustatarinowii). Specifically, Shichangpu provided by Anguo Yuejie Chinese Medicinal Materials Co., Ltd. is used.

[0047] The preparation method of the mulberry leaf extract described in the examples is: pulverize the mulberry leaf, pass a 16-mesh sieve to obtain mulberry leaf powder; mix the mul...

Embodiment 1

[0053] Step 1: Incline culture of Burkholderia spp. bacteria

[0054] Burkholderia spp. bacteria were inoculated on a slant culture medium for slant culture, the inoculation mass percentage was 5%, the slant culture temperature was 28°C, the time was 24h, and the quality of each component in the slant culture medium The percentages are: sodium chloride 0.5%, peptone 1%, agar powder 1.8%, the rest is water, adjust the pH to 7.0;

[0055] Step 2: Shake flask culture of Burkholderia spp. bacteria

[0056] Inoculate the culture solution obtained in step 1 into the shake flask culture medium for shake flask culture, the inoculation mass percentage is 10%, the shake flask culture temperature is 28° C., the time is 24 hours, and the rotation speed is 220 rpm. The mass percentage of each component is: 0.2% dipotassium hydrogen phosphate, 0.5% beef extract, 1% peptone, and the rest is water. Adjust the pH to 7.0;

[0057] Step 3: Seed cultivation of Burkholderia spp. bacteria

[0058] The cult...

Embodiment 2

[0064] It is basically the same as embodiment 1, the difference is only:

[0065] The mass percentage of each component in the fermentation medium in the step 4 is: beef extract 0.5%, peptone 1%, calamus extract 0.8%, antifoaming agent 0.005%, and the rest is water. Adjust the pH to 7.0.

[0066] In this example, after 28 hours of fermentation in step 4, the number of bacteria in the fermentation broth reached 5×10 8 cfu / ml. It can be seen that adding Acorus calamus extract to the fermentation medium will speed up the fermentation of Burkholderia spp. bacteria, and the yield of Burkholderia spp. bacteria is higher during the same fermentation time.

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Abstract

The invention belongs to the technical field of agricultural microbial agent, and especially relates to a preparation method of a microbial insecticide. The preparation method comprises following steps: 1, burkholderia bacteria slant cultivation; 2, burkholderia shake-flask culture; 3, burkholderia seed culture; and 4, burkholderia fermentation culture. The microbial insecticide is used in plant growing and breeding process, can be used for degrading harmful substances accumulated in fields after successive plantation, controlling plant insects and diseases, and promoting plant growth, and possesses biological control and biological repairing functions. The burkholderia bacteria are capable of producing a plurality of metabolites with antibacterial activity such as siderophore, azophenylene, pyrrolnitrin, phenylpyrrole, monoterpenoid alkaloids, Cepaciamide A, Cepacidine A, Cepacin A, and can be applied in fields such as biological control and degrading of toxins.

Description

Technical field [0001] The invention relates to the technical field of agricultural microbial inoculants, in particular to a method for preparing microbial insecticides. Background technique [0002] Plant diseases and insect pests are the enemy of agricultural production. According to the statistics of the Food and Agriculture Organization of the United Nations (FAO), the average annual loss of reduced production due to plant diseases is 15%-20% of the total output. For a long time, the preparations used to control or extinguish farmland pests are generally chemical pesticides, such as HCV and dichlorvos. Frequent use of chemical pesticides over a long period of time has led to the emergence of pest resistance, which gradually reduces the effectiveness of chemical pesticides. At present, the main solution is to increase the amount of pesticides used, which directly leads to the increase in agricultural costs; the resistance of pests is strengthened, and the difficulty of preven...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04A01N63/00A01N43/16A01P7/00C12R1/01
CPCA01N43/16A01N63/00C12N1/20C12P1/04C12N1/205C12R2001/01A01N2300/00
Inventor 林江一
Owner 上海乾界生物科技有限公司
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