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Oleophylic fertilizer for bioremediation of oil polluted environment and method for preparing oleophylic fertilizer

A technology for bioremediation and oil pollution, applied in the field of oil pollution environmental control, can solve problems such as inappropriate bioremediation, and achieve the effects of improving bioavailability, promoting bioremediation, and eliminating oil films

Inactive Publication Date: 2012-10-10
NAVY MEDICINE RES INST OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, lipophilic fertilizers used for bioremediation of oil-polluted environments are mainly solid, such as the lipophilic fertilizers for bioremediation of oil-polluted environments invented by Zhu Shengfeng et al. repair

Method used

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  • Oleophylic fertilizer for bioremediation of oil polluted environment and method for preparing oleophylic fertilizer
  • Oleophylic fertilizer for bioremediation of oil polluted environment and method for preparing oleophylic fertilizer
  • Oleophylic fertilizer for bioremediation of oil polluted environment and method for preparing oleophylic fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Preparation of lipophilic fertilizer with urea as nitrogen source

[0034] Prepare a mixture of soybean lecithin and co-emulsifier according to the mass ratio (Km value) of 1:2, heat at 75°C for 10 min, vortex mix for 2 min, and dissolve in ultrasonic for 10 min, weigh oleic acid and the co-emulsifier according to the mass ratio of 3:7 2 g of the mixture was vortexed for 15 min to mix well. Add 600 μl of saturated urea solution, vortex and mix for 10 minutes, and then let it stand for about 30 minutes to balance and form a water-in-oil microemulsion. Sealed and stored to get the product.

Embodiment 2

[0035] Example 2 Adding lipophilic fertilizers to oil-polluted seawater and observing the state of the oil with a microscope

[0036] Get 50ml seawater sample in the Erlenmeyer flask of 150ml, add 0.5ml diesel oil respectively, one bottle adds the lipophilic fertilizer 100 μ l prepared in embodiment 1, another bottle does not add, after shaking at 100rpm rotating speed simultaneously 12h, get sample in Observed under a microscope, under the magnification of 100 times, the state of diesel oil is as follows: figure 1 As shown, the oleophilic fertilizer makes the oil film form small oil droplets and aggregate, and at the same time attach to the oil.

Embodiment 3

[0037] Example 3 The promotion of lipophilic fertilizers to the degradation of diesel by strains

[0038] Four strains of diesel-oil-degrading bacteria isolated from oil-polluted seawater were cultured overnight in LB medium, then the bacteria were collected and inoculated into 150ml Erlenmeyer flasks containing 50ml of seawater samples, and 0.5ml of diesel oil was added to each bottle. 100 μl of the lipophilic fertilizer prepared in Example 1 was not added to the other bottle. After culturing in a shaker at 30° C. and 180 rpm for 4 days, the degradation rate of diesel oil was measured. The degradation rate of diesel oil was only 8% without adding lipophilic fertilizer, but it reached 64% under the condition of adding lipophilic fertilizer.

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Abstract

The invention relates to the technical field of management of oil polluted environments, in particular to an oleophylic fertilizer for bioremediation and a method for preparing the oleophylic fertilizer. The oleophylic fertilizer is composed of lecithin, oleic acid, nitrogen-phosphorus solution and alcohols. The preparation method includes: mixing the lecithin with the alcohols proportionally, heating, ultrasonically dissolving, adding the nitrogen-phosphorus solution and oleic acid of a certain quantity, and performing vortex oscillating to form water-in-oil microemulsion, so that the product is obtained and sealed for storage. After the oleophylic fertilizer contacts with oil pollutants, the oil pollutants can form fine oil drops to be aggregated, the oleophylic fertilizer is attached onto the oil drops to provide nitrogen and phosphorus source needed by oil degrading microorganisms, and bioremediation effect can be remarkably improved.

Description

1 Technical field [0001] The invention relates to the technical field of oil-polluted environment treatment, in particular to an lipophilic fertilizer for bioremediation and a preparation method thereof. After the fertilizer is in contact with petroleum pollutants, it can make the oil film form fine oil droplets and aggregate, while the lipophilic fertilizer adheres to the periphery of the oil droplets and provides the nitrogen and phosphorus sources required for the growth of petroleum-degrading bacteria, which can significantly improve the bioremediation effect. 2 background technology [0002] With the rapid development of the petroleum industry, the damage of petroleum to the environment has attracted more and more attention. Especially for the marine environment, various oil spill accidents have caused serious harm to the marine ecosystem and restricted the sustainable development of human society and the environment. The bioremediation technology of oil pollution is c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05G3/04C05G3/80
Inventor 罗群沈先荣侯登勇何颖张建国刘琼王庆蓉
Owner NAVY MEDICINE RES INST OF PLA
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