Self-assembled nano-capture antibacterial peptide KIL4 as well as preparation method and application thereof
By designing and assembling self-contained nanoparticles to capture the antimicrobial peptide KIL4, the issues of biotoxicity and environmental sensitivity of antimicrobial peptides in clinical applications have been resolved. This has enabled the effective inhibition and capture of Gram-positive and Gram-negative bacteria, providing technical support for novel antimicrobial drugs.
CN121471324APending Publication Date: 2026-02-06NORTHEAST AGRICULTURAL UNIVERSITY
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Patent Information
- Application Number
- CN202511526587.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-10-24
- Publication Date
- 2026-02-06
AI Technical Summary
Technical Problem
Existing antimicrobial peptides face issues of biotoxicity and environmental sensitivity in clinical applications, making it difficult to fully realize their antimicrobial efficacy.
Method used
A self-assembled nanoparticle-capturing antimicrobial peptide, KIL4, was designed. By forming a nanostructure through specific amino acid sequences and self-assembly conditions, its stability and targeted binding ability were enhanced. It was prepared and purified by solid-phase chemical synthesis, and its nanomorphology and biocompatibility were tested.
Benefits of technology
It achieves significant inhibition of both Gram-positive and Gram-negative bacteria, exhibits good biocompatibility and bacterial capture ability, and provides technical support for novel antibacterial drugs.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention discloses a self-assembled nano-capture antibacterial peptide KIL4 and a preparation method and application thereof, and belongs to the technical field of biology, and the amino acid sequence is shown as SEQ ID No.1. The preparation method comprises the following steps: adopting an alpha-spiral heptapeptide repetitive sequence (abcdefg) 4 template, and selecting lysine to provide positive charges at positions b and c; isoleucine and leucine are respectively filled to a position a and a position d to provide intermolecular hydrophobic force, and lysine and glutamic acid are selected to be respectively filled to a position e and a position g to provide intermolecular electrostatic force; the position f of the center of the hydrophilic surface of the polypeptide is filled with tryptophan to optimize the hydrophobicity of the polypeptide, on the basis, the sequence is repeated for four times, and the invention further discloses application of the polypeptide in preparation of drugs for treating gram-positive bacteria or / and gram-negative bacteria infectious diseases. The antibacterial peptide disclosed by the invention has dual effects of resisting bacteria and capturing bacteria, has excellent biocompatibility, and provides an effective technical support for developing novel antibacterial drugs.
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