Microbial sludge deodorizer and preparation method thereof

A technology of microorganisms and deodorants, which is applied in the field of preparation, microbial sludge deodorants and its preparation, can solve the problems of product quality and use effect, the unstable proportion of effective bacteria group, etc., and achieve good thermal stability and easy Preservation and pollution reduction effects

Inactive Publication Date: 2017-03-22
YANCHENG FUHUA ENVIRONMENT PROTECTION IND DEV CO LTED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some even use a single Bacillus subtilis agent as a biological deodorant, and some have complex components, but the proportion of effective bacteria is unstable, resulting in constant problems in product quality and use effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The strains of Streptococcus thermophilus, Bacillus subtilis, Aspergillus oryzae, photosynthetic bacteria, Saccharomyces cerevisiae, Lactobacillus casei and Pseudomonas putida were cultured at high density:

[0026] (1) Streptococcus thermophilus: Inoculate the Streptococcus thermophilus species on the purified cultured slant medium into a shake flask filled with an activation medium with a volume ratio of 15%. The activation medium formula is yeast extract 6.5 g / l, peptone 6.5g / l, glucose 8g / l, dipotassium hydrogen phosphate 1g / l, tomato juice 50ml, pH value 6.5, temperature 50°C, shaking flask speed 160rpm, activation culture for 42h; The Streptococcus thermophilus species is inoculated into a fermenter equipped with a volume ratio of 50% fermentation medium, and the inoculum size is 1% by volume. The formula of the fermentation medium is corncob 5g / l, glucose 8g / l, yeast Paste 8g / l, Potassium dihydrogen phosphate 18g / l, Magnesium sulfate 0.1g / l, Calcium chloride 0.2g...

Embodiment 2

[0035] The strains of Streptococcus thermophilus, Bacillus subtilis, Aspergillus oryzae, photosynthetic bacteria, Saccharomyces cerevisiae, Lactobacillus casei and Pseudomonas putida were cultured at high density:

[0036] (1) Streptococcus thermophilus: Inoculate the Streptococcus thermophilus species on the purified cultured slant medium into a shaker flask filled with an activation medium with a volume ratio of 30%. The activation medium formula is yeast extract 8.0 g / l, peptone 8.0g / l, glucose 12g / l, dipotassium hydrogen phosphate 3g / l, tomato juice 200ml, pH value 7.2, temperature 50°C, shaking flask speed 160rpm, activation culture for 30h; The Streptococcus thermophilus species is inoculated into a fermenter equipped with a volume ratio of 70% fermentation medium, and the inoculum size is 3% by volume. The formula of the fermentation medium is corncob 6g / l, glucose 12g / l, yeast Paste 12g / l, Potassium dihydrogen phosphate 22g / l, Magnesium sulfate 0.5g / l, Calcium chloride...

Embodiment 3

[0045] The strains of Streptococcus thermophilus, Bacillus subtilis, Aspergillus oryzae, photosynthetic bacteria, Saccharomyces cerevisiae, Lactobacillus casei and Pseudomonas putida were cultured at high density:

[0046] (1) Streptococcus thermophilus: Inoculate the Streptococcus thermophilus species on the purified cultured slant medium into a shaker flask filled with an activation medium with a volume ratio of 22%. The activation medium formula is yeast extract 7.8 g / l, peptone 7.8g / l, glucose 10g / l, dipotassium hydrogen phosphate 2g / l, tomato juice 150ml, pH value 7.0, temperature 48°C, shaking flask speed 140rpm, activation culture for 36h; Streptococcus thermophilus species is inoculated into a fermenter that is equipped with a volume ratio of 60% fermentation medium, and the inoculum size is 2% by volume. The formula of the fermentation medium is corncob 5.5g / l, glucose 10g / l, Yeast extract 10g / l, potassium dihydrogen phosphate 20g / l, magnesium sulfate 0.3g / l, calcium ...

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Abstract

The invention discloses a microbial sludge deodorizer and a preparation method thereof. The preparation method comprises the following steps: conducting high-density culture on strains of streptococcus thermophilus, bacillus subtilis, aspergillus oryzae, photosynthetic bacteria, saccharomyces cerevisiae, lactobacillus casei and pseudomonas putida; dehydrating and drying the various single strains which are subjected to high-density culture, so that hypopus microbial dry powder is prepared; and mixing the streptococcus thermophilus, the bacillus subtilis, the aspergillus oryzae, the photosynthetic bacteria, the saccharomyces cerevisiae, the lactobacillus casei and the pseudomonas putida according to weight ratio of 10-20 parts of the streptococcus thermophilus, 10-15 parts of the bacillus subtilis, 20-30 parts of the aspergillus oryzae, 10-15 parts of the photosynthetic bacteria, 10-20 parts of the saccharomyces cerevisiae, 10-15 parts of the lactobacillus casei and 10-20 parts of the pseudomonas putida, so that the microbial sludge deodorizer is obtained. The microbial sludge deodorizer provided by the invention is applicable to garbage deodorization; and in comparison with the prior art, the microbial sludge deodorizer is low in production investment, low in production cost and convenient to use, and moreover, the microbial sludge deodorizer is good in deodorizing effect.

Description

technical field [0001] The invention relates to a preparation method, in particular to a microbial sludge deodorant and a preparation method thereof, belonging to the technical field of environmental protection. Background technique [0002] In recent years, with the improvement of people's awareness of environmental protection, sewage treatment has developed rapidly in our country, but it has brought about another serious environmental protection problem, which is how to properly dispose of the sludge produced after sewage treatment, which has become a It is an urgent problem to be solved in the whole sewage treatment industry. Treated sludge will be a good resource, but it will cause secondary pollution to the environment if it is spread randomly. At present, the best way to deal with sludge is comprehensive utilization, that is, to add inorganic fertilizer, quicklime or fly ash to the sludge, and use it for agricultural production after simple processing. This method is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C12N1/14C02F11/02C12R1/46C12R1/125C12R1/69C12R1/01C12R1/865C12R1/245C12R1/40
CPCC12N1/20C02F11/02C02F2303/02C12N1/14C12N1/18
Inventor 安玉亭顾韵婕
Owner YANCHENG FUHUA ENVIRONMENT PROTECTION IND DEV CO LTED
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