Rooting culture method for poplar tissue culture seedlings
A technology of rooting culture and tissue culture seedlings, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of affecting the success rate, easy to breed miscellaneous bacteria, poor air permeability of the substrate, etc., and achieve convenient preparation , Subculture of rooted seedlings or high success rate of transplanting, and the effect of excellent air permeability of the substrate
Inactive Publication Date: 2012-09-19
HENAN UNIV OF SCI & TECH
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Problems solved by technology
Since sugar is used as the medium, it is easy to breed miscellaneous bacteria, and the air permeability of the substrate is not very good, which affects the success rate of rooting fungus subculture and transplanting
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[0022] Method of the present invention is as follows:
[0023] Materials and Methods
[0024] 1.1 Materials and culture conditions
[0025] 1.2 Method Use commercially available vermiculite with a particle size of 1-3mm as the substrate, rinse it twice with tap water and dry it for later use. Spread the vermiculite in a glass or plastic light-transmitting culture bottle with a thickness of 2-3cm, and add 1-2 times the mass of vermiculite. nutrient solution, the nutrient solution is MS or 1 / 2MS, and the nutrient solution can add plant growth regulator substances naphthalene acetic acid (NAA), indole butyric acid (IBA), indole acetic acid (IAA) 0.01-0.05mg.L -1 , pH value is 6.5. Select 3-5cm high and consistent pumping seedlings for rooting, inoculate 50 plants for each culture medium treatment, culture in a single bottle, and count the rooting rate of each treatment after 30 days. When the aseptic seedlings grow to a height of about 7cm, take out the seedlings with tweezers...
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The invention relates to a rooting culture method for poplar tissue culture seedlings. The method comprises the following steps that: vermiculites are used as substrates and are dried for use after being rinsed for twice, the vermiculites are spread in a light transmission culture bottle to a thickness being 2 to 3cm, nutrition liquid accounting for 1 to 2 times of the weight of the vermiculites is added, uniform-state putting forth seedlings with the height being 3 to 5cm are selected, 50 strains are inoculated and treated in each kind of culture medium, the single-strain and single-bottle culture is adopted, and the respective treated rooting rate is counted after 30 days; and when aseptic seedlings grow to the height about 7cm, young seedlings with parts of vermiculites pasted with the root parts are taken out in a sunlight greenhouse and are transplanted into nutrition soil with the moisture content being 60 to 75 percent, the nutrition soil is tightly pressed, water is sprayed, plastic films are covered for moisture preservation for 7 days, then, the films are gradually opened, and the rooting is observed after 20 days. According to the rooting method provided by the invention, the vermiculites are used for providing the support effect, only 1 / 2MS or MS is added, the preparation is convenient, and the cost is reduced. Because no sugar substance is contained, the sundry fungus propagation cannot easily occur, the ventilation performance of the substrate is good, the rooting is healthy, and the rooting seedling subculture culture or transplanting success rate is high.
Description
technical field [0001] The invention relates to a poplar rooting method, in particular to a poplar tissue culture seedling rooting cultivation method, which belongs to the technical field of plant cultivation. Background technique [0002] Poplar is an important tree species widely distributed in the world and has important economic, ecological and social benefits. Poplar has the characteristics of rapid growth, short rotation period, strong adaptability, and easy reproduction. It is widely used as timber forest, shelter forest and surrounding greening tree species in various places. The significance of poplar tissue culture is mainly manifested in the following aspects: [0003] First, difficult-to-root tree species can be mass-proliferated through tissue culture. For example, poplar tree species, due to the low reproductive rate of root tillers and the difficulty of cutting rooting, cuttings cannot be used for mass propagation, but a large number of tissue culture seedli...
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IPC IPC(8): A01H4/00
Inventor 周洲李永丽崔林开胡艳红范晶超卢伟
Owner HENAN UNIV OF SCI & TECH
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