Microbial soil repairing agent and preparation method thereof

A soil remediation agent and microbial technology, applied in the field of microbial soil remediation agent and its preparation, can solve the problems of secondary pollution, unstable composition, and no effect of anti-replantation, and achieve the effect of high concentration and stable quality

Inactive Publication Date: 2016-02-10
康源绿洲生物科技(北京)有限公司
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AI Technical Summary

Problems solved by technology

The products used to repair soil on the market are mainly chemical ingredients, which are likely to cause secondary pollution after use
At present, EM bacteria are popularized in China, and the effect is not i

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] 6 strains: Rhodopseudomonas palustris, Bacillus subtilis, Saccharomyces cerevisiae, Lactobacillus plantarum, Streptomyces jingyang and Enterococcus faecalis.

[0026] The following are the secondary culture processes of the five strains of bacteria:

[0027] Pseudomonas palustris palustris: KH is the medium for primary culture 2 PO 4 1.0g, CaCl0.1g, NaHCO 3 3.0g, sodium acetate 1.0g, MgCl 2 0.5g, NH 4 Cl1.0g, NaCl1.0g, trace element solution 1.0ml, vitamin solution 1.0ml, sodium succinate 1.0g, yeast extract 0.5g, peptone 0.5g, distilled water 1.0L, inoculum size 1.5-5%, culture pH 6.8, the culture temperature is 30°C, the light intensity is 3500-4500 lux, and the culture time is 5-8 days. The pH value of the culture is 6.8, the culture temperature is 30°C, the light intensity is 3000-4000 lux, and the culture time is 6-8 days.

[0028] The primary culture medium of Bacillus subtilis is 10.0g of peptone, 3.0g of beef extract, 5.0g of NaCl, 1.0L of distilled water,...

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Abstract

The invention relates to a microbial soil repairing agent and a preparation method thereof, and belongs to the technical field of propagation culture of microbes. The agent is formed by mixing the following compositions in percent by volume, 5-10% of photosynthetic bacterium fermentation liquid, 10-15% of bacillus subtilis fermentation liquid, 20-25% of plant lactobacillus fermentation liquid, 20-25% of microzyme fermentation liquid, 20-25% of actinomyces fermentation liquid, and 10-15% of enterococcus faecalis fermentation liquid. The six bacterial strains can be subjected to secondary enlarged culture under proper conditions of temperature, throughput, medium, acid-base value and culture time, and the culture processes are controlled in fermentation tanks free of infectious microbes. The efficient microbial soil repairing agent is produced by combining the high-density purely-cultured single-strain microbes.

Description

technical field [0001] The invention relates to a microbial soil restoration agent and a preparation method thereof, belonging to the technical field of microbial propagation and cultivation. Background technique [0002] With the development of agricultural planting, more and more chemical fertilizers, pesticides and other chemical substances are accumulated in the farmland, resulting in soil compaction and many diseases that directly affect the yield and quality of agricultural products. This problem has become an urgent problem for the planting industry to overcome. . The products used to restore soil on the market are mainly chemical ingredients, which are likely to cause secondary pollution after use. At present, EM bacteria are popularized in China, and the effect is not ideal, especially in the aspect of anti-cropping. The disadvantage of EM bacteria is mainly that the composition is unstable. Antagonism is formed, and only one strain of dominant bacteria grows up, ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16B09C1/10C12R1/125C12R1/25C12R1/01C12R1/865C12R1/465
Inventor 张进宋玉庄
Owner 康源绿洲生物科技(北京)有限公司
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