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Method for preparing cholesterol reducing eggnog fermenting drink by utilizing lactobacillus and microzyme which produce bile salt hydrolase

A bile salt hydrolysis enzyme and cholesterol-lowering technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, food preparation, etc., to achieve the effect of reducing cholesterol content

Active Publication Date: 2010-02-10
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are no relevant articles or patent reports about Kluyveromyces marxii high-efficiency cholesterol-lowering and high-production bile salt hydrolytic enzymes at home and abroad
However, there are no domestic and foreign literature reports and patent reports about the use of lactic acid bacteria and yeasts that produce bile salt hydrolase in the production of cholesterol-lowering egg-milk fermented beverages.

Method used

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  • Method for preparing cholesterol reducing eggnog fermenting drink by utilizing lactobacillus and microzyme which produce bile salt hydrolase
  • Method for preparing cholesterol reducing eggnog fermenting drink by utilizing lactobacillus and microzyme which produce bile salt hydrolase
  • Method for preparing cholesterol reducing eggnog fermenting drink by utilizing lactobacillus and microzyme which produce bile salt hydrolase

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Embodiment 1, Lactobacillus casei (Lactobacilluscasei) KL1 of high-efficiency cholesterol-lowering and high-production bile salt hydrolase, Lactococcus lactis subspecies (Lactococcus lactis subsp. identification

[0044] 1. Screening of lactic acid bacteria strains with high cholesterol-lowering efficiency and high production of bile salt hydrolase

[0045] Take 5mL of the filtrate of Zangling mushroom and pour it into 45mL sterile saline with glass beads, shake it fully for 30min to make a bacterial suspension, and then dilute it 10 times to 10 -4 ~10 -6 diluted bacterial solution. Inoculate the plate with the dilution pouring method, pour into the MRS selective medium containing calcium carbonate and natamycin, incubate at 37°C for 24 hours, pick a single colony with a dissolution circle and inoculate it in the MRS slant medium, and incubate at 37°C Cultivate for 24h. Gram staining, depending on the individual shape and purity. Inoculate in MRS liquid medium, enr...

Embodiment 2

[0059] Example 2. Screening and Identification of Kluyveromyces marxianus K1 and M3 Strains with High Efficiency in Lowering Cholesterol and High Production of Bile Salt Hydrolase

[0060] 1. Screening of high-efficiency cholesterol-lowering and high-production bile salt hydrolase yeast strains

[0061] Take 5mL of the filtrate of Zangling mushroom and pour it into 45mL sterile saline with glass beads, shake it fully for 30min to make a bacterial suspension, and then dilute it 10 times to 10 -4 ~10 -6 diluted bacterial solution. The plates were inoculated by the dilution pouring method, poured into the potato lactose selective medium containing antibiotics, and cultured at 28°C for 2-3 days. According to the colony characteristics of the yeast and the results of microscopic examination of the water immersion slides, the target colonies on the above plate were streaked and inoculated on the corresponding slant medium, and cultured at 28°C for 2-3 days. Transfer to inoculate ...

Embodiment 3

[0075] Example 3, using three strains of lactic acid bacteria with high bile salt hydrolytic enzyme production and a yeast strain two-step fermentation method to prepare a cholesterol-lowering egg-milk fermented beverage

[0076] 1. Preparation of lactic acid bacteria single starter

[0077] Put 10mL of skim milk into a test tube, sterilize at 0.07Mpa for 20min, cool to 37°C, take 2~3 rings with spiral inoculation loops, and inoculate Lactobacillus casei KL1 producing bile salt hydrolyzing enzyme and Lactococcus lactis subsp. KS4, a preserved strain of Streptococcus thermophilus Tx skim milk, was cultured at 37°C until the milk coagulated, and activated for 2 to 3 generations. Transfer the above-mentioned activated strains to 10ml sterile skim milk with 1%-2% inoculation amount, culture overnight at 37°C until the milk coagulates, then transfer to 200ml sterilized milk with 2%-3% inoculum amount Cultivate in a triangular flask at 37°C until the milk coagulates, which is a sin...

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Abstract

The invention relates to a method for preparing cholesterol reducing eggnog fermenting drink by utilizing lactobacillus and microzyme which produce bile salt hydrolase, belonging to a functional drinkwhich is prepared from the following ingredients: 15% of whole egg pulp, 35% of milk, 7% of saccharose, 0.3% of CMC-Na. and 0.2% of carrageenin, wherein the ratio of the raw materials (the whole eggplug and the milk) to water is 1:1 (W / V). The primary fermentation condition is described as following: streptococcus thermophilus Tx, Lactococcus lactis subsp.lactis KS4 and Lactobacillus casei KL1 are mixed in proportion of 1:1:1, the inoculum size is 3%, and then the mixture is fermented for 8h at the temperature of 43 DEG C. The secondary fermentation condition is described as following: Kluyveromyces marxianus M3 and K1 are compounded in proportion of 1:1 or the inoculum size of the single strain is 1%, and then the composite is fermented for 24-26 h at the temperature of 30 DEG C (mixture strain) or 32-34 DEG C; after ripening is carried out for 1 day at the temperature of 4 DEG C, the reducing rate of raw material cholesterol reaches as high as 89.3%. By utilizing the characteristics of strain high-yielding BSH, exopolysaccharide and the like, the invention has the efficiencies of immunological regulation, cholesterol reducing, tumor prevention and the like, and the problem of egg smell is not only solved, but also the content of raw material cholesterol is efficiently reduced through egg pulp proper sterilization and eggnog secondary fermentation. The eggnog drink preparedby the invention has simple fermentation process, short period and lower cost and is suitable for industrialized production.

Description

technical field [0001] The invention relates to three strains of lactic acid bacteria producing bile salt hydrolase (BSH), two strains of yeast producing bile salt hydrolase and their functional beverage production technology, which is suitable for cholesterol-lowering egg milk in fermented egg milk products Production of fermented beverages. Background technique [0002] Probiotics refer to a class of microorganisms that improve the microecological balance of specific parts of the host through intestinal colonization and have several other beneficial physiological functions. They can improve the ecological balance of intestinal microorganisms, control intestinal infection, reduce serum cholesterol levels, improve the utilization of lactose for people with lactose intolerance, and can stimulate specific or non-specific immune responses, and have the effect of immune enhancement. The live bacteria of probiotic products should have stable metabolism and strong activity, survi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L2/38A23L2/84A23L1/30C12N1/20C12N1/16C12R1/245C12R1/46C12R1/225C12R1/645A23L33/14
Inventor 刘慧钟思琼张红星贾慧徐振杰张嵩梁彦赵贵龙
Owner BEIJING UNIV OF AGRI
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