The invention provides special water-soluble fertilizer for aeroponics of climbing plants. Every 100 parts of the nutritional fertilizer is prepared from the following effective components: 0.5-1 part of a surfactant, 0.3-0.5 parts of a growth regulator, 0.5-1 part of bacillus, 0.3-0.5 parts of gamma-PGA, 13-16 parts of N, 4-17 parts of P2O5, 19-30 parts of K2O, 0.5-1.5 parts of Ca, 0.4-0.8 parts of Mg, 0.13 parts of Fe, 0.05 parts of Mn, 0.05 parts of Zn, 0.05 parts of B, 0.01 parts of Cu and 0.001 parts of Mo. The invention further provides a preparation method of the water-soluble fertilizer. The method comprises the following steps: (1) raw materials comprising N, P2O5 and K2O are broken into powder and mixed, and a first mixture is obtained; (2) raw materials comprising Ca, Mg, Fe, Mn, Zn, B, Cu and Mo are broken into powder and mixed, and a second mixture is obtained; (3) the second mixture, the surfactant, the growth regulator, the bacillus and the gamma-PGA are mixed, and a third mixture is obtained; (4) the first mixture and the third mixture are mixed, and the water-soluble fertilizer is obtained. The production process is simple, and the production cycle is short.