Method for synergistically degrading oil producing wastewater by using petroleum degrading bacteria

A technology for petroleum degrading bacteria and oil extraction wastewater, which is applied in mining wastewater treatment, chemical instruments and methods, biological water/sewage treatment, etc., and can solve the problems of low efficiency of oily wastewater and long wastewater treatment time.

Inactive Publication Date: 2014-05-21
CHANGZHOU UNIV
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Problems solved by technology

The ability to screen out highly efficient petroleum-degrading bacteria is of great significance to the treatment of oilfield wastewater and will...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Inoculate the purified single strains of Pseudomonas aeruginosa, Bacillus subtilis, Pseudomonas mendoza and Acinetobacter baumannii into beef extract peptone medium, pH 7.5, 35°C, 200r / min Cultivate on a shaking table for 18 hours to obtain the seed bacterial liquid;

[0033] (2) Prepare the domestication culture medium for enriching the bacterial liquid, the method is as follows:

[0034] Take the seed bacterial liquid and add it to the culture medium containing the crude oil sample, cultivate it on a shaker at pH 7.5, 33°C, and 150 r / min for 2 days, the volume content of the crude oil sample in the medium is 1.5%, and inoculate it into the fresh medium containing the crude oil sample; The above steps were repeated 4 times, and the volume content of the crude oil sample in the culture medium for each transfer was increased at a rate of 5‰, and the acclimatization culture medium was obtained after 8 days.

[0035] Wherein said culture medium is composition and part...

Embodiment 2

[0040] (1) Inoculate the purified single strains of Pseudomonas aeruginosa, Bacillus subtilis, Pseudomonas mendoza and Acinetobacter baumannii into beef extract peptone medium, pH 7.5, 35°C, 200r / min Cultivate on a shaking table for 18 hours to obtain the seed bacterial liquid;

[0041] (2) Prepare the domestication culture medium for enriching the bacterial liquid, the method is as follows:

[0042] Take the crude oil production fluid, add the corresponding carbon-free basic salt medium and seed bacteria solution to it, the volume ratio of crude oil production fluid and carbon-free basic salt medium is 1:5, pH 7.5, 33°C, 150r / min shaking Bed culture for 2 days; the culture solution obtained from the above culture was inoculated into the same fresh crude oil production fluid and culture medium, and cultured under the same conditions for 2 days, so that the enrichment culture was continued for four times, and the domestication culture solution was obtained after 8 days.

[004...

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PUM

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Abstract

The invention provides a method for synergistically degrading oil producing wastewater by using petroleum degrading bacteria. The method comprises the following steps: (1) inoculating purified single pseudomonas aeruginosa, bacillus subtilis, pseudomonas mendocina and acinetobacter baumannii strains into a beef extract-peptone medium, and culturing for 18 hours so as to obtain seed bacterium liquid; (2) preparing an acclimation culture solution of the seed bacterium liquid; (3) adding the prepared acclimation culture solution to a fermentation medium according to a single strain bacterium liquid isopyknic ratio, and culturing; (4) putting fermented bacterium liquid in an aeration tank so as to treat the oil producing wastewater. The composite high-efficiency oil removal bacterial strain can be used for degrading more than 94% of hydrocarbon compounds in the oil producing wastewater; the selected strains are respectively high in crude oil degradation rate and have synergistic effect; the combined degradation efficiency of several bacteria for crude oil is considerably higher than the simple superimposed effect of the single effect of the bacteria.

Description

technical field [0001] The invention relates to the technical field of sewage treatment, in particular to a method for synergistically degrading oil extraction wastewater by oil-removing bacteria. Background technique [0002] In the initial stage of oilfield development, usually after the oil well is drilled, the original formation energy such as natural water flooding, elastic energy flooding, solution gas flooding and gravity flooding can lift the formation crude oil to the surface through the oil flow channel, which is self-spraying. The way of extracting oil is called primary oil recovery. The water content of primary oil recovery fluid is very low, about 5-10%. The primary oil recovery causes the energy of the original formation to decline rapidly, and it is difficult to maintain the crude oil recovery method by relying on the energy of the original formation. At this time, it is necessary to inject water or gas into the underground oil layer to supplement the energy ...

Claims

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Application Information

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IPC IPC(8): C02F3/34C02F103/10
Inventor 冯俊生杨怀成常杰云刘兆跃秦学成陈皓程汉东
Owner CHANGZHOU UNIV
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