Method for synergistically degrading oil producing wastewater by using petroleum degrading bacteria
A technology for petroleum degrading bacteria and oil extraction wastewater, which is applied in mining wastewater treatment, chemical instruments and methods, biological water/sewage treatment, etc., and can solve the problems of low efficiency of oily wastewater and long wastewater treatment time.
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Embodiment 1
[0032] (1) Inoculate the purified single strains of Pseudomonas aeruginosa, Bacillus subtilis, Pseudomonas mendoza and Acinetobacter baumannii into beef extract peptone medium, pH 7.5, 35°C, 200r / min Cultivate on a shaking table for 18 hours to obtain the seed bacterial liquid;
[0033] (2) Prepare the domestication culture medium for enriching the bacterial liquid, the method is as follows:
[0034] Take the seed bacterial liquid and add it to the culture medium containing the crude oil sample, cultivate it on a shaker at pH 7.5, 33°C, and 150 r / min for 2 days, the volume content of the crude oil sample in the medium is 1.5%, and inoculate it into the fresh medium containing the crude oil sample; The above steps were repeated 4 times, and the volume content of the crude oil sample in the culture medium for each transfer was increased at a rate of 5‰, and the acclimatization culture medium was obtained after 8 days.
[0035] Wherein said culture medium is composition and part...
Embodiment 2
[0040] (1) Inoculate the purified single strains of Pseudomonas aeruginosa, Bacillus subtilis, Pseudomonas mendoza and Acinetobacter baumannii into beef extract peptone medium, pH 7.5, 35°C, 200r / min Cultivate on a shaking table for 18 hours to obtain the seed bacterial liquid;
[0041] (2) Prepare the domestication culture medium for enriching the bacterial liquid, the method is as follows:
[0042] Take the crude oil production fluid, add the corresponding carbon-free basic salt medium and seed bacteria solution to it, the volume ratio of crude oil production fluid and carbon-free basic salt medium is 1:5, pH 7.5, 33°C, 150r / min shaking Bed culture for 2 days; the culture solution obtained from the above culture was inoculated into the same fresh crude oil production fluid and culture medium, and cultured under the same conditions for 2 days, so that the enrichment culture was continued for four times, and the domestication culture solution was obtained after 8 days.
[004...
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