Polypeptide for inhibiting hepatitis C virus infected cell and application thereof

A hepatitis C virus, cell technology, applied in the direction of antiviral agents, peptides, peptide/protein components, etc., can solve the problems of side effects, lack of inhibitors, and difficulties in vaccine research.

Active Publication Date: 2012-03-21
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Due to the high variability of the virus itself, HCV has caused great difficulties in vaccine research
The current treatment of HCV infection is limited to the use of interferon (interferon, IFN) alone, or combined with broad-spectrum antiviral drug ribavirin (ribavirin), but the treatment effect is not good, there are certain side effects, and different types of Responsiveness to IFN varies greatly
For many years, researchers have been looking for effective targets to prevent and treat infections, but no specific and effective inhibitors have been obtained, and the effect of use is still in the preliminary evaluation stage of experimental or clinical research

Method used

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  • Polypeptide for inhibiting hepatitis C virus infected cell and application thereof
  • Polypeptide for inhibiting hepatitis C virus infected cell and application thereof
  • Polypeptide for inhibiting hepatitis C virus infected cell and application thereof

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Embodiment Construction

[0020] The present invention will be further described in detail below in conjunction with specific embodiments, which are explanations of the present invention rather than limitations.

[0021] 1. Construction and expression of HCV E2 protein

[0022] 1.1 Construction of E2 recombinant plasmid by molecular cloning method

[0023] The gene of the envelope glycoprotein E2 of HCV (belonging to 1a genotype) was amplified by PCR, and EcoRI and HindIII enzyme cutting sites were designed at the 5' end and 3' end of the upstream and downstream primers respectively, and the amplified clone product was The constructed restriction site was cloned into the pET28a vector (purchased from Novagen, Cat. No. 69864-3), and the recombinant vector obtained was named pET28a-E2661. The specific construction method was as follows:

[0024] First, the HCV E2661 gene coding region fragment was amplified by PCR method, EcoRI and HindIII restriction sites were designed at both ends of the primers, and...

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Abstract

The invention discloses polypeptide for inhibiting a hepatitis C virus infected cell and an application thereof. The sequence of the polypeptide is shown as SEQ ID NO: 1 or SEQ ID NO: 2. Binding adsorption can be realized for the polypeptide and HCV (hepatitis C virus) expressed E2 protein so as to block invasion or transfection of the HCV for cells. The inhibition of the polypeptides TD1 and TD2for HCV infected cells can be detected through a HCV pseudovirus (HCVpp) and HCV RNA (ribonucleic acid) transfected cell cultivation (HCVcc) system, and results show that TD1 and TD2 have certain inhibitory effects on HCVpp and HCVcc infected cells and can be used as an adsorption inhibitor or blocker for the HCV.

Description

technical field [0001] The invention belongs to the technical field of preventing HCV infection, and relates to a polypeptide for inhibiting hepatitis C virus from infecting cells and an application thereof. Background technique [0002] HCV (hepatitis C virus) is a flavivirus, a single-stranded positive-sense RNA virus, composed of structural proteins (Core, E1, E2) and non-structural proteins (NS2-NS5B). HCV is the pathogen of hepatitis C. About 75% of patients develop chronic hepatitis after acute infection, and about 20% of them end up with liver cirrhosis or liver failure, and may further develop into liver cancer. At present, there are 170 million infected people in the world, and about 40 million in my country. HCV infection has become one of the global social public health problems. [0003] Due to the high variability of the virus itself, HCV has caused great difficulties in vaccine research. The current treatment of HCV infection is limited to the use of interfer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06A61K38/08A61P1/16A61P31/14
Inventor 吕欣尹文张芳琳李玉成姚敏贾战生黄小军张建敏雷迎峰杨敬兰海云
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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