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Rhizoma polygonati beta-galactan, preparation method thereof, and application to anti-inflammatory aspect

A technology of galactan and Polygonatum, applied in application, anti-inflammatory agent, food preparation, etc., can solve problems such as few, single-component structure-activity relationship, pharmacological activity mechanism is not clear, unfavorable application and development of Polygonatum polysaccharide, etc.

Active Publication Date: 2015-12-16
SHANGHAI JAHWA UNITED +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there are not many reports on the single uniform component in Polygonatum Polysaccharides, and the structure-activity relationship and pharmacological activity mechanism of various single components are not clear.
This situation is not conducive to the application and development of Polygonatum polysaccharide

Method used

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  • Rhizoma polygonati beta-galactan, preparation method thereof, and application to anti-inflammatory aspect
  • Rhizoma polygonati beta-galactan, preparation method thereof, and application to anti-inflammatory aspect
  • Rhizoma polygonati beta-galactan, preparation method thereof, and application to anti-inflammatory aspect

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1: Extraction of Polygonatum β-galactan

[0053] Get 2.0kg of Rhizoma Polygonatum medicinal material (place of origin: Anhui, purchased from Shanghai Huayu Pharmaceutical Co., Ltd.), add organic solvents (such as ethanol, acetone, chloroform, etc.), soak at room temperature, once every five days, twice altogether, to remove fat-soluble small molecular. Pour off the supernatant, put the solid in a ventilated place to dry, and then extract it with hot water, add about 20-40L each time, the extraction time is 5-7 hours, use the phenol-sulfuric acid method to detect the sugar content of the extract, A total of 6 extractions were performed until the sugar reaction was not obvious. Each extract was filtered and combined, heated and concentrated to a small volume, dialyzed against flowing water for two days, the dialyzed liquid was concentrated and then centrifuged, 1:1 (v / v) was added with 30% trichloroacetic acid solution to remove protein, and the protein was re...

Embodiment 2

[0063] Embodiment 2: Polygonatum β-galactan HJWOS300-2 inhibits NF-κB activity experiment

[0064] 1.1 Construction of HEK293 / NF-κB-luc stable strain

[0065] The cell line was constructed by Dr. Fang Jianping from the laboratory of Ding Kan, Shanghai Institute of Materia Medica (Journal of Agriculture and Food Chemistry, 61(47): 11400-9, 2013, Glycoconjugate journal, 2012, 29, 389). Specific steps are as follows:

[0066] Convert HEK293 to 3×10 5 cells / ml were inoculated in 12-well plate, 1ml / well. After culturing overnight, the density reached between 70% and 90%, and transfection was performed according to the instructions of Lipofectamine2000 (Invitrogen). After overnight culture, the cells were subcultured at a ratio of 1:60, and transferred to a 100 mm cell culture dish. After 24 hours, 800 μg / ml of G418 (Merk, USA) was added to continue the culture. Change the medium once a week and add new G418. After about 5-6 weeks, a single colony formed, jumped out with a pipe...

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Abstract

The invention discloses rhizoma polygonati beta-galactan HJW0S300-2, which has a structural formula as shown in the description. The rhizoma polygonati beta-galactan HJW0S300-2 has the molecular weight in a range of 8-15 KDa, and the average molecular weight (Mw) of 10 KDa. The invention further relates to a preparation method of rhizoma polygonati beta-galactan HJW0S300-2, and an application of rhizoma polygonati beta-galactan HJW0S300-2 to an anti-inflammatory aspect.

Description

technical field [0001] The invention relates to polysaccharides extracted from Chinese herbal medicines, more specifically, to a β-galactan HJWOS300-2 extracted, separated and purified from Rhizoma Polygonatum, its preparation method and application. The invention relates to the anti-inflammatory effect of β-galactan HJWOS300-2 separated and purified from Chinese herbal medicine Polygonatum. It specifically relates to the use of the polysaccharide in preparing medicines, health care products and cosmetics related to alleviating or treating inflammation. Background technique [0002] Polygonatum sibiricum, also known as tiger ginger and chicken head ginger, is the dry tuber of various plants of the genus Polygonatum in the family Liliaceae. Polygonatum has the effects of nourishing the kidney and nourishing yin, nourishing the middle and replenishing qi, and prolonging life. Mainly distributed in Gansu and Shaanxi. Pharmacological studies have shown that Polygonatum has an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K8/73A61K31/715A61P29/00A23L1/09
Inventor 王滢张庆丁侃赵亚魏少敏吴越祝乐冯冰
Owner SHANGHAI JAHWA UNITED
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