Method for simultaneously extracting arabinogalactan and dihydroquercetin from dahurian larch

A technology of arabinogalactan and dihydroquercetin, which is applied in the field of simultaneous extraction of arabinogalactan and dihydroquercetin, can solve the problems of no enrichment and purification, many times of crystallization operations, and low purity of crude products, etc. problems, to achieve the effect of convenient industrial scale production, simple operation and high yield

Inactive Publication Date: 2015-01-21
NORTHEAST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the following defects: although wood powder is used as raw material for sufficient extraction, the solvent consumption is large, and the residue is difficult to recycle; arabinogalactan and dihydroquercetin cannot be extracted in one step, which not only increases the cost but also lengthens The production cycle is shortened; without enrichment and purification, the purity of the crude product is low, so that the number of crystallization operations is more

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 100.0g of 9.83% water-containing larch, crushed, add 1000mL 40% ethanol solution, 250W ultrasonic extraction for 60min, cool and filter, the concentration of dihydroquercetin in the filtrate is about 0.013mg / mL, add absolute ethanol to the volume of ethanol in the solution The fraction was 80%, centrifuged at 10,000 r / min for 5 min, and the precipitate was freeze-dried to obtain 14.34 g of arabinogalactan crude extract. Concentrate the supernatant under reduced pressure until there is no alcohol smell, and heat the water to fully dissolve it. At this time, the concentration of dihydroquercetin in the water phase is about 0.055 mg / mL. The AB-8 macroporous adsorption resin is used to enrich the two in the water phase. Hydroquercetin, eluted with 80% ethanol solution, recovered ethanol solution to obtain liquid viscous 2.11g, the content of dihydroquercetin was 30.8%, heated water fully dissolved, 4℃ refrigerated crystallization, recrystallization 3 times to obtain Dihydroq...

Embodiment 2

[0025] 10.0g of larch with 9.53% water content is crushed, add 100mL of 20% ethanol solution, 450W ultrasonic extraction for 45min, cooling and filtering, add 100mL 20% ethanol solution, 450W ultrasonic extraction for 45min to the raw material, cooling and filtering the combined filtrate, the filtrate contains dihydrogen The concentration of quercetin is about 0.014 mg / mL, and absolute ethanol is added to make the ethanol volume fraction in the solution 70%, centrifuged at 10,000 r / min for 5 min, and the precipitate is freeze-dried to obtain 1.44 g of arabinogalactan crude extract. Concentrate the supernatant under reduced pressure until there is no alcohol, and heat the water to fully dissolve it. At this time, the concentration of dihydroquercetin in the water phase is about 0.057 mg / mL. The AB-8 macroporous adsorption resin is used to enrich the water phase. Hydroquercetin was eluted with 80% ethanol solution, and the ethanol solution was recovered to obtain 0.261g of liquid ...

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Abstract

The invention discloses a method for extracting arabinogalactan and dihydroquercetin from dahurian larch. The method is characterized in that dahurian larch is taken as a raw material and is subjected to supersonic extraction with an ethanol solution; solid and liquid are separated from each other through filtering, so that an extracting solution is obtained; absolute ethyl alcohol is fed into the extracting solution till polysaccharide is sufficiently settled; the polysaccharide is subjected to high-speed centrifugation; precipitation, refrigeration and drying are performed, so that dahurian larch arabinogalactan crude extract is obtained; ethyl alcohol in supernate is concreted till solid matter is obtained; the solid matter is dissolved through hot water and then is enriched through macroporous adsorption resin; elution and recrystallization are performed, so that dihydroquercetin extract is obtained. The method has the main characteristics that the raw material is not required to be dried and directly used, the energy consumption is low, by adjusting the content of ethanol in the ethanol solution for extracting, arabinogalactan and dihydroquercetin are simultaneously extracted, the production cost is low, energy consumption is reduced, the yield of products is high, the process of purification performed by the macroporous adsorption resin is pollution-free, all solvents can be recycled, and the method is convenient for industrial scale production.

Description

Technical Field: [0001] The invention relates to the field of natural product chemistry, in particular to a method for simultaneously extracting arabinogalactan and dihydroquercetin from larch. Background technique: [0002] Arabinogalactan is a kind of long, highly branched hemicellulose composed of arabinose and galactose. Its main chain is galactan, and the branches are mainly arabinose side chains, passing β-1,3 The bond or β-1,6 bond is connected to the galactose chain. The content in larch is 10%-30%. Arabinogalactan is used in food and daily chemical industries as a mixture, emulsifier, stabilizer, sweetener, flavoring agent, and printing and mining industries. In recent years, arabinogalactan in larch wood has a unique role in the treatment of difficult and common diseases and a wide range of biological activities, without any toxic effects (it has been certified by the US FDA in 1974 and can be used in food). Administration, its medicine and health care functions are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/14C07D311/32C07D311/40
Inventor 马春慧祖元刚李坚杨磊李伟
Owner NORTHEAST FORESTRY UNIVERSITY
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