Transcription factor-mediated promotion of direct somatic embryogenesis

A technology of transcription factors and cells, applied in the field of plant micropropagation, can solve the problems of high cost, large amount of chemicals, and long time.

Inactive Publication Date: 2021-10-29
比贝婷作物科学有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0025] However, this method takes a long time to obtain somatic embryos and regenerated plants, and may produce a large number of abnormal embryos
In addition, due to the reliance on callus formation, there is a high potential for somaclonal variation among regenerated plants
The technology remains costly given the lengthy process and the large amount of chemicals required

Method used

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  • Transcription factor-mediated promotion of direct somatic embryogenesis
  • Transcription factor-mediated promotion of direct somatic embryogenesis
  • Transcription factor-mediated promotion of direct somatic embryogenesis

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Embodiment Construction

[0065] While exemplary embodiments are described below, the present invention is only limited by the claims. Thus, the claims are hereby expressly incorporated into the Detailed Description, where each claim, and each combination of claims allowed by claim dependency, each constitutes a various embodiment of the present invention.

[0066] In the claims, the word "comprising" or "comprising" is not limited to the features, elements or steps stated thereafter, nor does it exclude other features, elements or steps. This term indicates the presence of the stated feature, but does not preclude the presence or addition of one or more features.

[0067] Various specific details are given in the detailed description herein. Embodiments of the invention may be practiced without these specific details. Furthermore, well-known features, elements and / or steps may not necessarily be described in detail herein for clarity and conciseness.

[0068] The implementation of the present inven...

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Abstract

A recombinant protein for use in a liquid culture medium for photo-autotrophic micropropagation of Cannabis Sativa L. is disclosed. The recombinant protein comprises a fusion of a growth induction part and a uptake enhancement part. The growth induction part comprises an Arabinogalactan protein and / or a plant transcription factor associated with plant growth and development. The uptake enhancement part comprises a cell penetrating peptide sequence and a nuclear localization signal encoded by the peptide sequence SEQ ID NO 1.

Description

technical field [0001] The invention relates to the field of plant micropropagation, that is, artificial plant propagation through tissue culture technology. More specifically, the present invention relates to methods of micropropagation of cannabis, recombinant proteins, cell culture media comprising the recombinant proteins and methods of producing the recombinant proteins. Background technique [0002] Vegetative propagation of plants, for example by vegetative propagation and / or tissue culture, can be used in plant culture processes for agricultural and / or biochemical purposes. For example, cloning can reduce variation among plants, allowing better control over the chemical properties of biomass products. In addition, single plants or small numbers of plants can be selected for large-scale trait replication in the next generation of plants based on preferential traits. [0003] In vegetative propagation, clonal copies of the parent plant can be obtained by taking cutti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C07K14/415C12N5/00C12N5/04
CPCC07K14/415A01H4/005C12N15/8262A01H4/002C07K2319/033C07K2319/09
Inventor J·霍芬克J·托池
Owner 比贝婷作物科学有限公司
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