Method for producing sugar liquid
A manufacturing method and technology of sugar solution, applied in the direction of sugar production, sugar juice purification, chemical instruments and methods, etc., can solve the problem of cost, remove objects limited to hydrophobic compounds, remove low efficiency and other problems, and achieve the goal of improving efficiency Effect
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[0144] Hereinafter, in order to demonstrate in more detail about the manufacturing method of the sugar liquid of this invention, an Example is given and demonstrated. However, the present invention is not limited to these Examples.
reference example 1
[0145] (Reference example 1) Analysis method of monosaccharide concentration
[0146] The monosaccharide concentration contained in the obtained aqueous sugar solution was quantified by comparison with a standard under the HPLC conditions shown below.
[0147] Column: Luna NH 2 (manufactured by Phenomenex Corporation)
[0148] Mobile phase: ultrapure water: acetonitrile = 25:75 (flow rate 0.6mL / min)
[0149] Reaction solution: no
[0150] Detection method: RI (differential refractive index)
[0151] Temperature: 30°C.
reference example 2
[0152] (Reference example 2) Analytical method of fermentation inhibitor concentration
[0153] The furan-based fermentation inhibitors (HMF, furfural) and phenolic fermentation inhibitors (vanillin, acetylvanillin, syringic acid, levulinic acid, 4-hydroxybenzoic acid) contained in the sugar solution were tested under the HPLC conditions shown below Quantification is performed by comparison with standards.
[0154] Column: Synergi HidroRP 4.6mm×250mm (manufactured by Phenomenex)
[0155] Mobile phase: Acetonitrile-0.1%H 3 PO 4 (flow rate 1.0mL / min)
[0156] Detection method: UV (283nm)
[0157] Temperature: 40°C.
[0158] The organic acid-based fermentation inhibitors (acetic acid, formic acid) contained in the sugar solution were quantified by comparison with standard samples under the HPLC conditions shown below.
[0159] Column: Shim-Pack SPR-H and Shim-Pack SCR101H (manufactured by Shimadzu Corporation) in series
[0160] Mobile phase: 5mM p-toluenesulfonic acid (fl...
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