Polynucleotide for identifying male and female ginkgo strains and application thereof

A technology of polynucleotides and nucleotides, which is applied to the identification of male and female ginkgo polynucleotides and its application fields, which can solve the problem that plant sex has no practical significance, and achieve the effects of rapid detection, good repeatability, and stable and accurate results

Active Publication Date: 2019-09-03
AGRI GENOMICS INST CHINESE ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, according to karyotype observation, there is no conclusion on whether the sex determination mechanism of ginkgo is XY type or ZW type, and it has no practical significance to rely on karyotype observation to determine plant sex

Method used

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  • Polynucleotide for identifying male and female ginkgo strains and application thereof
  • Polynucleotide for identifying male and female ginkgo strains and application thereof
  • Polynucleotide for identifying male and female ginkgo strains and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0067] 1. Synthesis of primers

[0068] By resequencing the male and female plant populations, K-mer distribution analysis was used to obtain sex-specific K-mers. On this basis, male-specific sequencing fragments were obtained, and male-specific fragments were obtained through assembly. These fragments are characterized in that they only exist in male individuals, and this method selects one of the contigs, named Contig1634, its specific sequence is shown in SEQ ID NO: 5, and its coding sequence is shown in SEQ ID NO: 6. Contig1634 is compared with the reference genome sequence (GIGADB (doi:10.5524 / 100209), from female plants) as follows figure 2 shown. from figure 2 It can be seen from the figure that there are many differences between Contig1634 and the reference genome, and the difference positions are evenly distributed in the whole sequence. The short fragment on Contig1634 can be used to identify the sequence.

[0069] Primers designed with the Contig1634 sequence w...

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Abstract

The invention provides a polynucleotide for identifying male and female ginkgo strains. The polynucleotide comprises or consists of: 1) a nucleotide sequence shown as SEQ ID NO: 5; or 2) a complement,degenerate or homologous sequence of the sequence shown as SEQ ID NO: 5; or 3) a polynucleotide which hybridizes to the nucleotide sequence shown in SEQ ID NO: 5 under stringent conditions or a complement thereof; 4) a coding sequence of any of the sequences 1) to 3); or 5) a nucleotide sequence identical to a nucleotide of 10 bp or more in any of the sequences of sequences 1) to 4). The polynucleotide provided by the present invention is unique among male ginkgo plants, uses the same to identify male and female plants of ginkgo, has the advantages of stable and accurate results, good reproducibility and rapid detection, and provides a basis for the identification of male and female ginkgo by molecular markers.

Description

technical field [0001] The invention relates to the identification field of male and female ginkgo plants, in particular to a polynucleotide for identifying male and female ginkgo plants and its application. Background technique [0002] Ginkgo biloba L. integrates medicinal, edible, material and ornamental uses, and has special economic value and important scientific research value. In recent years, the ginkgo industry in my country has developed rapidly, and the planting scale has gradually expanded, especially in landscaping projects. Ginkgo biloba is a dioecious plant, and it takes 15-20 years after the seedlings are planted to bloom and bear fruit, and separate male and female. But because ginkgo seed has a kind of special stink in ripe stage, pollutes urban street after the fallen fruit rots, so only need male plant as urban greening tree species, do not need to be the female plant for the purpose of producing ginkgo. Scholars at home and abroad have conducted resear...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12Q1/6895C12N15/11
CPCC07K14/415C12Q1/6895C12Q2600/13C12Q2600/158C12Q1/686A01H5/12A01H7/00A01H1/045C12Q1/6844
Inventor 闫建斌廖庆刚苟君波杜然黄三文
Owner AGRI GENOMICS INST CHINESE ACADEMY OF AGRI SCI
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