39 results about "Disodium Fluorescein" patented technology

Apparatus and method for measuring the fluorescence of nanodrop liquid samples is described in which the sample is held by surface tension between two anvil surfaces. Each anvil surface has an embedded optical fiber with its end finished flush with the surface in the containment area wetted by the sample with the fiber in line. Sample excitation is provided from the side of the sample remote from the containment area. By selection of the fiber transmission numeric aperture the impact of exciting and ambient light on the measurement is minimized. A method of virtual filtering is taught in which any ambient or exciting light that does impinge on the measuring sensor is corrected by subtracting a scaled representation of the source from the measurement. The method and apparatus is capable of detecting 1 femptomole of sodium fluorescein in 1 microliter of TE buffer.

The invention discloses an assay method of a migration path of groundwater by using a tracer agent. The assay method includes the following steps that a tracer area is determined and a ground map of the tracer area is drawn; the type of the tracer agent is determined; the delivery quantity of the tracer agent is determined; a delivery well and monitoring wells are selected and distributed; delivery and sampling of the tracer agent are carried out; detection of a tracer sample is carried out; a result of the groundwater migration path is summarized; and result interpretation of the groundwatermigration path is carried out. According to the assay method of the migration path of the groundwater by using the tracer agent, an assay method of a sodium fluorescein tracer agent is adopted, a time-concentration relation curve is drawn, according to the time-concentration relation curve and the straight line distance between the delivery well and the monitoring wells, the groundwater average velocity and the groundwater flow direction of the delivery well and the monitoring wells are measured, then hydraulic power relation conditions of the groundwater in and out of the tracer area are found out, the groundwater flow rate is determined, and the main direction of the groundwater flow direction is determined.

The invention belongs to the technical field of film preparation, and provides a light conversion plastic agricultural film with long service life and a preparation method thereof. The method comprises the following steps: stirring and dissolving an alcohol solution, stearic acid and liquid glass, and adding sodium fluorescein; performing dispersion, oscillation, suction filtration and vacuum drying to prepare stearic acid and liquid glass coated sodium fluorescein; mixing a film base material, thermoplastic starch, a light stabilizer, an antioxidant, an antifogging agent and a lubricating agent, extruding and performing blow molding to obtain the light conversion plastic agricultural film with long service life. Compared with a traditional method, the method has the advantages that a compact protective layer is formed by using stearic acid and liquid glass coated sodium fluorescein, oxygenolysis of sodium fluorescein can be effectively prevented, and the light conversion plastic agricultural film has relatively light-heat performance, good light conversion effect and long service life; and the preparation process is simple in operation and relatively low in cost, and has a wide application range.

The invention relates to a method for measuring the total antioxidant capacity of small berries, provides an ORAC detection kit suitable for the determination of the total antioxidant capacity of small berries and a detection method thereof, and belongs to the technical field of activity detection and determination. The kit includes: antioxidant standard substance Trolox storage reagent, fluorescent substance fluorescein sodium storage reagent, free radical generator AAPH reagent, phosphate buffer, and fluorescent microwell reaction plate, which is made of a plastic or hard paper material Boxes are packed together to make up. The ORAC detection method for the total antioxidant capacity of small berries comprises the following steps: small berry sample pretreatment; detection with reagent kits; and analysis of detection results. By mixing the sample and the reagent in a certain volume ratio, the fluorescence microplate analyzer is used to detect the change of the fluorescence intensity of the complex, thereby measuring and calculating the total antioxidant capacity of the small berry. The invention can provide a complete set of total antioxidant capacity test of small berries, has high sensitivity and good accuracy, and is convenient for popularization and application.

The invention discloses a full-automatic configuration and release control device of a rhodamine B fluorescent tracer and an operation method. The full-automatic configuration and release control device comprises a water supplying device, an integrated three-chamber combined box body, a central control device and an online real-time monitoring device, wherein the integrated three-room combined boxbody comprises a premixing chamber, a mixing chamber and a storage chamber which are communicated in sequence; the premixing chamber is used for storing dry powder of three tracers such as rhodamine,sodium fluorescein and tinopal; the mixing chamber comprises a temperature sensor, a stirring rod and a cooling base, and the temperature sensor is arranged at the lower part of the mixing chamber; the stirring rod is used for stirring solution to be mixed; the cooling base is positioned at the bottom part of the premixing chamber, the temperature sensor is connected with the central control device and the central control device controls the cooling base to realize temperature control in the mixing chamber; the storage chamber is used for storing prepared solution. The online real-time monitoring device is used for monitoring various parameters of the prepared solution, transmitting data to the central control device and retaining historical data in time.

The invention discloses a preparation method for modified graphene. The preparation method comprises the following steps: preparing a graphene oxide/aqueous dispersion with a concentration of 0.01 to 3.0 mg/mL; adding sodium fluorescein into the graphene oxide/aqueous dispersion, and carrying out stirring; then adding an alkali solution and adjusting a pH value of a system to be 9 to 10; adding a chemical reducing agent, carrying out a reaction at 60 to 95 DEG C for 10 to 100 hours, and carrying out cooling to a room temperature; and subjecting the system to vacuum filtering or centrifuging, then carrying out washing with ethanol and deionized water to nearly neutral or neutral, and carrying out drying at 40 to 80 DEG C until a weight is constant so as to obtain the modified graphene. The preparation method provided by the invention is favorable for increasing and improving dispersibility of graphene in water and an organic solvent; meanwhile, the preparation method is simple, has mile condition, and is easy to operate and control.

The invention discloses an engine coolant and a preparing method thereof. The engine coolant is prepared from, by weight, 500-700 parts of water, 40-60 parts of sodium formate, 5-15 parts of borax, 0.2-1.5 parts of potassium molybdate, 0.5-1.5 parts of octanedioic acid, 1-10 parts of sodium caprate, 0.5-1.5 parts of sodium nitrate, 0.5-1.5 parts of benzotriazole, 1-3 parts of sodium hydroxide, 1-3 parts of sodium metasilicate nonahydrate, 200-400 parts of 1,2-propylene glycol, 50-100 parts of glycerine, 0.001-0.008 part of sodium fluorescein, 0.01-0.08 part of simethicone, 0.05-0.15 part of lanthanum chloride, 0.05-0.15 part of chelating agent and 0.1-0.5 part of silicate stabilizer. The engine coolant is high in stability, freezing resistance, rust resistance and scaling resistance, capable of avoiding pipeline blocking, effectively preventing corrosion to ferrous metal, tin, copper and copper alloy, aluminum and aluminum alloy, overcoming the technical defect of traditional water-containing cooling liquid that boiling occurs easily, facilitating full-load and high-load operation of an engine and ensuring that a vehicle can travel safely in severe environments such as highlands, deserts and cold areas, and especially suitable for military vehicles, high-end vehicles and high-power heavy-load trucks and passenger cars.

The invention belongs to the technical field of antifreeze fluid and discloses an automotive antifreeze fluid and a preparation method thereof. The automotive antifreeze fluid is prepared from, by mass, 400.0-600.0%o of polyalcohol, 1.0-5.0%o of acrylic acid-acrylate-sulfonate ternary copolymer, 1.0-5.0%o of polyoxyethylene polyoxypropylene segmented copolymer, 5.0-10.0%o of organic amine, 0.5-5.0%o of benzotriazole, 0.5-5.0%o of accelerants, 0.5-5.0%o of sodium hydroxide, 0.5-5.0%o of sodium nitrate, 0.5-5.0%o of sodium metasilicate, 2.0-8.0%o of organic acid, 0.005-0.5%o of sodium fluorescein, 0.005-0.5%o of turquoise blue pigment, 0.005-0.5%o of an anti-foaming agent and the balance deionized water. The automotive antifreeze fluid has advantages of low freezing point, high boiling point, excellent anti-freezing and anti-boiling performances and great corrosion resistance and anti-scaling performances.

The invention provides a method for testing the sealing integrity of a medicine packaging container by an improved color water intrusion method, and particularly relates to the technical field of sample pretreatment of medicines. The method comprises the steps of preparing a 1% sodium fluorescein solution, and putting the 1% sodium fluorescein solution into a sealing cavity of a sealing tester; inverting a prepared positive sample, submerging an inserted capillary tube by sterile powder, keeping the capillary tube immersed in a dye solution, and tightly covering the sealing cavity; and takingout the inverted positive sample after the invasion procedure is finished; adopting a sodium hydroxide solution with a concentration of 1 [mu] g/mL as a diluent; taking sterile powder for injection, which is not subjected to capillary insertion and color water invasion, fully redissolving the sterile powder by using the diluent, transferring the sterile powder into a clean centrifuge tube, and fixing the volume to 20mL; after the invasion procedure is finished, fully redissolving each bottle by using the diluent, transferring into a clean centrifuge tube, and fixing the volume to 20mL; and observing the dye invasion condition by naked eyes. According to the invention, the sensitivity of naked eye observation and detection can be significantly improved, the specificity is high, the anti-interference ability is strong, the detection efficiency is high, and the labor cost is saved.

The invention relates to a dyeing method of a digestive system of amblyseius tsugawai. The dyeing method is characterized by comprising the following steps of: in the conditions that the temperature is 25-30 DEG C and the humidity is 75-90%, pouring 0.05-5% of sodium fluorescein or an azaleine solution into a culture dish; then shearing a stretched parafilm to a circle, the diameter of which is smaller than the culture dish; then paving the sheared parafilm on the surface layer of the sodium fluorescein or the azaleine solution in the culture dish, wherein the parafilm floats on the solution of the culture dish; and finally, placing the amblyseius tsugawai starved for 0-72 hours on the parafilm to observe the digestive system of amblyseius tsugawai under a microscope within 1-3 hours. The dyeing method provided by the invention is simple to operate, and can provide technical reference to observe the digestive tract and the whole digestive system of the amblyseius tsugawai.

The invention provides a bacillus subtilis synergist and application thereof for preparing bacillus subtilis fungicide, and belongs to the technical field of microbial preparation. The bacillus subtilis synergist is prepared from, by mass, 0.5-1.5 parts of xanthan gum, 1-3 parts of sodium fluorescein, 1-3 parts of a fluorescent brightener CBS-X, 1-3 parts of butylated hydroxytoluene, 6-9 parts ofsaccharose and 2-3 parts of potassium nitrate, wherein the xanthan gum, the sodium fluorescein and the fluorescent brightener CBS-X can achieve the good anti-ultraviolet protection effect on bacillussubtilis; the butylated hydroxytoluene can achieve the good anti-oxidation effect on the bacillus subtilis; the saccharose and the potassium nitrate can achieve the obvious synergism on the bacteriostatic activity of the bacillus subtilis. The ingredients can have the good biologic compatibility with the bacillus subtilis.

The invention relates to a cornea stainer. The cornea stainer comprises a carrier and a packaging bag. Cotton yarn or a cotton thread is adopted as the carrier, dye A is adsorbed on the carrier, and the dye A is sodium fluorescein. The carrier is placed in the packaging bag. Preferably, dye B is further adsorbed on the carrier, and the dye B is methyl blue or placenta blue or lissamine green. Morepreferably, the mass ratio of the dye A to the dye B is 1:1. According to the cornea stainer, the cotton yarn or the cotton thread replaces filter paper, and the defects that the dyeing effect of thefilter paper is poor, and the dyeing dosage carried by the carrier is small, specially the defect that when two or more dyeing agents are used for mixed dyeing, the dyeing effect of the filter paperis poor.

A composition and method for applying the composition topically to an eye for improving corneal and surface hydration and reducing blue light neuro-stimulation, especially during the several hours preceding sleep. In one embodiment, the composition combines an artificial tear with fluorescein sodium. The artificial tear may include carboxymehylcellulose sodium, polyvinyl alcohol, hydroxypropyl cellulose and glycerin. In another embodiment, the composition comprises synthetic human melanin combined with fluorescein sodium in an aqueous solution to reduce ocular UV light and HEV light exposure.

The invention relates to a PEG nuclear-crosslinked star high-molecular nano contrast agent which is a star polymer having a PEG core prepared by bonding fluorescent groups. The invention also relatesto application of the PEG nuclear-crosslinked star high-molecular nano contrast agent in the preparation of fundal vascular contrast media. Part of patients intravenously injected with fundal vascularcontrast media clinically applied at present, such as sodium fluorescein or indocyanine green, experience adverse effects; these patients cannot take complete contrast examination. Compared with theprior contrast media, S-PPEGMA10-ICG herein has the advantages of low injection concentration and better choroidal development clarity; the side effects of prior drugs can be alleviated by lowering the administration concentration. In addition, successful construction of S-PPEGMA10-ICG also provides another choice for patients which experience allergy or other adverse effects when injected with existing contrast media, and therefore, the patients can gain diagnosis and treatment in time.

The invention relates to a dyeing method for digestive systems of acarid mites. The dyeing method is characterized by comprising the following steps: pouring 0.05 to 5 percent of sodium fluorescein or fuchsin solution into a petri dish under the condition that the temperature is 15 to 35DEG C and the humidity is 70 to 95 percent, and cutting a stretched parafilm into a circle of which the diameter is smaller than that of the petri dish; then laying the cut parafilm on the surface layer of the fluorescent sodium or fuchsin solution in the petri dish, and enabling the parafilm to float on the solution in the petri dish; finally, putting the acarid mites which have been starved for 48 to 72 hours on the parafilm, and observing the digestive systems of the acarid mites under a microscope after 3 hours. The dyeing method disclosed by the invention has the advantages that the operation is simple, and technical reference can be provided for observing digestive tracts and the whole digestive systems of the acarid mites under fluorescence or an ordinary stereomicroscope.