39 results about "Disodium Fluorescein" patented technology

Apparatus and method for measuring the fluorescence of nanodrop liquid samples is described in which the sample is held by surface tension between two anvil surfaces. Each anvil surface has an embedded optical fiber with its end finished flush with the surface in the containment area wetted by the sample with the fiber in line. Sample excitation is provided from the side of the sample remote from the containment area. By selection of the fiber transmission numeric aperture the impact of exciting and ambient light on the measurement is minimized. A method of virtual filtering is taught in which any ambient or exciting light that does impinge on the measuring sensor is corrected by subtracting a scaled representation of the source from the measurement. The method and apparatus is capable of detecting 1 femptomole of sodium fluorescein in 1 microliter of TE buffer.

Apparatus and method for measuring the fluorescence of nanodrop liquid samples is described in which the sample is held by surface tension between two anvil surfaces. Each anvil surface has an embedded optical fiber with its end finished flush with the surface in the containment area wetted by the sample with the fiber in line. Sample excitation is provided from the side of the sample remote from the containment area. By selection of the fiber transmission numeric aperture the impact of exciting and ambient light on the measurement is minimized. A method of virtual filtering is taught in which any ambient or exciting light that does impinge on the measuring sensor is corrected by subtracting a scaled representation of the source from the measurement. The method and apparatus is capable of detecting 1 femptomole of sodium fluorescein in 1 microliter of TE buffer.

The invention discloses an assay method of a migration path of groundwater by using a tracer agent. The assay method includes the following steps that a tracer area is determined and a ground map of the tracer area is drawn; the type of the tracer agent is determined; the delivery quantity of the tracer agent is determined; a delivery well and monitoring wells are selected and distributed; delivery and sampling of the tracer agent are carried out; detection of a tracer sample is carried out; a result of the groundwater migration path is summarized; and result interpretation of the groundwatermigration path is carried out. According to the assay method of the migration path of the groundwater by using the tracer agent, an assay method of a sodium fluorescein tracer agent is adopted, a time-concentration relation curve is drawn, according to the time-concentration relation curve and the straight line distance between the delivery well and the monitoring wells, the groundwater average velocity and the groundwater flow direction of the delivery well and the monitoring wells are measured, then hydraulic power relation conditions of the groundwater in and out of the tracer area are found out, the groundwater flow rate is determined, and the main direction of the groundwater flow direction is determined.

The invention belongs to the technical field of film preparation, and provides a light conversion plastic agricultural film with long service life and a preparation method thereof. The method comprises the following steps: stirring and dissolving an alcohol solution, stearic acid and liquid glass, and adding sodium fluorescein; performing dispersion, oscillation, suction filtration and vacuum drying to prepare stearic acid and liquid glass coated sodium fluorescein; mixing a film base material, thermoplastic starch, a light stabilizer, an antioxidant, an antifogging agent and a lubricating agent, extruding and performing blow molding to obtain the light conversion plastic agricultural film with long service life. Compared with a traditional method, the method has the advantages that a compact protective layer is formed by using stearic acid and liquid glass coated sodium fluorescein, oxygenolysis of sodium fluorescein can be effectively prevented, and the light conversion plastic agricultural film has relatively light-heat performance, good light conversion effect and long service life; and the preparation process is simple in operation and relatively low in cost, and has a wide application range.

The invention relates to a method for measuring the total antioxidant capacity of small berries, provides an ORAC detection kit suitable for the determination of the total antioxidant capacity of small berries and a detection method thereof, and belongs to the technical field of activity detection and determination. The kit includes: antioxidant standard substance Trolox storage reagent, fluorescent substance fluorescein sodium storage reagent, free radical generator AAPH reagent, phosphate buffer, and fluorescent microwell reaction plate, which is made of a plastic or hard paper material Boxes are packed together to make up. The ORAC detection method for the total antioxidant capacity of small berries comprises the following steps: small berry sample pretreatment; detection with reagent kits; and analysis of detection results. By mixing the sample and the reagent in a certain volume ratio, the fluorescence microplate analyzer is used to detect the change of the fluorescence intensity of the complex, thereby measuring and calculating the total antioxidant capacity of the small berry. The invention can provide a complete set of total antioxidant capacity test of small berries, has high sensitivity and good accuracy, and is convenient for popularization and application.

The invention discloses a full-automatic configuration and release control device of a rhodamine B fluorescent tracer and an operation method. The full-automatic configuration and release control device comprises a water supplying device, an integrated three-chamber combined box body, a central control device and an online real-time monitoring device, wherein the integrated three-room combined boxbody comprises a premixing chamber, a mixing chamber and a storage chamber which are communicated in sequence; the premixing chamber is used for storing dry powder of three tracers such as rhodamine,sodium fluorescein and tinopal; the mixing chamber comprises a temperature sensor, a stirring rod and a cooling base, and the temperature sensor is arranged at the lower part of the mixing chamber; the stirring rod is used for stirring solution to be mixed; the cooling base is positioned at the bottom part of the premixing chamber, the temperature sensor is connected with the central control device and the central control device controls the cooling base to realize temperature control in the mixing chamber; the storage chamber is used for storing prepared solution. The online real-time monitoring device is used for monitoring various parameters of the prepared solution, transmitting data to the central control device and retaining historical data in time.

Alkoxylated esters of dicarboxylic or tricarboxylic acids have an anti-irritating activity measured as a reduction in intracellular leakage of sodium fluorescein of 15% or more at 1:50 dilution or 40% or more at 1:100 dilution from a test composition containing 5% of the alkoxylated ester. These alkoxylated esters are useful in cosmetic and personal care products, especially in products such as baby shampoos, hand dishwashing liquids and bubble bath preparations. A method of reducing irritation provides for application of an irritant with the alkoxylated esters to the subject in a personal care or cosmetic product.

The invention discloses a preparation method for modified graphene. The preparation method comprises the following steps: preparing a graphene oxide / aqueous dispersion with a concentration of 0.01 to 3.0 mg / mL; adding sodium fluorescein into the graphene oxide / aqueous dispersion, and carrying out stirring; then adding an alkali solution and adjusting a pH value of a system to be 9 to 10; adding a chemical reducing agent, carrying out a reaction at 60 to 95 DEG C for 10 to 100 hours, and carrying out cooling to a room temperature; and subjecting the system to vacuum filtering or centrifuging, then carrying out washing with ethanol and deionized water to nearly neutral or neutral, and carrying out drying at 40 to 80 DEG C until a weight is constant so as to obtain the modified graphene. The preparation method provided by the invention is favorable for increasing and improving dispersibility of graphene in water and an organic solvent; meanwhile, the preparation method is simple, has mile condition, and is easy to operate and control.

The invention relates to a dyeing method of a tetranychid digestion system. The dyeing method is characterized in that under the conditions of the temperature being 20 to 35 DEG C and the humidity being 75 to 90 percent, 0.05 to 5 percent sodium fluorescein or fuchsine solution is poured into a culture dish; then, the stretched parafilm is sheared into a round shape with the diameter being smaller than that of the culture dish; then, the sheared parafilm is flatly put onto the surface layer of the sodium fluorescein or fuchsine solution contained in the culture dish so that the parafilm floats on the solution in the culture disc; finally, tetranychid after the hungry treatment for 24 to 48 hours is put onto the parafilm; after 2 to 4 hours, the tetranychid digestion system can be observed under fluorescence or microscope. The dyeing method has the advantages that the operation is simple; the practical technical reference can be provided for observing the alimentary tract and the whole digestion system of the tetranychid.

The invention discloses an engine coolant and a preparing method thereof. The engine coolant is prepared from, by weight, 500-700 parts of water, 40-60 parts of sodium formate, 5-15 parts of borax, 0.2-1.5 parts of potassium molybdate, 0.5-1.5 parts of octanedioic acid, 1-10 parts of sodium caprate, 0.5-1.5 parts of sodium nitrate, 0.5-1.5 parts of benzotriazole, 1-3 parts of sodium hydroxide, 1-3 parts of sodium metasilicate nonahydrate, 200-400 parts of 1,2-propylene glycol, 50-100 parts of glycerine, 0.001-0.008 part of sodium fluorescein, 0.01-0.08 part of simethicone, 0.05-0.15 part of lanthanum chloride, 0.05-0.15 part of chelating agent and 0.1-0.5 part of silicate stabilizer. The engine coolant is high in stability, freezing resistance, rust resistance and scaling resistance, capable of avoiding pipeline blocking, effectively preventing corrosion to ferrous metal, tin, copper and copper alloy, aluminum and aluminum alloy, overcoming the technical defect of traditional water-containing cooling liquid that boiling occurs easily, facilitating full-load and high-load operation of an engine and ensuring that a vehicle can travel safely in severe environments such as highlands, deserts and cold areas, and especially suitable for military vehicles, high-end vehicles and high-power heavy-load trucks and passenger cars.

The invention belongs to the technical field of antifreeze fluid and discloses an automotive antifreeze fluid and a preparation method thereof. The automotive antifreeze fluid is prepared from, by mass, 400.0-600.0%o of polyalcohol, 1.0-5.0%o of acrylic acid-acrylate-sulfonate ternary copolymer, 1.0-5.0%o of polyoxyethylene polyoxypropylene segmented copolymer, 5.0-10.0%o of organic amine, 0.5-5.0%o of benzotriazole, 0.5-5.0%o of accelerants, 0.5-5.0%o of sodium hydroxide, 0.5-5.0%o of sodium nitrate, 0.5-5.0%o of sodium metasilicate, 2.0-8.0%o of organic acid, 0.005-0.5%o of sodium fluorescein, 0.005-0.5%o of turquoise blue pigment, 0.005-0.5%o of an anti-foaming agent and the balance deionized water. The automotive antifreeze fluid has advantages of low freezing point, high boiling point, excellent anti-freezing and anti-boiling performances and great corrosion resistance and anti-scaling performances.

The invention discloses an application of combined IFA and FFA to merged DR and DI. The application method includes the following steps that 1, eye mydriasis is dropped for 30 minutes with tropicamide eye drops; 2, patients are subjected to a sodium fluorescein allergy test; 3, patients free of allergy phenomenon are subjected to IFA inspection; 4, then the patients are subjected to FFA inspection; 5, IFA inspection is repeated; 6, FFA inspection is repeated. Combined IFA and FFA inspection is compared with a past separated IFA inspection and FFA, merged-DR-and-DI patients can be comprehensively evaluated through combined-IFA-and-FFA inspection in one time of inspection, and the one-time-IFA-inspection cost is saved for all the patients. More importantly, early-merged-DR-and-DI patients can be discovered through combined-IFA-and-FFA inspection, the basis is provided for individualized treatment accordingly, and it is prevented that the illness state is evolved to blind neovascular glaucoma from NPDI or PDI.

The invention provides a method for testing the sealing integrity of a medicine packaging container by an improved color water intrusion method, and particularly relates to the technical field of sample pretreatment of medicines. The method comprises the steps of preparing a 1% sodium fluorescein solution, and putting the 1% sodium fluorescein solution into a sealing cavity of a sealing tester; inverting a prepared positive sample, submerging an inserted capillary tube by sterile powder, keeping the capillary tube immersed in a dye solution, and tightly covering the sealing cavity; and takingout the inverted positive sample after the invasion procedure is finished; adopting a sodium hydroxide solution with a concentration of 1 [mu] g / mL as a diluent; taking sterile powder for injection, which is not subjected to capillary insertion and color water invasion, fully redissolving the sterile powder by using the diluent, transferring the sterile powder into a clean centrifuge tube, and fixing the volume to 20mL; after the invasion procedure is finished, fully redissolving each bottle by using the diluent, transferring into a clean centrifuge tube, and fixing the volume to 20mL; and observing the dye invasion condition by naked eyes. According to the invention, the sensitivity of naked eye observation and detection can be significantly improved, the specificity is high, the anti-interference ability is strong, the detection efficiency is high, and the labor cost is saved.

The invention relates to a dyeing method of a digestive system of amblyseius tsugawai. The dyeing method is characterized by comprising the following steps of: in the conditions that the temperature is 25-30 DEG C and the humidity is 75-90%, pouring 0.05-5% of sodium fluorescein or an azaleine solution into a culture dish; then shearing a stretched parafilm to a circle, the diameter of which is smaller than the culture dish; then paving the sheared parafilm on the surface layer of the sodium fluorescein or the azaleine solution in the culture dish, wherein the parafilm floats on the solution of the culture dish; and finally, placing the amblyseius tsugawai starved for 0-72 hours on the parafilm to observe the digestive system of amblyseius tsugawai under a microscope within 1-3 hours. The dyeing method provided by the invention is simple to operate, and can provide technical reference to observe the digestive tract and the whole digestive system of the amblyseius tsugawai.

The invention relates to a staining method convenient for observing the digestive systems of predatory mites and prey flour mites. The staining method is characterized in that sodium fluorescein or Sudan red is mixed with a flour mite feed at the mass ratio of (1:1)-(1:10), the flour mites are fed with the feed mixed with sodium fluorescein or Sudan red, the flour mites of which the digestive systems can be conveniently observed can be obtained within 12-48 h, the obtained flour mites are continuously used for raising the predatory mites, and the predatory mites of which the digestive systems can be conveniently observed can be obtained within 12-48 h. The staining method is simple to operate, and can provide technical references for observing the digestive tracts and the whole digestive systems of the predatory mites and alternative preys thereof under a fluorescence microscope or a stereo-anatomical lens.

The invention discloses a preparation method of modified graphene. The graphene oxide / water dispersion with a concentration of 0.01-3.0 mg / mL is configured; sodium fluorescein is added to the graphene oxide / water dispersion and stirred; and then added Alkaline solution to adjust the pH of the system to 9-10; add a chemical reducing agent, react at 60-95 degrees Celsius for 10-100 hours, and cool to room temperature; filter or centrifuge the system, and wash with ethanol and deionized water until nearly neutral Or neutral, dry to constant weight at 40-80 degrees Celsius to obtain modified graphene. The invention is beneficial to increase and improve the dispersibility of graphene in water and organic solvents, and the preparation method is simple, the conditions are mild, and the operation and control are easy.

The invention relates to a staining method for digestive system of Neoseiulus barkeri, characterized in that 0.05-5% sodium fluorescein or fuchsine solution is poured into a culture dish under the temperature of 25-30 DEG C and the humidity of 75-90%, parafilm that is stretched is cut into a circle less than the culture dish in diameter, the cut parafilm is placed flat on the surface of the sodium fluorescein or fuchsine solution in the culture dish so that the parafilm floats on the solution in the culture dish, Neoseiulus barkeri that is hungry for 0-72 hours on the parafilm, and the digestive system of Neoseiulus barkeri may be observed under a microscope in 1-3 hours. The staining method is simple to perform, and allows the digestive tracts of Neoseiulus barkeri to be observed, providing technical reference for the whole digestive system.

The invention provides a bacillus subtilis synergist and application thereof for preparing bacillus subtilis fungicide, and belongs to the technical field of microbial preparation. The bacillus subtilis synergist is prepared from, by mass, 0.5-1.5 parts of xanthan gum, 1-3 parts of sodium fluorescein, 1-3 parts of a fluorescent brightener CBS-X, 1-3 parts of butylated hydroxytoluene, 6-9 parts ofsaccharose and 2-3 parts of potassium nitrate, wherein the xanthan gum, the sodium fluorescein and the fluorescent brightener CBS-X can achieve the good anti-ultraviolet protection effect on bacillussubtilis; the butylated hydroxytoluene can achieve the good anti-oxidation effect on the bacillus subtilis; the saccharose and the potassium nitrate can achieve the obvious synergism on the bacteriostatic activity of the bacillus subtilis. The ingredients can have the good biologic compatibility with the bacillus subtilis.

The invention relates to an experimental method for the relationship between the carbonization speed of concrete and the pore water saturation. In the sodium fluorescein solution, dye the unsaturated part of the pore water in the concrete specimen; cut the dyed concrete specimen in half along the diameter direction; one half of the concrete specimen is dyed with phenolphthalein to measure the carbonization depth, and the other half is placed Measure the saturated pore water depth under ultraviolet light irradiation, and calculate the carbonization speed according to the carbonization depth and carbonization time; replace the concrete specimen, change the carbonization time, and repeat the test to obtain multiple sets of pore water depth data and concrete carbonation corresponding to the pore water depth data. From the speed data, the relationship curve between concrete carbonation speed and pore water saturation is obtained. The invention makes up the blank of the effect of pore water saturation on the carbonization speed of concrete.

The invention relates to a cornea stainer. The cornea stainer comprises a carrier and a packaging bag. Cotton yarn or a cotton thread is adopted as the carrier, dye A is adsorbed on the carrier, and the dye A is sodium fluorescein. The carrier is placed in the packaging bag. Preferably, dye B is further adsorbed on the carrier, and the dye B is methyl blue or placenta blue or lissamine green. Morepreferably, the mass ratio of the dye A to the dye B is 1:1. According to the cornea stainer, the cotton yarn or the cotton thread replaces filter paper, and the defects that the dyeing effect of thefilter paper is poor, and the dyeing dosage carried by the carrier is small, specially the defect that when two or more dyeing agents are used for mixed dyeing, the dyeing effect of the filter paperis poor.

A composition and method for applying the composition topically to an eye for improving corneal and surface hydration and reducing blue light neuro-stimulation, especially during the several hours preceding sleep. In one embodiment, the composition combines an artificial tear with fluorescein sodium. The artificial tear may include carboxymehylcellulose sodium, polyvinyl alcohol, hydroxypropyl cellulose and glycerin. In another embodiment, the composition comprises synthetic human melanin combined with fluorescein sodium in an aqueous solution to reduce ocular UV light and HEV light exposure.

The invention relates to a method for observing digestive systems of amblyseius pswdolongis pinosus and prey tetranychus truncates thereof. The method is characterized by comprising the steps that 1-5 fresh kidney bean branches with two or more leaves are taken to be inserted into a conical flask containing a 0.05-0.5% sodium fluorescein water solution, tetranychus truncates hungered for 0-48 h is connected to the leaves after 24 h, tetranychus truncates with the digestive system convenient to observe can be obtained after 12-48 h, then, some tetranychus truncates continue to be adopted for feed amblyseius pswdolongis pinosus hungered for 0-48 h, and the amblyseius pswdolongis pinosus with the digestive system convenient to observe can be obtained after 12-48 h. The method is easy to implement, and references can be provided for observing digestive tracts and the whole digestive systems of the amblyseius pswdolongis pinosus and prey tetranychus truncates thereof under a common fluorescent microscope.

The invention provides a preparation process of cefuroxime sodium for injection. The cefuroxime sodium is dissolved in a phosphate buffer solution, activated carbon is used for decolorization, and the filter cake is washed with water to remove impurities in the cefuroxime sodium and prevent cefuroxime During the decolorization process, the sodium fluorescein degrades, and then the crystal seed, resin and acetone are added to the cefuroxime sodium solution for crystal growth, and the water absorption property of the resin is used to absorb the water in the solution during the crystallization process, increasing the proportion of acetone, and then Improve the crystallization effect of cefuroxime sodium, reduce the residual water in the crystal form, improve the stability of cefuroxime sodium for injection, and carry out pressure treatment at regular intervals, which can change the water absorption and absorption of the resin when the crystallization reaches the bottleneck. The solubility of cefuroxime sodium promotes the precipitation of cefuroxime sodium crystals.

The invention relates to a PEG nuclear-crosslinked star high-molecular nano contrast agent which is a star polymer having a PEG core prepared by bonding fluorescent groups. The invention also relatesto application of the PEG nuclear-crosslinked star high-molecular nano contrast agent in the preparation of fundal vascular contrast media. Part of patients intravenously injected with fundal vascularcontrast media clinically applied at present, such as sodium fluorescein or indocyanine green, experience adverse effects; these patients cannot take complete contrast examination. Compared with theprior contrast media, S-PPEGMA10-ICG herein has the advantages of low injection concentration and better choroidal development clarity; the side effects of prior drugs can be alleviated by lowering the administration concentration. In addition, successful construction of S-PPEGMA10-ICG also provides another choice for patients which experience allergy or other adverse effects when injected with existing contrast media, and therefore, the patients can gain diagnosis and treatment in time.

The invention relates to a dyeing method for digestive systems of acarid mites. The dyeing method is characterized by comprising the following steps: pouring 0.05 to 5 percent of sodium fluorescein or fuchsin solution into a petri dish under the condition that the temperature is 15 to 35DEG C and the humidity is 70 to 95 percent, and cutting a stretched parafilm into a circle of which the diameter is smaller than that of the petri dish; then laying the cut parafilm on the surface layer of the fluorescent sodium or fuchsin solution in the petri dish, and enabling the parafilm to float on the solution in the petri dish; finally, putting the acarid mites which have been starved for 48 to 72 hours on the parafilm, and observing the digestive systems of the acarid mites under a microscope after 3 hours. The dyeing method disclosed by the invention has the advantages that the operation is simple, and technical reference can be provided for observing digestive tracts and the whole digestive systems of the acarid mites under fluorescence or an ordinary stereomicroscope.

The invention discloses an engine coolant and a preparing method thereof. The engine coolant is prepared from, by weight, 500-700 parts of water, 40-60 parts of sodium formate, 5-15 parts of borax, 0.2-1.5 parts of potassium molybdate, 0.5-1.5 parts of octanedioic acid, 1-10 parts of sodium caprate, 0.5-1.5 parts of sodium nitrate, 0.5-1.5 parts of benzotriazole, 1-3 parts of sodium hydroxide, 1-3 parts of sodium metasilicate nonahydrate, 200-400 parts of 1,2-propylene glycol, 50-100 parts of glycerine, 0.001-0.008 part of sodium fluorescein, 0.01-0.08 part of simethicone, 0.05-0.15 part of lanthanum chloride, 0.05-0.15 part of chelating agent and 0.1-0.5 part of silicate stabilizer. The engine coolant is high in stability, freezing resistance, rust resistance and scaling resistance, capable of avoiding pipeline blocking, effectively preventing corrosion to ferrous metal, tin, copper and copper alloy, aluminum and aluminum alloy, overcoming the technical defect of traditional water-containing cooling liquid that boiling occurs easily, facilitating full-load and high-load operation of an engine and ensuring that a vehicle can travel safely in severe environments such as highlands, deserts and cold areas, and especially suitable for military vehicles, high-end vehicles and high-power heavy-load trucks and passenger cars.

The invention provides a bacillus subtilis synergist and application thereof for preparing bacillus subtilis fungicide, and belongs to the technical field of microbial preparation. The bacillus subtilis synergist is prepared from, by mass, 0.5-1.5 parts of xanthan gum, 1-3 parts of sodium fluorescein, 1-3 parts of a fluorescent brightener CBS-X, 1-3 parts of butylated hydroxytoluene, 6-9 parts ofsaccharose and 2-3 parts of potassium nitrate, wherein the xanthan gum, the sodium fluorescein and the fluorescent brightener CBS-X can achieve the good anti-ultraviolet protection effect on bacillussubtilis; the butylated hydroxytoluene can achieve the good anti-oxidation effect on the bacillus subtilis; the saccharose and the potassium nitrate can achieve the obvious synergism on the bacteriostatic activity of the bacillus subtilis. The ingredients can have the good biologic compatibility with the bacillus subtilis.

The invention belongs to the technical field of ophthalmological clinical detection supplies, and particularly relates to a test strip for eye detection. The test strip comprises a test strip body; afold is arranged at a place which is 5mm away from the top of the test strip body; the test strip body above the fold is elliptical; a scale is arranged below the fold on the front of the test strip body; a sodium fluorescein solution layer is arranged in a 2-3mm scale area below the fold; a word left or right is written below the scale to distinguish the use of left and right eyes; a sodium fluorescein solution layer is arranged at the middle part of the front of the test strip body above the fold; and a special mark layer is arranged in a 10-25mm area of the scale at the front of the test strip body. The test strip is capable of solving the problems that the square ends of the existing Schirme test strips are easy to stimulate the ocular tissues of the patients during the detection so asto cause eye discomfort to the patients and the subsequent tear film breakup time measurement and cornea staining detection need to drip sodium fluorescein solution in conjunctival sac, and has the advantages of being convenient and rapid to use, simple to operate and convenient to store data.

The invention relates to a PEG core cross-linked star-shaped macromolecule nano-contrast agent. The contrast agent is a star-shaped macromolecule with PEG as the core and is prepared by bonding a fluorescent group. The invention also relates to the application of the PEG core cross-linked star-shaped macromolecule nano-contrast material in the preparation of fundus vascular contrast agent. Fundus angiography currently used clinically, such as sodium fluorescein or indocyanine green, has adverse reactions when intravenous contrast agents are injected in some patients, and these patients cannot complete the angiography examination. Compared with existing contrast agents, S-PPEGMA of the present invention 10 ‑ICG has the advantages of lower injection concentration and clearer choroid visualization, which can reduce the side effects of the original drug by reducing the administration concentration. In addition, S‑PPEGMA 10 ‑The successful construction of ICG also provides another option for patients who have allergies or other adverse reactions to the injection of existing contrast agents, so that patients can be diagnosed and treated in a timely manner.

The invention relates to a dyeing method for conveniently observing digestive systems of predatory mites and prey tetranychidae of the predatory mites. The dyeing method is characterized by including the steps that 1-5 pieces of fresh kidney bean branches with two or above leaves are taken and inserted into a conical flask containing a 0.05% to 0.5%sodium-fluorescein water solution, tetranychidae hungering for 0 hour to 48 hours are placed on the leaves after 24 hours for feeding the tetranychidae, the digestive-system-conveniently-observed tetranychidae can be obtained after 12 hours to 48 hours, the tetranychidae continues to be used for feeding the predatory mites hungering for 0 hour to 48 hours, and the digestive-system-conveniently-observed predatory mites can be obtained after 12 hours to 48 hours. The dyeing method is easy to operate, and references can be provided for the aim that digestive tracts and whole digestive systems of the predatory mites and predatory-mite prey tetranychidae can be observed under a common phosphor-type microscope.