A bacillus subtilis synergist and its application in the preparation of bacillus subtilis inoculum

A technology of Bacillus subtilis and synergist, which is applied in the application, fungicides, chemicals used for biological control, etc., can solve the problems such as poor efficacy of medicine, poor storage stability of preparations, gap in biological activity, etc. Achieve good UV protection, good biocompatibility, good antioxidant effect

Active Publication Date: 2020-11-06
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The important influencing factors are that microorganisms are sensitive to external environmental factors such as temperature, humidity and light, and the storage stability of the preparation is poor, and it is also easily affected by environmental factors after application, so that the drug effect cannot be well exerted.
In addition, in general, the biological activity of biological agents has a certain gap compared with chemical pesticides
[0005] Another difficulty in processing microorganisms into preparations is that their compatibility with various adjuvants is generally worse than that of chemical pesticides, and some adjuvants may not be used at all

Method used

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  • A bacillus subtilis synergist and its application in the preparation of bacillus subtilis inoculum
  • A bacillus subtilis synergist and its application in the preparation of bacillus subtilis inoculum
  • A bacillus subtilis synergist and its application in the preparation of bacillus subtilis inoculum

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preparation example Construction

[0030] In the present invention, the preparation method of the former powder of Bacillus subtilis preferably comprises the following steps:

[0031] (1) Inoculate the test tube species of Bacillus subtilis in a broth medium shake flask, shake and cultivate to the logarithmic phase, and obtain the activated bacterial species;

[0032] (2) The activated strain is inserted into the seed tank according to the volume ratio of 8-12% of the seed tank culture medium, and the seed tank is cultivated to the logarithmic growth phase to obtain the seed liquid;

[0033] (3) The seed liquid is inserted into the production tank according to the volume ratio of 8 to 12% of the production tank culture medium, and the production tank is cultivated until the final biomass ≥ 10 10 CFU / g, spore formation rate ≥ 97%, the fermentation is completed, and the culture solution is obtained;

[0034] (4) Spray drying after filtering the culture solution to obtain the former powder of Bacillus subtilis

...

Embodiment 1

[0042] Weigh 1g xanthan gum, 2g sodium fluorescein, 2g fluorescent whitening agent CBS-X, 2g dibutylhydroxytoluene, 7.5g sucrose and 2.5g potassium nitrate. Then mix the above-mentioned raw materials obtained by weighing evenly, control the processing temperature at about 25° C., pulverize in a jet mill, and pass through a 150-mesh sieve to obtain the Bacillus subtilis synergist.

Embodiment 2

[0044] Weigh 1g xanthan gum, 2g sodium fluorescein, 2g fluorescent whitening agent CBS-X, 2g dibutylhydroxytoluene, 7.5g sucrose, 2.5g potassium nitrate, 3g sodium lauryl sulfate, 2g methylenebis Sodium naphthalenesulfonate and 2g polyoxyethylene glyceryl ether. Then mix the above-mentioned raw materials obtained by weighing evenly, control the processing temperature at about 25° C., pulverize in a jet mill, and pass through a 150-mesh sieve to obtain the Bacillus subtilis synergist.

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Abstract

The invention provides a bacillus subtilis synergist and application thereof for preparing bacillus subtilis fungicide, and belongs to the technical field of microbial preparation. The bacillus subtilis synergist is prepared from, by mass, 0.5-1.5 parts of xanthan gum, 1-3 parts of sodium fluorescein, 1-3 parts of a fluorescent brightener CBS-X, 1-3 parts of butylated hydroxytoluene, 6-9 parts ofsaccharose and 2-3 parts of potassium nitrate, wherein the xanthan gum, the sodium fluorescein and the fluorescent brightener CBS-X can achieve the good anti-ultraviolet protection effect on bacillussubtilis; the butylated hydroxytoluene can achieve the good anti-oxidation effect on the bacillus subtilis; the saccharose and the potassium nitrate can achieve the obvious synergism on the bacteriostatic activity of the bacillus subtilis. The ingredients can have the good biologic compatibility with the bacillus subtilis.

Description

technical field [0001] The invention belongs to the technical field of microbial preparations, and in particular relates to a bacillus subtilis synergist and its application in preparing a bacillus subtilis bacterial agent. Background technique [0002] Tobacco black shank and tobacco red spot are important diseases in tobacco production. Disease control relies heavily on chemical control, but chemical control has many disadvantages. The first is to cause environmental pollution and pesticide residues; the second is to produce drug resistance, the control effect is reduced, and the cost of control is increased; the third is that the pesticide is greatly affected by the environment, and the control effect is not ideal. Compared with chemical control, biological control is not only environmentally friendly, but also has the characteristics of better effect, which has always been one of the research hotspots. [0003] Bacillus subtilis Tpb55 has a good control effect on variou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N25/22A01N63/22A01P3/00C12R1/125
CPCA01N25/22A01N63/00C12N1/20
Inventor 张成省赵栋霖裴晖孔凡玉王凤龙陈德鑫高加明李锡宏王瑞郭利王静李义强
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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