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Method for observing digestive systems of amblyseius pswdolongis pinosus and prey tetranychus truncates thereof

A technology for the digestive system of A. longiseius and its digestive system, which can be used in animal husbandry and other fields. It can solve the problems that there is no method for observing the digestive system of A. longiseius and its prey, Tetranychus truncation, and achieve the effect of simple operation.

Inactive Publication Date: 2017-06-20
合肥天敌之家生物技术有限公司
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Problems solved by technology

[0004] However, so far, there is no relevant method in China to facilitate the observation of the digestive system of Amblyseius pseudophyllosa and its prey, Tetranychus truncates

Method used

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Examples

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Embodiment

[0008] Example The dyeing method of Tetranychus truncation and Amblyseius pseudoviresus

[0009] Pick several spider mites into a 2ml centrifuge tube and starve for 24 hours for later use. Then insert 3-4 branches of fresh kidney bean branches with 3-4 leaves in each conical flask filled with 500 ml of 0.1% sodium fluorescein aqueous solution. After 24 hours, the leaves of kidney beans can be completely dyed. The leaves were connected with Tetranychus truncates, and after 24 hours, the fluorescence of the whole body of Tetranychus truncates could be observed under a higher magnification of a fluorescent stereomicroscope. After the Tetranychus truncatus was stained with fluorescent light, the leaves were fed with A. truncatus which had been starved for 24 hours, or the dyed Tetranychus truncatus was picked from the leaves and starved for A. truncatus in the breeding chamber. 24 hours later, under the higher magnification of the fluorescent stereomicroscope, it can be observed ...

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Abstract

The invention relates to a method for observing digestive systems of amblyseius pswdolongis pinosus and prey tetranychus truncates thereof. The method is characterized by comprising the steps that 1-5 fresh kidney bean branches with two or more leaves are taken to be inserted into a conical flask containing a 0.05-0.5% sodium fluorescein water solution, tetranychus truncates hungered for 0-48 h is connected to the leaves after 24 h, tetranychus truncates with the digestive system convenient to observe can be obtained after 12-48 h, then, some tetranychus truncates continue to be adopted for feed amblyseius pswdolongis pinosus hungered for 0-48 h, and the amblyseius pswdolongis pinosus with the digestive system convenient to observe can be obtained after 12-48 h. The method is easy to implement, and references can be provided for observing digestive tracts and the whole digestive systems of the amblyseius pswdolongis pinosus and prey tetranychus truncates thereof under a common fluorescent microscope.

Description

technical field [0001] The invention relates to a dyeing method for conveniently observing the digestive system of Amblyseius pseudophyllosa and its prey, Tetranychus truncates, and belongs to the field of biological control of modern agricultural pests. Background technique [0002] At present, domestic research on spider mites is mainly limited to classification, breeding and application, and there is little research on the microstructure of mites, especially the mechanism of the digestive system. [0003] The digestive system of Tetranychus truncation is an important structure for studying the food it eats and digests. It is possible to observe the direction of the food in the mite body after the mite eats the food. Significance. [0004] However, so far, there is no relevant method in China on how to observe the digestive system of Amblyseius plastiformis and its prey, Tetranychus truncation. Contents of the invention [0005] The purpose of the present invention is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 王伯明杨静逸邵显芝
Owner 合肥天敌之家生物技术有限公司
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