Staining method for digestive system of Neoseiulus barkeri

A technology of Neoseiid mite and digestive system, which is applied in the field of modern agricultural pest biological control to achieve the effect of simple operation

Inactive Publication Date: 2017-07-21
合肥天敌之家生物技术有限公司
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  • Summary
  • Abstract
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Problems solved by technology

[0004] However, there is no report on how to easily observe the digestive system of Neoseiius pasteurii

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0009] Example The staining method of Neoseius pasterii

[0010] At a temperature of 24°C and a humidity of 85%, pour 30ml of 0.1% sodium fluorescein into a petri dish with a diameter of 5cm, then stretch the parafilm film, cut out a circle with a diameter of 3cm, and then place the cut parafilm Place the membrane flat on the surface of the sodium fluorescein solution in the petri dish so that the parafilm membrane floats on the petri dish solution, and finally place the Neoseius pasteruii that has been starved for 60 hours in a 2 ml centrifuge tube on the film, 1 The digestive system of Neoseiusius pasteruii can be observed under a fluorescent stereomicroscope within hours.

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PUM

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Abstract

The invention relates to a staining method for digestive system of Neoseiulus barkeri, characterized in that 0.05-5% sodium fluorescein or fuchsine solution is poured into a culture dish under the temperature of 25-30 DEG C and the humidity of 75-90%, parafilm that is stretched is cut into a circle less than the culture dish in diameter, the cut parafilm is placed flat on the surface of the sodium fluorescein or fuchsine solution in the culture dish so that the parafilm floats on the solution in the culture dish, Neoseiulus barkeri that is hungry for 0-72 hours on the parafilm, and the digestive system of Neoseiulus barkeri may be observed under a microscope in 1-3 hours. The staining method is simple to perform, and allows the digestive tracts of Neoseiulus barkeri to be observed, providing technical reference for the whole digestive system.

Description

technical field [0001] The invention relates to a dyeing method for the digestive system of Neoseius pasterii, belonging to the field of biological control of modern agricultural pests. Background technique [0002] At present, domestic research on Neoseiius pasterei is mainly limited to the classification, breeding and application, and there is little research on the microstructure of Neoseiius pasteruii, especially the mechanism of the digestive system. [0003] The study of the digestive system of Neoseius pastereii is an important structure for studying the food it eats and digests. It is possible to observe the direction of the food in the body of the mite after Neoseius pasterei eats food. System, as well as easy and effective marking of the direction of food is of great significance. [0004] However, there is no report on how to easily observe the digestive system of Neoseius pasterei. [0005] The purpose of the present invention is to provide a simple and efficie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
CPCG01N1/30
Inventor 王伯明杨静逸邵显芝
Owner 合肥天敌之家生物技术有限公司
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