Dyeing method of digestive system of amblyseius tsugawai

A technology of digestive system and dyeing method, applied in the preparation of test samples, etc., to achieve the effect of simple operation

Inactive Publication Date: 2017-07-18
山东首伯农生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, there is no report on how to easily

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0009] Example Staining method of Amblyseius tsukawa

[0010] At a temperature of 24°C and a humidity of 85%, pour 30ml of 0.1% sodium fluorescein into a petri dish with a diameter of 5cm, then stretch the parafilm film, cut out a circle with a diameter of 3cm, and then place the cut parafilm Place the membrane on the surface of the sodium fluorescein solution in the petri dish so that the parafilm membrane floats on the petri dish solution, and finally put the A. tsugawan starved for 60 hours in a 2 ml centrifuge tube on the film, and the parafilm will be ready for 1 hour. The digestive system of A. tsugawan can be observed under a fluorescent stereomicroscope.

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Abstract

The invention relates to a dyeing method of a digestive system of amblyseius tsugawai. The dyeing method is characterized by comprising the following steps of: in the conditions that the temperature is 25-30 DEG C and the humidity is 75-90%, pouring 0.05-5% of sodium fluorescein or an azaleine solution into a culture dish; then shearing a stretched parafilm to a circle, the diameter of which is smaller than the culture dish; then paving the sheared parafilm on the surface layer of the sodium fluorescein or the azaleine solution in the culture dish, wherein the parafilm floats on the solution of the culture dish; and finally, placing the amblyseius tsugawai starved for 0-72 hours on the parafilm to observe the digestive system of amblyseius tsugawai under a microscope within 1-3 hours. The dyeing method provided by the invention is simple to operate, and can provide technical reference to observe the digestive tract and the whole digestive system of the amblyseius tsugawai.

Description

technical field [0001] The invention relates to a dyeing method for the digestive system of the amblyseius tsukawa, belonging to the field of biological control of modern agricultural pests. Background technique [0002] At present, domestic research on A. jinsichuan is mainly limited to the classification, breeding and application. There is little research on the microstructure of A. jinchuan, especially the mechanism of the digestive system. [0003] The study of the digestive system of A. Jinchuan mite is an important structure for studying the food it eats and digests. It is possible to observe the direction of the food in the mite body after A. Jinchuan mite eats food. It is a simple and effective marker for the study of the digestive system of A. Where the food goes is significant. [0004] However, there is no report on how to easily observe the digestive system of A. tsugawan. [0005] The purpose of the present invention is to provide a simple and efficient dyeing...

Claims

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Application Information

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IPC IPC(8): G01N1/30
CPCG01N1/30
Inventor 姜晓环毕思佳何永娟
Owner 山东首伯农生物技术有限公司
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