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Dyeing method for digestive systems of acarid mites

A technology of digestive system and dyeing method, which is applied in a dyeing field of the digestive system of acaroid mites, to achieve the effect of simple operation

Inactive Publication Date: 2017-08-18
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is no report on how to easily observe the digestive system of acaroid mites

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0009] Example Dyeing method of acaroid mites

[0010] At a temperature of 25°C and a humidity of 85%, pour 30ml of 0.1% sodium fluorescein into a petri dish with a diameter of 5cm, then stretch the parafilm film, cut out a circle with a diameter of 3cm, and then place the cut parafilm film Place the surface layer of the sodium fluorescein solution in the petri dish so that the parafilm membrane floats on the petri dish solution, and finally place the acarid mites that have been starved for 72 hours in a 2 ml centrifuge tube on the film, and they can be released after 5 hours The digestive system of the acarid mite was observed under a fluorescent stereomicroscope.

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PUM

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Abstract

The invention relates to a dyeing method for digestive systems of acarid mites. The dyeing method is characterized by comprising the following steps: pouring 0.05 to 5 percent of sodium fluorescein or fuchsin solution into a petri dish under the condition that the temperature is 15 to 35DEG C and the humidity is 70 to 95 percent, and cutting a stretched parafilm into a circle of which the diameter is smaller than that of the petri dish; then laying the cut parafilm on the surface layer of the fluorescent sodium or fuchsin solution in the petri dish, and enabling the parafilm to float on the solution in the petri dish; finally, putting the acarid mites which have been starved for 48 to 72 hours on the parafilm, and observing the digestive systems of the acarid mites under a microscope after 3 hours. The dyeing method disclosed by the invention has the advantages that the operation is simple, and technical reference can be provided for observing digestive tracts and the whole digestive systems of the acarid mites under fluorescence or an ordinary stereomicroscope.

Description

technical field [0001] The invention relates to a dyeing method for the digestive system of acarina mites, belonging to the field of biological control of modern agricultural pests. Background technique [0002] At present, domestic research on acaroid mites is mainly limited to classification, breeding and application, and there is little research on the microstructure of acaroid mites, especially the mechanism of the digestive system. [0003] The digestive system of acaroid mites is an important structure for the study of food mites feeding and digesting food. It is of great significance to study the digestive system of acaroid mites and to easily and effectively mark the direction of food. . [0004] However, there is no report on how to easily observe the digestive system of acaroid mites. [0005] The purpose of the present invention is to provide a simple and efficient dyeing method for the digestive system of acaroid mites without causing death of mites. Contents...

Claims

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Application Information

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IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 吕佳乐刘珊贾冰红徐学农岳瑾董杰乔岩周长青王品舒宗静王伟青王恩东
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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