CRISPR detection primer group for mycoplasma pneumoniae and application of CRISPR detection primer group

A technology for detection of mycoplasma pneumoniae and primers, applied in the direction of microbe-based methods, microbiological measurement/testing, microbiology, etc., can solve the problem of inability to improve sensitivity, and achieve the goal of getting rid of the dependence on complex temperature-variable amplification instruments, high sensitivity, and broad The effect of applying the foreground
CN110804669AActive Publication Date: 2020-02-18广州微远医疗器械有限公司 +3
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
广州微远医疗器械有限公司
Publication Date
2020-02-18

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Abstract

The invention relates to a CRISPR (clustered regularly interspaced short palindromic repeat) detection primer group for mycoplasma pneumoniae and an application of the CRISPR detection primer group, and belongs to the technical field of gene detection of a CRISPR technology. The primer group comprises an amplification primer pair and crRNA (CRISPR-derived ribonucleic acid), wherein the amplification primer pair is used for amplifying a sequence shown as SEQ ID NO. (sequence identifier number) 1 of the mycoplasma pneumoniae; crRNA comprises an anchor sequence and a guide sequence; the anchor sequence specifically identifies a Cas protein; and the guide sequence is matched with a target sequence segment in the sequence of SEQ ID NO. 1. The primer group detects the mycoplasma pneumoniae through the CRISPR technology; the detection time of the mycoplasma pneumoniae is shortened; and the detection can be accomplished within 60min. A specific sequence combination obtained by screening acts as the primer group for detection, the primer group has the advantages of high sensitivity and strong specificity, and a limit of detection of the primer group can reach 30 copies. The primer group isused for the CRISPR detection of the mycoplasma pneumoniae, is independent of a complicated variable temperature amplification instrument such as a qPCR (quantitative polymerase chain reaction) instrument, and a CRISPR-Cas technology has wide application prospects in instant diagnosis of the mycoplasma pneumoniae.
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Description

technical field

[0001] The invention relates to the technical field of gene detection based on CRISPR technology, in particular to a CRISPR detection primer set for Mycoplasma pneumoniae and its application. Background technique

[0002] Mycoplasma pneumoniae infection (Mycoplasma pneumonia infection) exists widely in all parts of the world. The infection is transmitted from person to person through the droplets sprayed by patients when they cough. About 80% of patients will have obvious clinical manifestations, usually manifested as pharyngitis, tracheobronchitis, reaction Acute airway disease / wheeze or nonspecific upper respiratory syndrome. The incubation period of Mycoplasma pneumoniae is 2 to 4 weeks, so infection in certain populations can last for several weeks. Mycoplasma pneumoniae disease usually occurs in densely populated places such as military bases, boarding schools, and summer camps. The family infection rate of children is 84%, while that of adults is 41%. ...

Claims

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