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Rice lesion mimic gene and encoded protein thereof

A protein and lesion-like technology, which is applied in genetic engineering, plant genetic improvement, and the use of vectors to introduce foreign genetic material, etc., can solve the problem of low disease resistance and achieve good results, easy application, and simple identification and selection.

Inactive Publication Date: 2016-12-21
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disease resistance of rice is directly related to the blood background of the parental materials. Most of the cloned lesion-like genes are from the background of japonica rice, but there are few reports in indica rice, which also limits the use of lesion-like materials in improving the disease resistance of indica rice. Applications

Method used

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  • Rice lesion mimic gene and encoded protein thereof
  • Rice lesion mimic gene and encoded protein thereof
  • Rice lesion mimic gene and encoded protein thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Isolation and genetic analysis test of the lesion-like mutant a344 ​​of the present invention

[0039] (1) Test materials

[0040] Lesion-like mutants a344, Hua B and Shuhui 498 were all from the Laboratory of Heterosis Utilization, Rice Research Institute, Sichuan Agricultural University. Among them, the lesion-like mutant a344 ​​was derived from the EMS (ethyl methylsulfonate) mutagenesis library constructed by Shuhui 498. It was found after multiple generations of backcrossing with Shuhui 498 in Wenjiang, Sichuan and Lingshui, Hainan. The lesion phenotype can be stably inherited.

[0041] (2) Test method

[0042] Lesion-like mutants a344, Hua B and Shuhui 498 were all planted in Wenjiang Experimental Field of Rice Research Institute of Sichuan Agricultural University. F 2 Groups, Statistics F 1 and F 2 group separation ratio.

[0043] (1) Lesion-like phenotype analysis: Compared with wild-type Shuhui 498, mutant a344 ​​appeared necrotic spots on the ...

Embodiment 2

[0048] Example 2 Comparison test of the present invention's lesion-like mutant a344 ​​for identification of resistance to bacterial blight

[0049] (1) Test materials

[0050] 1. Rice material

[0051] (1), Mutants a344 ​​and Shuhui 498 were all derived from the Laboratory of Heterosis Utilization, Rice Research Institute, Sichuan Agricultural University.

[0052] (2) IR24, a variety highly susceptible to bacterial blight, and IRBB21, a variety highly resistant to bacterial blight, were both from the Major Disease Research Office of the Rice Research Institute of Sichuan Agricultural University.

[0053] 2. Physiological races of Xanthomonas oryzae: J9, 8248, P2, P6, J3 or J7, all from the Major Disease Research Office of the Rice Research Institute of Sichuan Agricultural University.

[0054] (2) Test method

[0055] 1. Cultivation of rice bacterial blight

[0056] Take the bacterial blight of rice preserved in glycerol, smear evenly on the solid medium, and incubate in the...

Embodiment 3

[0064] Embodiment 3: the localization test of mutant a344 ​​candidate gene

[0065] Proceed as follows:

[0066] 1. Location of candidate genes

[0067] (1) Construction of near isogenic pool

[0068] F obtained from crossing mutant a344 ​​with flower B 2 In the population, randomly select 30 leaves of a single plant with a lesion-like phenotype and 10 leaves of a normal single plant, and mix and extract DNA from every 10 leaves in equal amounts to build a pool, including 3 recessive pools and 1 dominant pool A total of 4 pools were used for polymorphism detection between mutant a344 ​​and flower B genome. F obtained by crossing a344 ​​with Shuhui 498 2 In the population, 70 leaves of individual plants with lesion-like phenotype were randomly selected, and equal amounts of leaves from every 10 plants were mixed to extract DNA to build a pool. A total of 7 pools were used for co-segregation analysis of mutation sites. Leaf DNA was extracted by the improved CTAB method.

...

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Abstract

The invention discloses a protein for controlling rice lesion mimic characters. The protein is formed by an amino acid sequence as shown by SEQ ID NO. 2 in a sequence table or a derivative sequence thereof. The invention further discloses a gene for encoding the protein. The gene is formed by a nucleotide sequence as show by SEQ ID NO. 1 in the sequence table. The lesion mimic gene provided by the invention has a good effect on resistance to rice bacterial blight, can be used for improving the bacterial blight resistance of nonglutinous rice, and provides a new way for breeding the nonglutinous rice capable of resisting bacterial blight; secondly, a new gene knockout technology is applied, a lesion mimic material under the background of japonica rice is acquired successfully, and a new method for utilizing the gene to culture japonica rice variety capable of resisting bacterial blight is provided. In addition, the gene provided by the invention is controlled by a single recessive gene, a transgene or filial generation can be simply identified and selected, and the convenience in application is realized.

Description

technical field [0001] The invention belongs to the field of rice genetic engineering, and in particular relates to a rice lesion-like gene and a protein encoded by the gene. Background technique [0002] Plant lesions refer to the phenomenon that the plants spontaneously form necrotic spots similar to those caused by a certain pathogen infection without any obvious adversity stress, physical damage or pathogen invasion. Many lesion-like mutants exhibit enhanced resistance to pathogens either before or after lesion-like formation. The rice lesion-like mutants that can be stably inherited all conform to the classic Mendelian inheritance law. Nearly 200 lesion-like mutants have been reported in rice, and most of their lesion-like traits are controlled by recessive mononuclear genes. At present, 10 genes controlling lesion-like traits have been cloned, mainly SPL5 encoding splicing factor 3b subunit, SPL11 encoding E3 ubiquitin ligase, etc. (Sun Huimin et al. Shanghai Agricult...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00
CPCC07K14/415C12N15/8281
Inventor 钦鹏李仕贵邓路长马炳田王玉平钟光荣
Owner SICHUAN AGRI UNIV
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