Tissue culture method of huperizia serrata

Abstract

The invention discloses a method for tissue culture method of huperizia serrata. The method comprises the following steps of: selecting an explant; killing surface bacteria; performing inoculation and cultivation; killing endophyte; performing propagation cultivation; and performing rooting cultivation. The huperizia serrata is a precious medicinal pteridophyte which has a tough requirement on a habitat, grows slowly under a natural condition and has a long production period. In a conventional method, propagation coefficient and biomass are low and requirements on medicament production cannot be met. Due to the adoption of the method, the differentiation rate of a huperizia serrata test tube plantlet is up to 90 percent, monthly multiplication multiple is up to 2.1 to 3.5, rooting rate is up to 100 percent, a root system grows quickly, root number is up to 1.6 to 3.4 per plant, a regeneration seedling grows healthily and the root system is developed. The method has the advantages of effectively restraining the pollution and browning of a huperizia serrata explant, promoting the survival and growth of the explant and providing important technical support for the realization of manual mass planting of the huperizia serrata along with simpleness, convenience, practicability, high efficiency and low cost.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for establishing a Huperzia serrata tissue culture system. Background technique [0002] Huperzia serrata (Thunb.) Trev., also known as the King of Help, Jinbuchang, Melaleuca, Sanzhi, Serpentine, belongs to the fern Huperiaceae (Huperiaceae) Huperzia Bemh., is a A valuable medicinal fern. The whole herb is commonly used among the people to treat bruises, congestion, swelling, poison, schizophrenia and other diseases. The Huperzine A (Huperzine A) contained in it has been proved by pharmacological experiments that it can inhibit acetylcholinesterase (AchE) with low toxicity, high efficiency, reversibility and high selectivity. Weakness, improving learning efficiency and improving the memory function of the elderly, etc., are currently specific drugs for the treatment of diseases such as senile dementia, and have a broad market prospect. Due to...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of difficult explant sterilization, low rate of acquisition of sterile explants, serious pollution and browning of explants after inoculation, and failure of normal growth, differentiation and proliferation in the tissue culture process of Huperz serrata, the present invention Through the research on the sterilization method, culture medium and culture conditions, the lower pollution rate, low browning rate, high survival rate, high growth rate, high differentiation rate and high rooting rate of Huperz serrata tissue culture have been realized; the present invention Aiming at providing a method for tissue culture of Huperz serrata