Endophyte and application thereof

A technology of plant endophytes and plants, applied in the direction of plant growth regulators, plant growth regulators, applications, etc., to achieve the effects of promoting absorption, improving soil physical and chemical properties, and efficient transformation

Active Publication Date: 2013-09-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been reports on the use of aerobic denitrification microorganisms to treat nitrate wastewater, but there are few reports on the use of microorganisms to effectively reduce the nitrate content in the soil and improve the secondary salinization of the soil

Method used

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  • Endophyte and application thereof
  • Endophyte and application thereof
  • Endophyte and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The separation of embodiment 1 Sedum sedum endophytes

[0033] (1) culture medium

[0034] DF medium: every liter of culture medium contains KH 2 PO 4 4g, Na 2 HPO 4 6g, MgSO 4 ·7H 2 O0.2g, glucose 2g, sodium gluconate 2g, citric acid 2g, (NH 4 ) 2 SO 4 2 g, 0.1 mL each of component 1 and component 2 solutions, 15 g of agar, the solvent is water, pH 7.2, and sterilized by high pressure steam (121°C, 20 min).

[0035] Among them, component 1: H 3 BO 3 10mg, MnSO 4 ·H 2 O11.19mg, ZnSO 4 ·7H 2 O124.6mg, CuSO 4 ·5H 2 O78.22mg, MoO 3 Dissolve 10mg in 100mL sterilized distilled water and store at -4°C;

[0036] Component 2: FeSO 4 ·7H 2 O100mg was dissolved in 10mL sterilized distilled water and stored at -4°C.

[0037] DF+ACC medium: Add 3mM ACC to each liter of DF medium.

[0038] DF+ACC+Cd medium: add Cd to DF+ACC medium 2+ , the concentration is 0.5mM.

[0039] LB liquid medium: Each liter of culture medium contains tryptone 10g, yeast extract 5g, ...

Embodiment 2

[0044] The isolation and identification of embodiment 2 target bacterial strains

[0045] (1) culture medium

[0046] Enriched medium: KNO is contained in each liter of medium 3 10g, KCI1g, MgSO 4 ·7H 2 O0.5g, CaCl 2 1mg, FeSO 4 ·7H 2 O10mg, KH 2 PO 4 0.5g, 7.5g glucose, pH7.0, solvent is water, high pressure steam sterilization (121°C, 20min).

[0047] Separation medium: each liter of culture medium contains KN0 3 10g, KCI1g, MgSO 4 ·7H 2 O0.5g, CaCl 2 1mg, FeSO 4 ·7H 2 O10mg, KH 2 PO 4 0.5g, glucose 7.5g, agar 15.0g, pH7.0, solvent is water, high pressure steam sterilization (121°C, 20min).

[0048] Nitrate nitrogen conversion assay medium: each liter of culture medium contains KNO 3 1g, KCI1g, MgSO 4 ·7H 2 O0.5g, CaCl 2 1mg, FeSO 4 ·7H 2 O10mg, KH 2 PO 4 0.5g, 7.5g glucose, pH7.0, solvent is water, high pressure steam sterilization (121°C, 20min).

[0049] LB liquid medium: Each liter of culture medium contains tryptone 10g, yeast extract 5g, NaCl 1...

Embodiment 3

[0060] The identification of embodiment 3 target strain SAN1

[0061] (1) Observation of colony and cell morphology of SAN1 strain

[0062] Bacterial Morphological Study: Gram-stained bacteria were observed under an optical microscope with a 16×100 oil lens.

[0063] Research on the internal structure of the bacteria: Ultra-thin sections were made on the bacteria, and the internal structure of the bacteria was observed with a transmission electron microscope.

[0064] On solid medium, the young colonies of strain SAN1 were round, protruding, smooth and shiny, moist, translucent, viscous, and easy to pick. With the prolongation of culture time, the gloss of colony surface became darker and the color became light brown. Stained with basic fuchsin and observed under a microscope, most of the strains were blunt and round at both ends. Gram staining is positive, the bacteria are rod-shaped, the size is 2.5-3.3μm-1.3-2.1μm, producing spores, the spores are approximately columnar,...

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Abstract

The invention discloses an endophyte which is classified and named Bacillus megaterium and is completely named Bacillus megaterium SAN1. The endophyte is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March. 11, 2013 and has a preservation number of CGMCC No.7295. The invention also discloses an application of the endophyte in repair of facility soil secondary salinization and Cd combined pollution. The Bacillus megaterium CGMCC 7295 disclosed by the invention can be used for prompting growth of plants, improving physical and chemical properties of soil, promoting the plants to absorb Cd, effectively eliminating Cd pollution in the soil, efficiently converting nitrate nitrogen of soil and alleviating the hazards of secondary salinization; and due to combined action between the Bacillus megaterium CGMCC 7295 and the plants, the facility soil secondary salinization and the heavy metal Cd combined pollution can be repaired.

Description

technical field [0001] The invention belongs to the technical field of soil restoration, and in particular relates to a plant endophyte and its application. Background technique [0002] Facility cultivation is one of the important methods of vegetable production in my country. It has the advantages of high yield per unit area and little seasonal influence. It plays an important role in off-season and cross-regional planting of vegetables and other economic crops. However, under this production model with strong human intervention, high input and high output, due to the excessive use of agricultural chemicals, especially nitrogen fertilizers, and the application of unqualified cadmium-containing phosphorus fertilizers, nitrate and nitrite in the soil The problem of heavy metal Cd pollution has become prominent, leading to secondary salinization of facility soil and compound pollution of heavy metal Cd, seriously affecting the yield and quality of crops, and threatening the h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10B09C1/00A01G1/00A01G7/00A01N63/00A01P21/00C12R1/11
Inventor 冯英潘风山刘庆坡陈宝马晓晓杨肖娥
Owner ZHEJIANG UNIV
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