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Separation process for plant endophyte

A plant endophyte and endophyte technology, applied in the field of microbiology, can solve the problem of endophyte not being separated, and achieve the effect of good separation effect

Inactive Publication Date: 2009-05-20
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, during centrifugation, many endophytes may still be present in the plant tissue without being isolated

Method used

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  • Separation process for plant endophyte
  • Separation process for plant endophyte

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Two methods were used to separate the endophytes in the four plants of Nightshade Solanum, Dendrobium, Lemon 7, and Water Chicken; figure 1 Shown; Adopt the method of the present invention to separate endophyte flow process such as figure 2 shown.

[0027] Subsequently, the plates obtained by the above two methods were cultured at a constant temperature of 30°C for 2 to 14 days, and then different forms of colonies were picked, fungi were transferred to Chapeauer medium, bacteria were transferred to TSB solid medium, cultured by streaking until pure, and counted The results are shown in the table below:

[0028] root endophyte count stem endophyte count leaf endophyte count The total number of endophytes in Solanum nigrum Not using the method of the present invention 7 11 5 23 Adopt the method of the present invention 34 36 27 97

[0029] root endophyte count stem endophyte count leaf endophyte count The total...

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Abstract

The invention provides a method for obtaining plant endophytes by separation, and the method comprises the steps of plant pre-treatment, surface disinfection, leaching of the endophytes, separation and purification of the endophytes, and the like; compared with the ordinary method, the method not only utilizes mechanical grinding and shearing force to leach the plant endophytes, but also adds a surfactant and a cellulose enzyme to decompose plant fibers and plant cell walls, thereby leading the endophytes in plant tissues to be released more fully and obtaining better separation effect of the plant endophytes.

Description

technical field [0001] The invention belongs to the field of microbiology, and relates to a method for comprehensively separating and obtaining endophytic bacteria from plants Background technique [0002] Plant endophytes widely exist in various plants, and they have a huge impact on the life activities of the host. At present, the research on plant endophytes is becoming a hot spot in the fields of biology, environment, and chemistry in the world. The existing published plant endophyte isolation method 200710065724 mainly uses mechanical force grinding and centrifugation to separate plant endophyte from plant tissue homogeneity. However, during centrifugation, many endophytes may still be present in the plant tissue without being isolated. However, the research on plant endophytes is eager to obtain all the endophytes in the plant host as much as possible, and the method used in the present invention can obtain all the endophytes in the plant host to the greatest extent. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00
Inventor 罗胜联肖潇万勇陈亮郭函君曹喆苏峰
Owner HUNAN UNIV
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