Bacillus pumilus and application in preventing and treating dothiorella gregaria

A technology for Bacillus pumilus and poplar canker, which is applied in the directions of application, pest control, bacteria, etc. to achieve the effects of easy preservation, easy industrial production, and good development and application prospects.

Active Publication Date: 2010-02-10
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the prevention and treatment of poplar canker by Bacillus pumilus at home and abroad

Method used

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  • Bacillus pumilus and application in preventing and treating dothiorella gregaria
  • Bacillus pumilus and application in preventing and treating dothiorella gregaria
  • Bacillus pumilus and application in preventing and treating dothiorella gregaria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Antibacterial effect of Bacillus pumilus JK-SX001 on plates of poplar canker pathogens Cytosporachrysosperma, Phomnopsis sp., and Fusicoccum aesculi.

[0027] Inoculate the Bacillus pumilus JK-SX001 strain activated on the NA slant by streaking on both sides of the PDA plate, and then use a puncher with a diameter of 5 mm to punch out the same quality and amount of bacterial blocks from the PDA plate culture of the poplar canker pathogen. Point in the middle of the plate. Cultivate at 28°C for 4 days to observe the presence or absence of the inhibition zone, and record its width (Table 1). The test results showed that JK-SX001 had strong antagonistic effects on the three pathogenic bacteria.

[0028] Table 1 Inhibition of JK-SX001 on three kinds of poplar canker pathogens

[0029]

Embodiment 2

[0030] Embodiment 2: the metabolite of Bacillus pumilus JK-SX001 is to 3 kinds of poplar canker pathogens (comprising Phomopsis sp. (Phmnopsis sp.), golden shell cyst sp. The plate antibacterial effect of Fusicoccumaesculi)).

[0031] Spread sterile cellophane on the PDA plate, spread and inoculate Bacillus pumilus JK-SX001, incubate at 30°C for 48 hours, remove the cellophane, inoculate pathogenic bacteria in the center of the plate respectively, and incubate at 28°C. With no birth control bacteria control, after the control mycelia covered the plate, the antagonistic activity was observed, and the bacteriostatic rate was counted (Table 2). The test results showed that the metabolites of JK-SX001 had strong antagonistic effects on the three pathogenic bacteria.

[0032] Table 2 Inhibition of metabolites of JK-SX001 on three pathogenic bacteria

[0033]

Embodiment 3

[0034] Example 3: Growth-promoting effect of Bacillus pumilus JK-SX001 on 150-day-old Populus americana seedlings.

[0035] Bacillus pumilus JK-SX001 was inoculated in NB medium (3g of beef extract, 10g of peptone, 5g of sodium chloride, pH7.2-7.4, 1L of distilled water) and fermented for 48 hours. Sow the seeds after washing with bacteria water. The seeds were soaked in sterile water as a control. Seedling height 15cm Dingmiao, 4 plants per pot, measure seedling height and ground diameter after 150 days. Compared with the control, the inoculation of JK-SX001 fermentation broth can significantly promote the growth of seedlings (Table 3). Compared with the control (CK), the treatment inoculated with JK-SX001 had significant differences in ground stem and seedling height, and the growth promoting effect was obvious, see figure 1 .

[0036] Table 3 Effect of JK-SX001 on the growth of poplar seedlings

[0037]

[0038] Note: P<0.05

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Abstract

The invention discloses a bacillus pumilus JK-SX001 which is classified and named bacillus pumilus and collected in a China center for type culture collection (CCTCC) and has the collection number ofM 209026 on February 18th, 2009. The invention also discloses applications of the bacillus pumilus in preventing and treating dothiorella gregaria and promoting the growth of a poplar. The invention also provides a bactericide for preventing and treating the dothiorella gregaria, which is obtained by vaccinating the JK-SX001 in a culture medium to ferment and culture. The JK-SX001 is a plant endophyte, has obvious inhibiting effect on dothiorella gregaria, simple culture condition, easy storage and favorable development and application prospect and is easy for industrialized production.

Description

technical field [0001] The invention belongs to the field of plant protection, in particular to a bacillus pumilus and its application in preventing and treating poplar canker. Background technique [0002] Poplar is an important fast-growing timber species with high economic value and ecological benefits. However, poplars are constantly threatened by pests and diseases during their growth. Poplar canker is a serious disease of poplar branches and stems, which has many kinds of pathogens and various symptoms, so it is difficult to prevent and treat. [0003] At present, the control of poplar canker is mainly based on chemical control and breeding of resistant varieties. Although the method of disease-resistant breeding has achieved certain results, there are problems such as resistance degradation and low resistance; and the extensive use of chemical pesticides can easily cause environmental pollution and ecological balance damage. A large number of studies have shown tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00A01P21/00C12R1/07
Inventor 叶建仁任嘉红吴小芹王艳芳
Owner NANJING FORESTRY UNIV
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