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A nucleic acid-based method for tree phenotype prediction

A phenotypic and nucleic acid technology, applied in horticultural methods, botanical equipment and methods, and microbial determination/inspection, etc., can solve the problem of not giving the degree of hybridization

Inactive Publication Date: 2003-10-22
PULP & PAPER RES INST OF CANADA
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Problems solved by technology

But these probes give no information on the degree of hybridization in the introgressed clones

Method used

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  • A nucleic acid-based method for tree phenotype prediction
  • A nucleic acid-based method for tree phenotype prediction
  • A nucleic acid-based method for tree phenotype prediction

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[0064] The present invention provides a novel method for predicting selected phenotypes and rapidly selecting superior trees for known pulp and paper production lines using DNA probes. The method comprises the steps of isolating tree genome DNA from spruce living tissue, hybridizing the genome DNA with a DNA probe, and evaluating the intensity of the hybridization pattern obtained by optical density.

[0065] A particular embodiment of the present invention is to determine the exact extent of genetic admixture (or introgression) of two parental species in a hybrid population. Due to - for example in hybrid spruce populations - the linear relationship between the degree of genetic introgression and fiber length (for the purposes of the present invention), the strength of the DNA probe hybridization profile can be used to directly, precisely and reproducibly Predict the fiber length of individual Picea spruce / interoir spruce hybrids (for a given tree age) in a population.

[00...

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Abstract

The present invention relates to a novel method for the prediction of fibre length and the rapid selection of superior trees for given pulp and paper product lines using a DNA probe. The method comprises the isolation of tree genomic DNA from a hybrid spruce live tissue source, hybridization of the spruce DNA probe to that genomic DNA and the densitometric assessment of the intensity of the hybridization pattern obtained. This determines the precise degree of genetic admixing (or introgression) of the two parent species within the hybrid population. Due to the linear relationship - in the hybrid spruce population examined - between degree of genetic introgression and fibre length (discovered in this method), the intensity of the DNA probe hybridization pattern can be used to directly, accurately and reproducibly predict the fibre length found (for a given tree age) within an individual hybrid spruce within the population.

Description

Background of the invention [0001] a) Field of invention [0002] The present invention belongs to the fields of molecular biology and tree improvement, pulp and paper property evaluation, and the present invention can more effectively screen trees with given fiber properties from natural and planted tree populations for specific pulp and paper production lines. [0003] B) Background technology [0004] For a long time, introgression (a natural form of genetic crossing) was thought to be common in the spruce species Picea sitchensis, P. glauca and Engelmann's spruce (P. .engelmannii) (Daubenmire, R. (1968) Can. J. Bot, 46:787-798). The region where this hybridization occurs is known as the "introgression zone," an example of which can be found in the Nass Skeena transition in British Columbia (Fig. 1). Two major groups of such natural hybrids occur in this belt: the "interior" hybrid (which is primarily a mixture of white spruce and Engermann's spruce) and the interior and...

Claims

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Application Information

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IPC IPC(8): A01H1/04A01H7/00C12Q1/68C12Q1/683C12Q1/6895
CPCC12Q1/683C12Q1/6895A01H7/00A01H1/04C12Q2600/13C12Q2600/156
Inventor S·泊特P·A·沃特森
Owner PULP & PAPER RES INST OF CANADA
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