67 results about "Pinus cembroides" patented technology

The invention relates to an emamectin benzoate injection to prevent pine wilt disease, which is characterized in that, the components of the invention are 0.5% to 2% emamectin benzoate, 10% to 15% tween 80 or tween 60, 15% to 20% butyl glycol ether and 65% to 72% organic solvent; the components are arranged in an injection container; the percentage is mass percentage. The emamectin benzoate injection has the advantages that, compatibility composition is more reasonable and more pertinent; through proper surface-active material and addition agent, the conduction of emamectin benzoate in pines is easier, therefore pine wood nematode can be eliminated; the form of the pharmaceutical preparation is more reasonable and more scientific; the invention has convenient use and practicality; active substance is added; combined with injection, the invention has better killing effect.

The present invention relates to improved pine grafting technology. In the vigorous growth period after pine fluid flowing, the cold stored scion has the terminal bud bottom cut into wedge, the anvil has the terminal bud cut off and one central cut formed for the scion to be inserted, the cambia of both the anvil and the scion are made to contact closely, and the grafted part is tied and fastened with tying material. The technological scheme of terminal bud wedge grafting is superior to available pine grafting technology, and has high survival rate and high average growth amount.

A method for in vitro culturing an adventitious bud by a Korean larch to induce a plant regeneration relates to a method for in vitro culturing the adventitious bud by pine to induce the plant regeneration which solves the problems of difficult generation of the tissue culture organs of the Korean larch, low plant regeneration rate and low inducing rate by using a mature embryo, a flower bud and axillary bud, as well as a tender stem segment of the Korean larch as an explant adventitious bud currently. The method relates to: 1. Culturing and inducing a callus by a zygotic embryo; 2. Inducing to generate the adventitious bud; 3. Continuously culturing and inducing to root, namely acquiring the regeneration plant in vitro of the Korean larch. The invention induces the adventitious bud by taking a mature seed of the Korean larch as the explant and acquires the adventitious bud of high inducing rate which is improved by more than 9 times than the currently existing adventitious bud inducing technology and the inducing rate of the callus is all higher than 90 percent, which has a wide application value on the massive expanding propagation and gene engineering breading research of the Korean larch.

The invention discloses a high-efficiency phytate degradation bacterium Rahnella aquatils and an application thereof in prompting plant growth. The classification naming of the high-efficiency phytate degradation bacterium is Rahnella aquatils JZ-GX1 which is preserved in the China Center for Type Culture Collection, the preservation number is CCTCCNO: M2012439, the preservation date is, November 6th, 2012, and the preservation address is Wuhan, China. The Rahnella aquatils JZ-GX1 disclosed by the invention has higher phytase activity, can effectively degrade slightly solubility phytate such as calcium phytic acid and the like; and the bacterium also can produce phytohormone IAA. A series of growth prompting tests show that compared with a reference phase, the bacterium has a significant prompting function to the sprouting and growth of corn and the growth of poplar trees and piney. Excellent bacterium resources are provided for biological bacterial fertilizers for plants such as corn, poplar trees and piney.

A process for earlierly detecting pine nematosis includes inoculating the disc fungus (Ps0202) or other microbial fungi on culture medium, drilling circular hole on the pine trunk to be detected, inserting the test tube containing said fungi in said hole, staying it in the hole for 3-5 days, and determining by microscope. Its advantages are easy operation and high sensitivity.

The invention relates to an abamectin injection to prevent pine wilt disease, which is characterized in that, the components of the invention are 2% to 4% abamectin, 10% to 15% tween 80 or tween 60, 15% to 20% butyl glycol ether and 68% to 72% organic solvent; the components are arranged in an injection container; the percentage is mass percentage. The abamectin injection has the advantages that, compatibility composition is more reasonable and more pertinent; through proper surface-active material and addition agent, the conduction of abamectin in pines is easier, therefore pine wood nematode can be eliminated; the form of the pharmaceutical preparation is more reasonable and more scientific; the invention has convenient use and practicality; active substance is added; combined with injection, the invention has better killing effect.

The invention relates to a large-scale planting method of organic polygonatum kingianurn under an artificial pine forest. The method includes the steps of selecting the artificial pine forest suitablefor polygonatum kingianurn growth and with the soil environment quality, irrigation water quality and environment air quality conforming to the national standards to plant the polygonatum kingianurn,applying rotten farmyard manure as a base fertilizer, keeping the under-forest light permeability at 25-30%, covering the ground for planting the polygonatum kingianurn with pine needles, keeping thesoil water content at 25% or above, preventing and treating the leaf spot disease or tan disease with a copper hydroxide solution, pouring a Bordeaux mixture on roots to prevent and treat the root rot, planting Chinese cabbage on the edges of a forest land to attract injurious insects (scarab or agrotis ypsilon) to eat, pouring a radix sophorae flavescentis alkaline water agent on roots to prevent and treat larvae, and inducing and killing adult insects through venom prepared by mixing sweet and sour liquid and a radix sophorae flavescentis alkaline water agent. The organic production aims for the environment and polygonatum kingianurn product quality safety are realized, the organic production cost is reduced, and the organic product quality is improved.

The invention discloses a pinus massoniana cpSSR polymorphism primer and an identification method of pine sibling species of the primer. According to design of a chloroplast genome of pinus massoniana, 16 pairs of cpSSR primers are obtained, further, 7 pairs of primers which are stable in difference and good in polymorphism are screened from the 16 pairs of cpSSR primers, and through utilization of the 7 pairs of primers, a finger print and phylogenetic tree of 8 kinds of pine sibling species are successfully established. Based on the 7 pairs of cpSSR primers, a PCR amplification reaction is carried out. Capillary electrophoresis technology is used to analyze a PCR product, 8 kinds of pine sibling species trees can be accurately identified. The primer and method are efficient, accurate, and convenient and are not prone to be affected by the environment. The polymorphism primer and an identification method thereof can reflect the genetic information of the 8 kinds of pine plasmons, andcan be used for research of identification of 8 kinds of pine sibling species and genetic relationship.

The invention relates to a preparing method of a masson pine seedling culture medium. The preparing process includes the steps that 50-60 parts of waste snake house packing, 25-40 parts of chinese mesona herb residues, 10-25 parts of manioc residues, 10-20 parts of straw pellets, 5-10 parts of ramulus mori pellets, 3-8 parts of cob residue pellets and 3-5 parts of bagasse pellets are weighed by weight; the raw materials are mixed uniformly to obtain a premix compound; mixed bacteria are added into the premix compound; the premix compound and the mixed bacteria are mixed uniformly and then putinto a tunnel fermenting kiln to conduct fermentation; pasteurization is conducted on the premix compound obtained after fermentation to obtain culture material; the culture material obtained after pasteurization is placed in a edible fungi cultivation shed to be cooled to 25 DEG C or below, edible fungus strains are planted to conduct edible fungus cultivation; after edible fungus cultivation isfinished, masson pine branch and leaf pellets are added, the mixture is placed for one week or more and turned then to obtain the masson pine seedling culture medium. Masson pines cultivated by the seedling culture medium are high in survival rate and have developed lateral and fibrous roots.

The invention provides an attractant for trapping shondylis buprestoides linne imagoes, which comprises the following components in percentage by volume: 7 to 8 percent of (1r)-(+)-alpha-pinene, 7 to 8 percent of (1s)-(-)-alpha-pinene, 4 to 5 percent of (1s)-(-)-beta-pinene, 22 to 23 percent of beta-myrcene, 31 to 33 percent of R-(+)-limonene and the balance of absolute ethanol. The attractant has the strong inducing effect on shondylis buprestoides linne, inducing active substances in the attractant are released gradually by a sustained-release bottle, so the lasting period can reach 1 to 2 months. When used in combination with a black plastic cross-shaped trapper, the attractant can well trap the shondylis buprestoides linne imagoes. In addition, the attractant also has the characteristics of strong environmental-friendly character, low cost and simple preparation process, can be used for trapping the shondylis buprestoides linne imagoes, controlling the woodland population density of the shondylis buprestoides linne and inhibiting the propagation and contagion of nematode diseases of pine trees effectively, and provides the important technical and product support for the comprehensive treatment of the nematode diseases of the pine trees and the shondylis buprestoides linne imagoes.

The invention discloses a strain of bacillus cereus HB12 ectotrophic mycorrhiza helper bacteria, which belongs to the class of bacillus cereus and has been collected in China Center for Type Culture Collection (CCTCC) with a collection number of CCTCC No.M 209025 on February 18th, 2009. The bacillus cereus HB12 is a plant growth-promoting rhizobacterium and is capable of promoting the growth of black tree and the growth of boletus edulis ectotrophic mycorrhiza. When the bacillus cereus HB12 is inoculated to black pine together with the boletus edulis, the growth-promoting effect produced is more desirable than those achieved by the respective inoculation of the bacillus cereus HB12 and the boletus edulis; the bacillus cereus HB12 can inhibit pathogenic rhizoctonia of pine seedling damping-off; and green house prevention and control experiments show that the bacillus cereus HB12 can prevent and control pine seedling damping-off effectively.

The composition and process for preparing an anti-anal fistula composition which comprises providing a predetermined effective amount of natural substances of the genera Safflower, Notoginseng Radix, Lightyellow Sophora, Elecampane Redix, Rumex, Pine Resin, Sassafras Tree, Glycyrrhizae Radix, Testudinis Carapax, Rhei Rhizoma, Ephedrae Herba, Natrii Sulfas, Menthae Herba, Pasoniae Radix, Acontii Tuber, Corni Fructus, Gypsum, and Pelladendri Radix in an aqueous medium to form an initial mixture, extracting the initial mixture with water at a temperature of about 65°-75° C. for 1-2 hours to produce an aqueous mixture, filtering the aqueous mixture to produce a filtrate, and evaporating the filtrate to a moisture content of 30% to produce an extract which can be used as a treatment for hemorrhoid diseases including anal fistula.

The invention relates to a synergist special for edible fungus and a preparation and use method thereof. The synergist special for the edible fungus comprises the following compositions by weight portion: 40 to 70 portions of treated fowl manure, 10 to 25 portions of calcareous shale, 5 to 35 portions of wheat bran and 5 to 10 portions of lime. The method comprises: adding 5 to 30 percent of pine needles and 0.1 to 0.5 percent of fermenting agent for deodorization according to the weight of dry fowl manure; adding water and uniformly mixing manure materials to control the water content of the manure materials to be between 55 and 65 percent, and performing stacking fermentation for 15 to 25 days at natural temperature; drying fermented fowl manure until the water content is less than 20 percent; and uniformly mixing the four compositions to synthesize the synergist. The amount of the synergist accounts for 10 to 30 percent of the weight of edible fungus cultivated materials, and the water content of the cultivated materials is between 55 and 60 percent. The synergist not only reduces the production cost and improves the yield of edible fungus but also reduces environmental pollution of the fowl manure, is favorable for normal growth and metabolism of hypha or fruiting bodies, has no toxicity, harm and potential accumulated toxicity on human beings and animals, and can be widely used for cultivating edible fungus such as oyster mushrooms, yellow oyster mushrooms, chicken leg mushrooms and branched oyster mushrooms.

The present invention relates to a strain of efficient plant growth promoting bacteria Bacillus subtilis, and a low cost production method and a use method of a bacterial agent of the plant growth promoting bacteria Bacillus subtilis, and belongs to the technical field of microbial fertilizers and energy-saving emission reduction. According to the present invention, the used strain is a strain Bacillus subtilis SZX11 separated and screened from pine tree leaf blade and having a plant growth promoting function, is preserved in the China General Microbiological Culture Collection Center, and has the preservation number of CGMCC No.8188; sweet potato starch wastewater is adopted as a liquid-state fermentation culture medium to carry out large-scale cultivation of Bacillus subtilis and prepare the agricultural microbial agent, such that the energy consumption of the process is low, the production cost is low, the operation is simple, the sweet potato starch can be subjected to resource utilization, and the pollution emission can be reduced; and with the application of the produced bacterial agent in the tea leaf and vegetable production, the yield can be significantly increased, the quality of the agricultural products can be significantly improved, and the broad application prospects are provided.

The invention discloses a tank culture method for dendrobium officinale. A culture tank is made of hot galvanized iron wires and filled with pine bark as a culture matrix, dendrobium officinale seedlings in season are planted, and the culture tank is arranged in a suitable culture place (for example, at the lower part of a forest tree, in a culture facility or on a balcony roof with a sunshade facility), so that simple culture of the dendrobium officinale is realized. The invention provides a new choice for culturing the dendrobium officinale; compared with other culture methods, the tank culture method has the characteristics of simplicity for operation, convenience for management and protection, flexibility and low planting cost; a finite space can be fully utilized; a culture position is flexibly adjusted according to the change of environmental factors, so that the growth of the dendrobium officinale is facilitated, the occurrence and development of pest and disease damage are prevented, and the use of pesticides can be reduced; and the tank culture method has a promotional effect on popularization of under-forest scattered planting and family planting of the dendrobium officinale.

The present invention relates to methods for the selection of transformed embryogenic tissue of coniferous plants. In particular, the invention relates to improved methods for selecting transformed embryogenic tissue of plants of the subgenus Pinus of pines, particularly Southern yellow pines and hybrids thereof using an agent that regulates differentiation of embryos from embryogenic cells.

The invention discloses a dendrobium cultivating medium. The dendrobium cultivating medium is prepared from pinecones, wood shavings and saw dust in a mixed mode, wherein the volume of the pinecones accounts for 50% to 70% of the total volume of the medium, the volume of the wood shavings accounts for 20% to 30% of the total volume of the medium, and the volume of the saw dust accounts for 10% to 20% of the total volume of the medium. The pinecones need to be subjected to early-stage processing; in other words, the pinecones need to be sterilized under the blazing sun for quite a long time or through boiling at high temperature; the wood shavings and the saw dust must be wood shavings and saw dust of pine trees or cedars and also must be subjected to early-stage processing. According to the dendrobium cultivating medium, in the main raw materials, the pinecones are waste in a pine forest area, the wood shavings and the saw dust are waste of a wood-working factory, all the raw materials can be obtained in a large-scale mode, the pinecones are not prone to rotting or generating insect damage, the service life of the medium is prolonged, and the cost of the medium is reduced; in addition, the air permeability of the pinecones is good, the good moisture preserving and fertilizer preserving characteristics are achieved, after the pinecones, the wood shavings and the saw dust are mixed to be used, the plant fixing requirement can be guaranteed, dendrobium grows robustly, the survival rate is high, the root system is well developed, and the yield is high.