High-efficiency phytate degradation bacterium Rahnella aquatils and application thereof in prompting plant growth

A technology of Lahenia water and phytate, which is applied in the field of microorganisms to achieve the effect of strong phytase activity, excellent strain resources, and growth promotion.

Active Publication Date: 2013-05-29
NANJING FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few domestic reports on the screening of efficient phytate-degrading bacteria in

Method used

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  • High-efficiency phytate degradation bacterium Rahnella aquatils and application thereof in prompting plant growth
  • High-efficiency phytate degradation bacterium Rahnella aquatils and application thereof in prompting plant growth
  • High-efficiency phytate degradation bacterium Rahnella aquatils and application thereof in prompting plant growth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Determination of phytate degradation ability of Lahnella water JZ-GX1.

[0025] Phytate solid screening medium: Glucose 10g, Phytin 4.0g, MgCl 2 ·6H 2 O 5.0g, MgSO 4 ·7H 2 O 0.25g, KCl 0.2g, (NH 4 ) 2 SO 4 0.1g, 18.0g agar, 1000mL distilled water, adjust the pH to 7.0 with 0.1M NaOH solution.

[0026] Spot the activated JZ-GX1 strain and the control strain CK (organophosphate-solving bacteria YY-SX1, which was screened and preserved in the laboratory) on the phytate solid screening medium plate, and place the plate in a constant temperature incubator at 28°C were cultured for three days, with 3 replicates for each treatment. Then measure the colony diameter, penetrating ring and colony diameter of each bacterial strain, calculate the ratio, and name the ratio as HE.

[0027] The results are shown in Table 1 and figure 1 As shown, it can be preliminarily judged that the strain JZ-GX1 has better phytate degradation ability, which is significantly highe...

Embodiment 2

[0031] Example 2 Determination of phytase activity of Lahnella water JZ-GX1.

[0032]NB medium: beef extract 3g, peptone 10g, sodium chloride 5g, distilled water 1000mL, pH 7.2~7.4.

[0033] Phytate liquid fermentation medium: except that agar is not added, other components are the same as phytate solid screening medium.

[0034] Inoculate the activated JZ-GX1 strain and the control strain (YY-SX1, an inorganic phosphate-solving bacterium YY-SX1, which was screened and preserved in the laboratory) into medium NB medium, culture at 30°C and shake at 180r / min for 18-24 hours to make a seed solution, and take 0.5mL seed solution was inoculated into 100mL Erlenmeyer flasks containing 50mL phytate liquid fermentation medium, and each treatment was repeated three times. After 72 hours of shaking culture at 30°C and 180r / min, the fermentation broth was centrifuged at 4°C and 10000r / min for 10min. , Take the supernatant, that is, the crude enzyme solution, to measure the phytase acti...

Embodiment 3

[0036] Example 3 Determination test of auxin IAA produced by Lahnella water JZ-GX1.

[0037] TSB medium: tryptone 15g, soybean peptone 5g, NaCl 5g, distilled water 1L, pH7.2~7.4.

[0038] Add 50mL of TSB culture solution to each shake flask, and then add L-tryptophan to the concentration of 0, 100, 200, 300 and 500μg / mL (L-tryptophan mother solution: prepare with distilled water at 30-40℃ to 2 mg / mL, sterilized with a bacterial filter and stored for later use).

[0039] Inoculate the activated JZ-GX1 strain into NB medium, and culture at 30°C with shaking at 180r / min for 18-24 hours to make a seed solution. Take 0.5mL of the seed solution and inoculate them into 100mL Erlenmeyer flasks containing 50mL of TSB medium. Each treatment was repeated three times, cultured with shaking at 30°C and 180r / min, and the content of auxin IAA produced was measured after 72 hours. The fermentation broth was centrifuged at 5000r / min for 20min at 4°C to remove bacteria, and 1mL supernatant wa...

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Abstract

The invention discloses a high-efficiency phytate degradation bacterium Rahnella aquatils and an application thereof in prompting plant growth. The classification naming of the high-efficiency phytate degradation bacterium is Rahnella aquatils JZ-GX1 which is preserved in the China Center for Type Culture Collection, the preservation number is CCTCCNO: M2012439, the preservation date is, November 6th, 2012, and the preservation address is Wuhan, China. The Rahnella aquatils JZ-GX1 disclosed by the invention has higher phytase activity, can effectively degrade slightly solubility phytate such as calcium phytic acid and the like; and the bacterium also can produce phytohormone IAA. A series of growth prompting tests show that compared with a reference phase, the bacterium has a significant prompting function to the sprouting and growth of corn and the growth of poplar trees and piney. Excellent bacterium resources are provided for biological bacterial fertilizers for plants such as corn, poplar trees and piney.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a high-efficiency phytate-degrading bacterial strain Lahnella water JZ-GX1 screened from the rhizosphere soil of Pinus massoniana and its effect on promoting the germination and growth of corn and the growth of poplar and pine application. Background technique [0002] Phosphorus is the second largest nutrient element necessary for plant growth. For plants, they can directly obtain nitrogen from the air by using biological nitrogen fixation, while phosphorus must be obtained through the application of phosphorus fertilizers. Since most of the soils in the world suffer from shortage of phosphorus fertilizer supply, chemical phosphorus fertilizers are applied to the soil every year to alleviate the shortage of phosphorus fertilizer supply. Although a large amount of chemical phosphorus fertilizers are applied, most of them are quickly converted into chelates that cann...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P21/00C12R1/01
Inventor 吴小芹李桂娥叶建仁侯亮
Owner NANJING FORESTRY UNIV
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