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Bacillus cereus ectotrophic mycorrhiza helper bacteria and use thereof

A technology of Bacillus cereus and ectomycorrhizal, applied in the direction of application, bacteria, biocide, etc.

Active Publication Date: 2010-12-15
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on the interaction between MHB and mycorrhizal fungi in foreign countries has a history of several decades, and a large number of MHBs have been screened out, and some excellent MHBs have been studied in depth. See screening studies on MHB and related reports on the interaction between MHB and mycorrhizal fungi

Method used

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  • Bacillus cereus ectotrophic mycorrhiza helper bacteria and use thereof
  • Bacillus cereus ectotrophic mycorrhiza helper bacteria and use thereof
  • Bacillus cereus ectotrophic mycorrhiza helper bacteria and use thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1: the growth-promoting effect of bacillus cereus HB12 to boletus deliciosa (Be)

[0025] After activating the bacteria to be tested, use an inoculation loop to inoculate a small amount into a 100mL Erlenmeyer flask containing 50mL TSA liquid medium, and incubate at 28°C with shaking at 160r / min for 72h. Pour the bacterial suspension into a sterilized centrifuge tube, centrifuge at 5000r / min at 4°C for 5min, pour off the supernatant, then wash the bacteria twice with normal saline, and finally add physiological Saline, after mixing, it will be the bacterial cell suspension of HB12 strain (containing about 5×10 8 cfu / mL). At the same time, draw the supernatant with a sterile syringe, and filter it with a bacterial filter (the filter membrane has a pore size of 0.22 μm and a diameter of 0.25 mm), which is the sterile filtrate of the HB12 bacterial strain.

[0026] (1) Determination of growth-promoting effect of Bacillus cereus HB12 cell suspension on boletus ...

Embodiment 2

[0039] Example 2: The growth-promoting effect of the interaction between Bacillus cereus HB12 and Boletus deliciosa (Be) on black pine.

[0040] Cultivation of sprouts: Black pine seeds are soaked in warm water at 60°C for 24 hours to accelerate germination, soaked in 0.5% potassium permanganate solution, sterilized for 2 hours, rinsed with tap water for 1 hour, sown in sterilized sand, and placed in 25 Cultivate in a greenhouse at ℃, and cut the roots and transplant the seedlings when they grow to the cotyledon stage.

[0041] Potted substrate: The substrate soil was collected from the back hill of Nanjing Forestry University campus, and the soil sample was sieved by 1.01×10 6 Pa sterilize for 90 minutes, cool down and set aside.

[0042] Preparation of Bacillus cereus HB12 bacterial cell suspension: After activating the test bacteria, use an inoculation loop to inoculate a small amount into a 100mL Erlenmeyer flask containing 50mL TSA liquid medium, culture at 28°C and shak...

Embodiment 3

[0048] Embodiment 3: bacillus cereus HB12 is to rhizoctonia (Rhizoctonia sp.) plate bacteriostasis

[0049] Inoculate the Bacillus cereus HB12 strain activated on the NA slant by streaking on both sides of the PDA plate, and then use a puncher with a diameter of 5mm to punch out the same quality and the same amount of bacterial blocks from the PDA plate culture of the pine seedling damping-off disease pathogen. Point in the middle of the plate. Incubate at 28°C for 7 days to observe the presence or absence of the inhibition zone, and record its width. From Table 5 and Figure 4 It can be seen that HB12 has an antagonistic effect on the pathogenic mycobacteria Rhizoctonia sp. Compared with the control, the bacteriostatic rate reached 44.2%.

[0050] Table 5 Inhibition of Bacillus cereus HB12 to Rhizoctonia sp.

[0051]

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Abstract

The invention discloses a strain of bacillus cereus HB12 ectotrophic mycorrhiza helper bacteria, which belongs to the class of bacillus cereus and has been collected in China Center for Type Culture Collection (CCTCC) with a collection number of CCTCC No.M 209025 on February 18th, 2009. The bacillus cereus HB12 is a plant growth-promoting rhizobacterium and is capable of promoting the growth of black tree and the growth of boletus edulis ectotrophic mycorrhiza. When the bacillus cereus HB12 is inoculated to black pine together with the boletus edulis, the growth-promoting effect produced is more desirable than those achieved by the respective inoculation of the bacillus cereus HB12 and the boletus edulis; the bacillus cereus HB12 can inhibit pathogenic rhizoctonia of pine seedling damping-off; and green house prevention and control experiments show that the bacillus cereus HB12 can prevent and control pine seedling damping-off effectively.

Description

technical field [0001] The invention belongs to the field of microbial fertilizers, and in particular relates to an ectomycorrhizal auxiliary bacterium bacillus cereus and its application in promoting the growth of black pine and preventing and controlling pine seedling damping-off. Background technique [0002] Plant growth-promoting rhizobacteria (PGPR) is a general term for bacteria in the rhizosphere microflora that can promote plant growth, prevent disease, and increase crop yield. Since Burr et al. first reported PGPR on potatoes in 1978, more than 20 species of PGPR have been discovered at home and abroad. Among them, Pseudomonas, Bacillus, Agrobacterium, Eriwinia, Flavobacterium, Pasteuria , Serratia (Serratia), Enterobacter (Enterobacter) and so on are the most prominent. Plant rhizosphere growth-promoting bacteria and ectomycorrhizal fungi (Ectomycorrhizal Fungi, EMF), as an important group of plant rhizosphere microorganisms, play an important role in plant nutr...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00A01P21/00C05F11/08C12R1/085
Inventor 吴小芹盛江梅侯亮亮叶建仁刘辉
Owner NANJING FORESTRY UNIV
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