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Plant growth promoting bacteria separated from phyllosphere, and production method of bacterial agent of plant growth promoting bacteria

A technology of plant pathogenic bacteria and microbial agents, applied in plant growth regulators, methods based on microorganisms, biochemical equipment and methods, etc., can solve problems such as improper treatment, black and smelly water quality, and large energy consumption

Active Publication Date: 2016-06-01
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current processing technology produces about 10 tons of high-concentration organic wastewater for every 1 ton of sweet potato starch produced. Among them, the sweet potato cell fluid after starch separation has the highest organic content, and the COD can reach more than 20,000 mg / L, mainly containing soluble starch. , protein, pectin, organic acid and a small amount of oil, if not handled properly, it is easy to be spoiled and fermented, making the water black and smelly
However, the general sewage treatment process is used to treat such high-concentration wastewater to meet the standard discharge, and the energy consumption is very large.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: sweet potato starch waste water produces Bacillus subtilis microbial bacterial agent

[0019] (1) sweet potato starch wastewater pretreatment: in high-concentration sweet potato starch wastewater, add 0.5% plant ash (from the solid waste of biomass thermal power plant, its main component is potassium carbonate) according to weight ratio, repeat mixing, and then use lime The pH of the wastewater was adjusted to 8.0 with water and microwaved at 100°C for 5 minutes.

[0020] (2) Liquid seed preparation: inoculate the Bacillus subtilis preserved on the slant into a triangular flask containing pretreated sweet potato starch wastewater, and vibrate at 50° C. at 150 rpm for 28 hours to obtain liquid seeds.

[0021] 3. Liquid fermentation: inoculate the liquid seeds with a volume ratio of 5% in the pretreated sweet potato starch waste water fermenter tank, at 45°C, cultivate in sterile air for 16 hours; The number of viable bacteria reached 2×10 9 CFU / ml or more...

Embodiment 2

[0023] Embodiment 2: Bacillus subtilis microbial inoculum is applied to the production of organic tea

[0024]In the organic tea garden, choose about 2 acres of experimental land with uniform growth of tea trees. The management of the experimental tea trees is the same as that of other tea trees in the base, but the fertilization is different. The test site is divided into 5 blocks, corresponding to 5 treatments respectively. There is an isolation row of about 2 meters between each treatment. The bacterial agent is diluted with tap water or the fermentation broth is directly used. The 5 treatments are respectively clean water control (CK), starch wastewater control (FCK), high concentration (2×10 7 CFU / ml) spraying (H), medium concentration (1×10 7 CFU / ml) spraying (M), low concentration (5×10 6 CFU / ml) spraying (L), according to the amount of 2 liters of bacterial agent dilution per square meter. In the same way, each treatment was applied 3 times inoculum in winter, and t...

Embodiment 3

[0025] Example 3: Organic fertilizer rich in Bacillus subtilis is applied to tea production

[0026] In the tea garden, choose a test site with uniform growth of tea trees, and divide it into 6 plots, each plot is about 200 square meters. 7 CFU / gram) of organic fertilizer 3 treatments, each treatment 2 in parallel, each treatment is provided with an isolation row of about 2 meters, the amount of organic fertilizer and organic fertilizer rich in Bacillus subtilis is 1 ton per mu , fertilized once before winter, and harvested tea leaves in spring. The results showed that the yield of spring tea treated with organic fertilizer increased by about 15% compared with the control, while the yield of spring tea treated with bio-organic fertilizer increased by more than 35% compared with the control. The contents of water extracts, tea polyphenols and amino acids in the tea leaves of bio-organic fertilizers were significantly higher than those of the control.

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Abstract

The present invention relates to a strain of efficient plant growth promoting bacteria Bacillus subtilis, and a low cost production method and a use method of a bacterial agent of the plant growth promoting bacteria Bacillus subtilis, and belongs to the technical field of microbial fertilizers and energy-saving emission reduction. According to the present invention, the used strain is a strain Bacillus subtilis SZX11 separated and screened from pine tree leaf blade and having a plant growth promoting function, is preserved in the China General Microbiological Culture Collection Center, and has the preservation number of CGMCC No.8188; sweet potato starch wastewater is adopted as a liquid-state fermentation culture medium to carry out large-scale cultivation of Bacillus subtilis and prepare the agricultural microbial agent, such that the energy consumption of the process is low, the production cost is low, the operation is simple, the sweet potato starch can be subjected to resource utilization, and the pollution emission can be reduced; and with the application of the produced bacterial agent in the tea leaf and vegetable production, the yield can be significantly increased, the quality of the agricultural products can be significantly improved, and the broad application prospects are provided.

Description

Technical field: [0001] The invention relates to a high-efficiency plant growth-promoting bacterium (Bacillus subtilis SZX11 strain) isolated from pine leaves, and a low-cost production method of the bacterial agent, belonging to the technical field of microbial fertilizer and energy saving and emission reduction. Background technique: [0002] Bacillus subtilis (Bacillus subtilis) is a kind of aerobic type, endogenous rod-shaped bacteria resistant to reverse spores, the size is (0.8 ~ 1.2) μm × (1.5 ~ 4.0) μm, Gram staining is positive, can produce capsules, spores Mesozoic or near-mesobiotic, with rough colonies, capable of liquefying gelatin, peptonizing milk, reducing nitrate, hydrolyzing starch, typical aerobic bacteria that widely exist in soil, lakes, oceans, and animal and plant surfaces, and have no pathogenicity themselves . Bacillus subtilis can successfully colonize the rhizosphere, body surface or body of plants, compete with pathogenic bacteria for nutrients a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08A01P1/00A01P3/00A01P21/00C12R1/125
Inventor 白志辉梁胜贤张倩张乙铭庞晓丹庄国强
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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