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Preparation method of dendrobium candidum culture substrate

A cultivation medium and technology of Dendrobium officinale, applied in botany equipment and methods, cultivation, culture medium, etc., can solve the problems of low survival rate of tissue culture seedling transplanting, and achieve high transplanting, growth promotion and high survival rate Effect

Inactive Publication Date: 2017-03-29
巫溪县琼海农业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the development of tissue culture technology, artificial planting of Dendrobium officinale has become possible, but the survival rate of its tissue culture seedlings is low

Method used

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  • Preparation method of dendrobium candidum culture substrate

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A kind of preparation method of Dendrobium candidum cultivation substrate, its concrete steps are:

[0024] S1. Pine bark pretreatment: pine bark is placed in water (doubling the mass of pine bark) and soaked for 4 days and then disinfected (soaked in a solution diluted 800 times with 50% thiophanate-methyl wettable powder for 2-3 hours) , and then crushed into pine bark particles with a diameter of 1.0cm and pine bark particles with a diameter of 0.5cm;

[0025] S2. Coconut shell and sheep manure pretreatment: Coconut shell and sheep manure are respectively sterilized (soaked in a solution diluted 800 times with 50% thiophanate-methyl WP for 2 hours), aerobic fermentation (control temperature is 35°C, The relative humidity of the air is 55%, the pressure inside the fermentation tank is 0.025MPa, and the air volume is 750m 3 / h, the fermentation time is 5 days), then the product after coconut shell fermentation is pulverized into the coconut shell particle that diamete...

Embodiment 2

[0031] A kind of preparation method of Dendrobium candidum cultivation substrate, its concrete steps are:

[0032] S1. Pine bark pretreatment: pine bark is placed in water (consumption is 3 times of pine bark quality) and disinfected after soaking for 7 days (in the solution diluted 1200 times of 50% thiophanate-methyl wettable powder for 3h), then Be pulverized into the pine bark particle that diameter is 0.81cm and the pine bark particle that diameter is 0.8cm;

[0033] S2. Coconut shell and sheep manure pretreatment: Coconut shell and sheep manure are respectively sterilized (soaked in a solution diluted 1200 times with 50% thiophanate-methyl WP for 3 hours), aerobic fermentation (control temperature is 38°C, The relative humidity of the air is 52%, the pressure inside the fermentation tank is 0.021MPa, and the air volume is 770m 3 / h, the fermentation time is 8 days), then the product after coconut shell fermentation is pulverized into the coconut shell particle that diam...

Embodiment 3

[0039] A kind of preparation method of Dendrobium candidum cultivation substrate, its concrete steps are:

[0040] S1. Pine bark pretreatment: pine bark is placed in water (consumption is 2.5 times of pine bark quality) and sterilized after soaking for 5 days (soaking 2.5h in a solution diluted 1000 times with 50% thiophanate-methyl wettable powder), Then be pulverized into the pine bark particle that diameter is 0.95cm and the pine bark particle that diameter is 0.63cm;

[0041] S2. Coconut shell and sheep manure pretreatment: Coconut shell and sheep manure are sterilized respectively (soaked in a solution diluted 1000 times with 50% thiophanate-methyl WP for 2.5 hours), aerobic fermentation (control temperature is 36°C) , the relative humidity of the air is 54%, the pressure inside the fermenter is 0.023MPa, and the air volume is 760m 3 / h, the fermentation time is 6 days), then the product after coconut shell fermentation is pulverized into the coconut shell particle that ...

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Abstract

The invention belongs to the technical field of planting, and particularly relates to a preparation method of a dendrobium candidum culture substrate. The preparation method comprises the following specific steps: S1, pretreating pine bark; S2, pretreating coconut shells and sheep manure; S3, pretreating broken stones and wood shavings; S4, preparing a substrate of a bottom layer; S5, preparing a substrate of a surface layer; and S6, paving and forming. The survival rate of transplanting of dendrobium candidum tissue culture seedlings of the culture substrate prepared by the method is high, and can reach 86.67%; sufficient nutritional ingredients are provided for dendrobium candidum, and growth of plants and root systems of the dendrobium candidum are promoted effectively.

Description

technical field [0001] The invention belongs to the technical field of planting, and in particular relates to a preparation method of a dendrobium candidum cultivation substrate. Background technique [0002] Dendrobium officinale is a herbaceous plant of the genus Dendrobium in the family Orchidaceae. Wild Dendrobium officinale grows on limestone or trees with bryophytes at an altitude of 1600 meters. It is mainly distributed in Anhui, Zhejiang, Fujian and other places in China. Its stems are used as medicine. Dendrobium officinale is sweet in taste and slightly cold in nature. It has the functions of nourishing the stomach and promoting body fluid, nourishing yin and clearing heat, nourishing the lungs and kidneys, strengthening the waist, anti-tumor, anti-aging, enhancing immunity, and dilating blood vessels. [0003] Wild Dendrobium officinale has a unique growth environment and is very strict on the climate. It grows epiphytically on tree trunks or under forests, usual...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00C05G1/00C05F15/00
CPCA01G24/00A01G24/23C05D9/00C05F3/00C05F5/002C05F11/00
Inventor 杨良海
Owner 巫溪县琼海农业有限公司
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