Micrografting method for tetraploid acacia

A technology of tetraploid Robinia pseudoacacia and micro-grafting, which is applied in botany equipment and methods, horticulture, application, etc., can solve the problems that restrict the popularization and application of tetraploid Robinia pseudoacacia, complicated process steps, and long seedling breeding period, and achieve high feeding efficiency. Value and economic value, conducive to breeding and promotion, and the effect of short seedling cycle

Inactive Publication Date: 2013-04-24
河南省木本饲料工程技术研究中心
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  • Summary
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the current propagation methods of tetraploid Robinia pseudoacacia have defects such as complicated process steps, low survival rate, and long seedling breeding period, which restrict the popularization and application of tetraploid Robinia pseudoacacia.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A method for micro-grafting of tetraploid black locust, the method comprises the steps of:

[0018] 1) Obtain seedlings of diploid Robinia pseudoacacia seeds: Select diploid Robinia pseudoacacia seeds with full grains and no disease, soak them in 70°C water for 5 minutes, take them out, soak them in 20°C warm water for 2 days, and sow them on the ground with fine sand Germinate on the seedbed, keep the temperature of the seedbed at 25°C and the humidity at 80-85% to facilitate seed germination. After 7 days, the seeds germinate, and when the seedlings grow to the point where the true leaves are about to unfold, they can be used as seedlings for rootstocks.

[0019] 2) Use diploid black locust seedlings as rootstocks and tetraploid black locust tissue-cultured seedlings as scions, using split grafting method (specifically: take the diploid black locust seedlings out of the sand bed, and cut them from 1cm below the terminal buds) Cut the cutting knife vertically at 1.5cm ...

Embodiment 2

[0022] A method for micro-grafting of tetraploid black locust, the method comprises the steps of:

[0023] 1) Obtain seedlings of diploid Robinia pseudoacacia seeds: Select diploid Robinia pseudoacacia seeds with full granules and no disease, soak them in water at 75°C for 3 minutes, take them out, soak them in warm water at 28°C for 1 day, and sow them on the ground with fine sand Germinate on the seedbed, keep the temperature of the seedbed at 27°C and the humidity at 80-85% to facilitate seed germination. After 5 days, the seeds germinate, and when the seedlings grow to the point where the true leaves are about to unfold, they can be used as seedlings for rootstocks.

[0024] 2) Use diploid black locust seedlings as rootstocks and tetraploid black locust tissue-cultured seedlings as scions, using split grafting method (specifically: take the diploid black locust seedlings out of the sand bed, and cut them from 1cm below the terminal buds) Cut the cutting knife vertically at...

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PUM

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Abstract

The invention relates to a micrografting method for a tetraploid acacia. The method includes the steps of selecting a seedling of a diploid acacia seed as a rootstock, selecting a tissue culture seedling of the tetraploid acacia as a scion, subjecting the scion and the rootstock to cambium alignment by using a cleft grafting method, twining the interface area with silver paper slips, fixing the silver paper twining area with a grafting clamp, planting the grafted seedling into a potted tray containing matrix, then processing a routine management for 20 - 30 days, and acquiring the plant regeneration. By means of the micrografting method, an integral plant is obtained after cultivating the grafted seedling for 20 - 30 days, and accordingly the high efficiency of rapid planting is achieved. The plant regeneration cultivated by the micrografting method has the advantages of stable genetic character, healthy plant, strong growth, short seedling period, fast plant growing and high survival rate ( with a survival rate over 90% in a field planting production land).

Description

technical field [0001] The invention belongs to the technical field of plant micro-grafting methods, and in particular relates to a micro-grafting method suitable for tetraploid Robinia pseudoacacia. Background technique [0002] With the widespread planting of tetraploid Robinia pseudoacacia, its excellent characteristics such as fast growth, drought resistance, barren resistance, salt and alkali resistance, and strong fecundity have been fully utilized in my country's forestry ecological benefits, and good ecological benefits, economic benefits and Social benefits, it is the first choice for windbreak and sand-fixing forests, farmland shelter forests, barren hills greening and returning farmland to forests. The development and utilization of tetraploid black locust play a positive role in promoting the development of forestry industry. However, most of the current propagation methods of tetraploid Robinia pseudoacacia have defects such as complex process steps, low surviva...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/06
Inventor 常军刘晓明聂琳
Owner 河南省木本饲料工程技术研究中心
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