Thermo-sensitive gelatin preparation for endometrium stem cells and preparation method and application of thermo-sensitive gelatin preparation

A technology of endometrial stem cells and temperature-sensitive gel, which is applied in the field of temperature-sensitive gel preparations of endometrial stem cells and its preparation, can solve the problems of body injury, large amount of stem cell preparations, loss, etc., to promote repair, avoid multiple injuries, The effect of suppressing tissue inflammation

Pending Publication Date: 2019-12-20
GUANGDONG CHINAHEALTH LIFE SCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In recent years, stem cell preparations have been used for wound repair, but the existing stem cell preparations are all liquid. After intravenous reinfusion, the cel...

Method used

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  • Thermo-sensitive gelatin preparation for endometrium stem cells and preparation method and application of thermo-sensitive gelatin preparation
  • Thermo-sensitive gelatin preparation for endometrium stem cells and preparation method and application of thermo-sensitive gelatin preparation
  • Thermo-sensitive gelatin preparation for endometrium stem cells and preparation method and application of thermo-sensitive gelatin preparation

Examples

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Embodiment 1

[0031] This embodiment provides a method for preparing a thermosensitive gel preparation of endometrial stem cells, comprising the following steps:

[0032] (1) Menstrual collection and endometrial stem cell isolation and culture

[0033] Use Hangzhou S-Evans biosciences menstrual blood collection kit to obtain endometrial or menstrual cell samples from the menstrual cycle, and then transfer these cells to phosphate buffered saline containing penicillin / streptomycin and heparin at 4°C Save it. Send the sample to the laboratory for centrifugation within 24h to 48h after obtaining the sample, take the supernatant, conduct cell culture after bacterial inspection.

[0034] This example is the separation of menstrual blood stem cells, and the separation method uses references: Wang Xueli, Experimental Research on the Biological Characteristics and Multidirectional Differentiation Potential of Menstrual Blood-derived Endometrial Stem Cells, Master's Degree Thesis of Anhui Medical U...

Embodiment 2

[0048] The only difference between this example and Example 1 is that the density of P2-P5 endometrial stem cells is 5×10 6 cells / mL.

Embodiment 3

[0050] The only difference between this example and Example 1 is that the density of P2-P5 endometrial stem cells is 1×10 7 cells / mL.

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Abstract

The invention provides a thermo-sensitive gelatin preparation for endometrium stem cells. The thermo-sensitive gelatin preparation comprises an endometrium stem cell cellular preparation, an endometrium stem cell exosome preparation, chitosan, mannitol, hyaluronic acid, sodium alginate and beta-sodium glycerophosphate. The thermo-sensitive gelatin preparation is free from puncturing and recyclingand only needs to be transmitted into the lumina of an external genital system of women, the thermo-sensitive gelatin is cross-linked to form a semisolid preparation being in complete cooperation withthe shape of the lumina, the repair effect can be achieved, and repeated damage to organisms can be avoided. After being applied, the composite preparation can gradually dissolve through the temperature of human bodies to form a solution, mesenchymal stem cells in the preparation are released, and through polarization, cambium is obtained. Thermo-sensitive gelatincell factors in the preparation can be released slowly, and the defect of massive running off of the cells after recycling is performed can be overcome. The active factors secreted by the stem cells can restrain tissue inflammation,improve the regeneration capacity of tissue and promote repair of original tissue, and can achieve the effect of wound repair.

Description

technical field [0001] The invention relates to a medical composite biological material, in particular to a thermosensitive gel preparation of endometrial stem cells, a preparation method and application thereof. Background technique [0002] Thermosensitive gel refers to a preparation that transforms from a liquid into a non-chemically cross-linked semi-solid gel by utilizing the phase transition of the polymer material in response to the external temperature after administration in a solution state. The temperature-sensitive gel material can undergo a certain phase change with the change of the ambient temperature, has a lower critical solution temperature (LCST), and has the characteristics of controlled release, sustained release, targeting, and reduced toxicity in the human body; it can realize injection implantation Injection and long-term release, widely used in ophthalmic and nasal administration, rectal administration and injection administration. [0003] In the p...

Claims

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Application Information

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IPC IPC(8): A61K9/06A61K47/36A61K47/26A61K47/10A61K35/28A61P15/00
CPCA61K9/0034A61K9/06A61K35/28A61K47/10A61K47/26A61K47/36A61P15/00
Inventor 李文东宋云庆黎波卢瑞珊
Owner GUANGDONG CHINAHEALTH LIFE SCI CO LTD
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