Application of Arabidopsis transcription factor in breeding drought-resistant salt-tolerant rice

Abstract

The invention relates to application of Arabidopsis transcription factor in breeding drought-resistant salt-tolerant rice. Nucleotide sequences of the Arabidopsis transcription factor MYB44 are shown in SEQ ID NO.1. Encoded protein sequences of the Arabidopsis transcription factor MYB44 are shown in SEQ ID NO.2. Conditions that are met for the nucleotide sequences and the encoded protein sequences include: first, DNA (deoxyribonucleic acid) sequences shown on the 88th site to the 1005th site in the sequence table SEQ ID NO.1, or the sequences highly homologous to the DNA sequences shown on the 88th site to the 1005th site in the SEQ ID NO.1; second, other encodable sequencecs with the same protein as the DNA sequences shown in the sequence table SEQ ID NO.2; third, the sequences the same functional as the DNA sequences shown on the 88th site to the 1005th site in the SEQ ID NO.1, or sub-segments contained in the highly homologous DNA sequences shown on the 88th site to the 1005th site in the SEQ ID NO.1. The Arabidopsis transcription factor is applied to breeding of the drought-resistant salt-tolerant rice. An expression vector of the MYB44 gene can be introduced into plant cells by biotechnology. Transforming hosts, available to use the expression vector containing the MYB44 gene, can be monocotyledons such as rice, corn and wheat. The Arabidopsis transcription factor is also applicable to dicotyledons such as tobacco and soybean. The Arabidopsis transcription factor is used to breed drought-resistant salt-tolerant plant varieties.

Description

technical field

[0001] The invention belongs to the field of plant biotechnology, and in particular relates to the separation and cloning, transformation, functional verification and application of a DNA fragment (gene) of an Arabidopsis transcription factor and rice. In particular, it relates to the application and method of using the gene to enhance the stress resistance of rice such as drought resistance and salt tolerance. Background technique

[0002] Drought and salinity are two important adversity factors that affect agricultural production and ecological environment in the world today, and cause huge losses to world food production. Under the severe pressure of increasing population, decreasing arable land and insufficient fresh water resources, how to efficiently use limited fresh water resources for maximum agricultural production is one of the major issues that international and domestic bioscience and technology urgently need to solve. Rice is the most water-int...

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