13 results about "Monocotyle" patented technology

A fuel pellet is disclosed. The fuel pellet comprises a plant fiber and a lignin modifying enzyme exogenous to the plant fiber. In some embodiments, the plant fiber is a Tracheophytes plant fiber. In some embodiments, the plant fiber is a monocot plant fiber. In some embodiments, the plant fiber is a Poaceae plant fiber. In some embodiments, the plant fiber is a Saccharum plant fiber. In some embodiments, the plant fiber is sugarcane bagasse plant fiber. In some embodiments, the pellet does not contain a binding agent. In embodiments without a binding agent, the fuel pellet is held together by lignin, which is present in the plant fiber and modified to hold the plant fibers together. In some embodiments, the lignin modifying enzyme is a lignase. In some other embodiments the lignin modifying enzyme is a laccase. The disclosure further provides a method of making a fuel pellet.

Feedstock including an amphiphilic lignocellulosic biomass can be processed using enzymes prior to pulping. The enzymes break down the biomass by disrupting the surface or cell walls or by reducing wax or other components. Such enzymes include cellulases, hemicellulases, laccases, peroxidases, and pectinases. Suitable enzymes can be obtained from secretions of microbes in soil organic matter from soil in which the feedstock was grown. If the pulping process includes steam explosion, such enzy matic processing of a biomass prior to steam explosion will increase absorption of water by the processed feedstock. In steam explosion, due to the increased ability of the processed feedstock to absorb water, steam explosion is more effective in breaking apart the processed feedstock. For other pulping processes, such enzymatic treatments break down the biomass, enabling extraction of the desired components from the processed feedstock through subsequent processing using less energy and fewer chemicals.

The invention belongs to the technical field of plant promoters, and discloses an expression cassette for efficiently indicating the distribution position of auxin in wheat and application of the expression cassette. A DR5V2 promoter fragment induced by auxin IAA is constructed, a beta-glucosidase (GUS) reporter gene is started by the fragment, and the expression fragment is introduced into a monocotyledonous plant high-efficiency expression vector pBK-UBI. On the basis, a plant recombinant expression vector and recombinant bacteria containing a mini 35S promoter and a nuclear localization signal are constructed and are used for promoting expression of a reporter gene in auxin-enriched tissues of plants. After the auxin distribution indication expression box disclosed by the invention is transferred into wheat, the distribution of auxin can be observed by naked eyes through dyeing. The expression cassette can enable the reporter gene to be expressed at the wheat auxin aggregation position, and has important application value in the fields of wheat tillering and spikelet development basic theory research, wheat specific variety auxin distribution verification, wheat new material cultivation and the like.

The invention discloses an endogenous promoter AMGT1P11 of mangrove plant avicennia marina and application of the endogenous promoter AMGT1P11, and belongs to the technical field of gene engineering, and the nucleotide sequence of the endogenous promoter AMGT1P11 comprises a sequence as shown in SEQ ID NO: 1, a sequence as shown in SEQ ID NO: 2, a sequence as shown in SEQ ID NO: 3 and a sequence as shown in SEQ ID NO: 4; or a sequence which has at least 70% homology with the sequence as shown in SEQ ID NO: 1 and has the same function as the sequence as shown in SEQ ID NO: 1; or, one or more basic groups are added, substituted, inserted or deleted in the sequence shown in SEQ ID NO: 1, and the sequence has the same function as the sequence shown in SEQ ID NO: 1. The AMGT1P11 contains a reported salt-induced cis-acting element GT1-motif (GGTTAA, a new reference can be provided for research of a mangrove plant salt-tolerant mechanism, meanwhile, the promoter can regulate and control expression of exogenous target genes in dicotyledons and monocotyledons, a brand new tool and selection are provided for plant transgenic engineering, and an important foundation is laid for plant genetic improvement.

The purpose of this invention is to provide a herbicidal composition capable of expanding the herbicidal activity spectrum of a compound represented by formula [1], which is novel and has herbicidal activity, and capable of appropriately controlling monocotyledon and dicotyledon, both of which are important weeds, over a long period of time with a smaller amount of active ingredient.　The herbicidal composition contains (a) the compound represented by formula [1] or a salt thereof, and (b) at least one kind of agricultural / horticultural herbicides.

The invention discloses a flow field structure of a bionic monocotyledon vein and application of the flow field structure in an all-vanadium redox flow battery, the flow field structure comprises a rib part of the flow field structure, a flow branch, a flow field inlet, a flow field outlet and a main channel, the main channel is arranged between the flow field inlet and the flow field outlet, flow branches with a certain distance are arranged on the two sides of the main channel, and ribs of a flow field structure are arranged between the flow branches. The unique flow field structure design improves the battery performance in principle by optimizing the electrolyte flow path, has huge potential in the practical engineering application of a large-scale energy storage system, and has the core advantages of improving the electrolyte distribution uniformity, reducing concentration polarization and the like. A reliable technical scheme is provided for improving the overall performance of the battery, and the high requirements of a large-scale energy storage scene for the performance and stability of the battery can be effectively met.

Feedstock including an amphiphilic lignocellulosic biomass can be processed using enzymes prior to pulping. The enzymes break down the biomass by disrupting the surface or cell walls or by reducing wax or other components. Such enzymes include cellulases, hemicellulases, laccases, peroxidases, and pectinases. Suitable enzymes can be obtained from secretions of microbes in soil organic matter from soil in which the feedstock was grown. If the pulping process includes steam explosion, such enzy matic processing of a biomass prior to steam explosion will increase absorption of water by the processed feedstock. In steam explosion, due to the increased ability of the processed feedstock to absorb water, steam explosion is more effective in breaking apart the processed feedstock. For other pulping processes, such enzymatic treatments break down the biomass, enabling extraction of the desired components from the processed feedstock through subsequent processing using less energy and fewer chemicals.

The invention relates to a 5-acyl barbituric acid compound containing a pyrimidinedione structure, a preparation method of the 5-acyl barbituric acid compound and application of a herbicide of the 5-acyl barbituric acid compound. The phenoxyalkyl acyl chloride compound containing the pyrimidinedione structure is obtained by taking a nitrophenol compound as a raw material through nucleophilic reaction, hydrogenation reduction reaction, condensation ring closing reaction, hydrolysis reaction and acylating chlorination reaction. The 5-acyl barbituric acid compound containing the pyrimidinedione structure is obtained through an intermolecular condensation reaction with a barbituric acid compound, and a weeding test result shows that the yield of the 5-acyl barbituric acid compound containing the pyrimidinedione structure is increased at the dosage of 150 g / hectare in post-seedling treatment. The compound disclosed by the invention has a good growth inhibition effect on dicotyledonous plants such as rape, piemarker, amaranthus retroflexus and chenopodium album and monocotyledonous plants such as barnyard grass, digitaria sanguinalis, eleusine indica and green bristlegrass, and is a broad-spectrum herbicide with a novel structure and a wide application prospect.

The invention discloses a method for realizing genome large fragment inversion editing based on a guide editing system. According to the method disclosed by the invention, by virtue of an efficient multi-gene guide editing protein construct ePPEplus in a plant, paired pegRNA (pegL and pegR) is designed, and then through the processes of reverse transcription, DNA double-chain annealing, DNA chain synthesis, repairing and the like, large-fragment inversion of the plant is realized. Wherein the pegL contains RTT-L, the pegR contains RTT-R, the nucleotide sequence of the RTT-L is the same as the nucleotide sequence of the A chain at the right tail end of the DNA double chain to be inverted, and the nucleotide sequence of the RTT-R is the same as the nucleotide sequence of the B chain at the left tail end of the DNA double chain to be inverted. Tests in monocotyledon wheat, dicotyledon native tobacco and tomato show that the method disclosed by the invention is an effective method for mediating accurate inversion of the large-fragment DNA of the plant.

This invention discloses substituted isoxazoline carboxylic acid methyl ester compounds, their preparation methods, and applications. Using concentrated sulfuric acid as a solvent, concentrated nitric acid is reacted with compound (I) to generate compound (II), which then reacts with a saturated aqueous solution of hydroxylamine hydrochloride to generate compound (III). Compound (III) reacts with NCS and MMA to generate compound (IV). Iron powder is used as a reducing agent to reduce compound (IV) to generate compound (V). Using DCM and n-butanol as solvents, and EDCl and DMAP as condensing agents, compound (V) is reacted with compound (VI) to obtain the target product, the substituted isoxazoline carboxylic acid methyl ester compound. Herbicidal activity tests at 100 ppm show excellent herbicidal activity in dicotyledonous rapeseed and monocotyledonous wheat. Compound VII12, at a concentration of 10 ppm, also exhibits excellent herbicidal activity in dicotyledonous rapeseed and monocotyledonous wheat.

The invention discloses a non-embryonic stage grafting method of monocotyledonous plants and application of grafted bodies of the monocotyledonous plants, and aims to solve the technical problems that the existing monocotyledonous plant embryonic stage grafting technology is extremely short in operation window period, strict in environmental requirement and incapable of being applied in a large scale. The key technical means of the method is that a stock and a scion which are in a non-embryonic development stage (such as a one-leaf one-core stage) are selected, notches are formed in the middle stem parts of the stock and the scion and are in butt joint, and callus formation and vascular connection are promoted through specific fixing and moisturizing treatment. According to the method, the effective grafting time is remarkably prolonged, the dependence on a sterile environment is reduced, the grafting survival rate and the operation feasibility are greatly improved, and an effective means is provided for field large-scale grafting and physiological research of monocotyledonous plants.

The present invention relates to the technical field of plant genetic improvement, and in particular to the use of the TaPLT8A gene or TaPLT8A protein in regulating the iron content of monocotyledonous plant grains. The nucleotide sequence of the TaPLT8A gene of the present invention is shown in SEQ ID NO.1; the amino acid sequence of the TaPLT8A protein is shown in SEQ ID NO.2. The results of the specific embodiments of the present invention show that overexpressing the TaPLT8A gene in wheat plants can significantly increase the iron content of wheat grains; knocking out the gene in wheat significantly reduces the iron content of wheat grains. It can be seen that the TaPLT8A gene or TaPLT8A protein can regulate the iron content in wheat grains, and this study provides a usable gene resource for breeding high-iron wheat varieties.

The invention discloses a bionic monocotyledon flow field structure added with a circular flow guide block, which is applied to a vanadium redox flow battery. The circular flow guide block is arranged in the main channel, a plant vein branch structure is simulated, an electrolyte is guided to uniformly flow into a branch flow channel, the distribution of active substances is improved, concentration polarization is reduced, and the energy efficiency and stability of the battery are improved. Experiments show that compared with a traditional flow field, the energy efficiency is improved by 1.983%, the coulombic efficiency is kept about 97.6%, the problem of uneven flow of the electrolyte is effectively solved, and the method is suitable for large-scale energy storage.