6 results about "Monocotyle" patented technology

Feedstock including an amphiphilic lignocellulosic biomass can be processed using enzymes prior to pulping. The enzymes break down the biomass by disrupting the surface or cell walls or by reducing wax or other components. Such enzymes include cellulases, hemicellulases, laccases, peroxidases, and pectinases. Suitable enzymes can be obtained from secretions of microbes in soil organic matter from soil in which the feedstock was grown. If the pulping process includes steam explosion, such enzy matic processing of a biomass prior to steam explosion will increase absorption of water by the processed feedstock. In steam explosion, due to the increased ability of the processed feedstock to absorb water, steam explosion is more effective in breaking apart the processed feedstock. For other pulping processes, such enzymatic treatments break down the biomass, enabling extraction of the desired components from the processed feedstock through subsequent processing using less energy and fewer chemicals.

The invention belongs to the technical field of plant promoters, and discloses an expression cassette for efficiently indicating the distribution position of auxin in wheat and application of the expression cassette. A DR5V2 promoter fragment induced by auxin IAA is constructed, a beta-glucosidase (GUS) reporter gene is started by the fragment, and the expression fragment is introduced into a monocotyledonous plant high-efficiency expression vector pBK-UBI. On the basis, a plant recombinant expression vector and recombinant bacteria containing a mini 35S promoter and a nuclear localization signal are constructed and are used for promoting expression of a reporter gene in auxin-enriched tissues of plants. After the auxin distribution indication expression box disclosed by the invention is transferred into wheat, the distribution of auxin can be observed by naked eyes through dyeing. The expression cassette can enable the reporter gene to be expressed at the wheat auxin aggregation position, and has important application value in the fields of wheat tillering and spikelet development basic theory research, wheat specific variety auxin distribution verification, wheat new material cultivation and the like.

The invention discloses an endogenous promoter AMGT1P11 of mangrove plant avicennia marina and application of the endogenous promoter AMGT1P11, and belongs to the technical field of gene engineering, and the nucleotide sequence of the endogenous promoter AMGT1P11 comprises a sequence as shown in SEQ ID NO: 1, a sequence as shown in SEQ ID NO: 2, a sequence as shown in SEQ ID NO: 3 and a sequence as shown in SEQ ID NO: 4; or a sequence which has at least 70% homology with the sequence as shown in SEQ ID NO: 1 and has the same function as the sequence as shown in SEQ ID NO: 1; or, one or more basic groups are added, substituted, inserted or deleted in the sequence shown in SEQ ID NO: 1, and the sequence has the same function as the sequence shown in SEQ ID NO: 1. The AMGT1P11 contains a reported salt-induced cis-acting element GT1-motif (GGTTAA, a new reference can be provided for research of a mangrove plant salt-tolerant mechanism, meanwhile, the promoter can regulate and control expression of exogenous target genes in dicotyledons and monocotyledons, a brand new tool and selection are provided for plant transgenic engineering, and an important foundation is laid for plant genetic improvement.

Feedstock including an amphiphilic lignocellulosic biomass can be processed using enzymes prior to pulping. The enzymes break down the biomass by disrupting the surface or cell walls or by reducing wax or other components. Such enzymes include cellulases, hemicellulases, laccases, peroxidases, and pectinases. Suitable enzymes can be obtained from secretions of microbes in soil organic matter from soil in which the feedstock was grown. If the pulping process includes steam explosion, such enzy matic processing of a biomass prior to steam explosion will increase absorption of water by the processed feedstock. In steam explosion, due to the increased ability of the processed feedstock to absorb water, steam explosion is more effective in breaking apart the processed feedstock. For other pulping processes, such enzymatic treatments break down the biomass, enabling extraction of the desired components from the processed feedstock through subsequent processing using less energy and fewer chemicals.

This invention discloses substituted isoxazoline carboxylic acid methyl ester compounds, their preparation methods, and applications. Using concentrated sulfuric acid as a solvent, concentrated nitric acid is reacted with compound (I) to generate compound (II), which then reacts with a saturated aqueous solution of hydroxylamine hydrochloride to generate compound (III). Compound (III) reacts with NCS and MMA to generate compound (IV). Iron powder is used as a reducing agent to reduce compound (IV) to generate compound (V). Using DCM and n-butanol as solvents, and EDCl and DMAP as condensing agents, compound (V) is reacted with compound (VI) to obtain the target product, the substituted isoxazoline carboxylic acid methyl ester compound. Herbicidal activity tests at 100 ppm show excellent herbicidal activity in dicotyledonous rapeseed and monocotyledonous wheat. Compound VII12, at a concentration of 10 ppm, also exhibits excellent herbicidal activity in dicotyledonous rapeseed and monocotyledonous wheat.

The invention discloses a non-embryonic stage grafting method of monocotyledonous plants and application of grafted bodies of the monocotyledonous plants, and aims to solve the technical problems that the existing monocotyledonous plant embryonic stage grafting technology is extremely short in operation window period, strict in environmental requirement and incapable of being applied in a large scale. The key technical means of the method is that a stock and a scion which are in a non-embryonic development stage (such as a one-leaf one-core stage) are selected, notches are formed in the middle stem parts of the stock and the scion and are in butt joint, and callus formation and vascular connection are promoted through specific fixing and moisturizing treatment. According to the method, the effective grafting time is remarkably prolonged, the dependence on a sterile environment is reduced, the grafting survival rate and the operation feasibility are greatly improved, and an effective means is provided for field large-scale grafting and physiological research of monocotyledonous plants.