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47 results about "Monocotyle" patented technology

Application of Arabidopsis transcription factor in breeding drought-resistant salt-tolerant rice

The invention relates to application of Arabidopsis transcription factor in breeding drought-resistant salt-tolerant rice. Nucleotide sequences of the Arabidopsis transcription factor MYB44 are shown in SEQ ID NO.1. Encoded protein sequences of the Arabidopsis transcription factor MYB44 are shown in SEQ ID NO.2. Conditions that are met for the nucleotide sequences and the encoded protein sequences include: first, DNA (deoxyribonucleic acid) sequences shown on the 88th site to the 1005th site in the sequence table SEQ ID NO.1, or the sequences highly homologous to the DNA sequences shown on the 88th site to the 1005th site in the SEQ ID NO.1; second, other encodable sequencecs with the same protein as the DNA sequences shown in the sequence table SEQ ID NO.2; third, the sequences the same functional as the DNA sequences shown on the 88th site to the 1005th site in the SEQ ID NO.1, or sub-segments contained in the highly homologous DNA sequences shown on the 88th site to the 1005th site in the SEQ ID NO.1. The Arabidopsis transcription factor is applied to breeding of the drought-resistant salt-tolerant rice. An expression vector of the MYB44 gene can be introduced into plant cells by biotechnology. Transforming hosts, available to use the expression vector containing the MYB44 gene, can be monocotyledons such as rice, corn and wheat. The Arabidopsis transcription factor is also applicable to dicotyledons such as tobacco and soybean. The Arabidopsis transcription factor is used to breed drought-resistant salt-tolerant plant varieties.
Owner:UNIV OF SCI & TECH OF CHINA

Isolated nucleic acid molecules from the genome of citrus leprosis virus and uses thereof

The present invention relates to nucleic acid molecules found in the genome of the Citrus Leprosis Virus (CiLV), which is associated to Citrus Leprosis (CiL) disease. The cloned CiLV nucleic acid molecules can be used as probes or can be used to design oligonucleotide primers useful in assays, such as a polymerase chain reaction, for detecting the presence of CiLV in biological samples, particularly leaves, roots and other tissues or organs of plants, such as plants from the genera Citrus and Poncirus. The invention comprises introducing the mentioned nucleic acid molecules in cloning vectors and cloning the recombinant nucleic acid molecules in cells, such as prokaryotes (e.g., bacteria like E. coli), and eukaryotes (e.g., yeast, COS, CHO, and other cells). The cloned CiLV nucleic acid molecules are expressed in cells to provide immunogenic proteins which can be used to raise antibodies against the CiLV, which can then be used to detect the presence of the CiLV virus in biological samples. It also comprises the nucleic acid molecules represented in SEQ ID Nos. 5 and 8, in whole or part, as well as transgenic plants, such as monocots and dicots, containing the CiLV nucleic acid molecules, in any kind of combination, so that expression increases resistance to CiL disease.
Owner:FISCHER AGROINDA

A Method for Extracting Rice Leaf Plasma Membrane Phosphorylated Proteins Suitable for Two-dimensional Electrophoresis

The invention belongs to the technical field of proteomics, specifically discloses for the first time a method for extracting rice leaf plasma membrane phosphorylated proteins suitable for two-dimensional electrophoresis, including extraction of rice leaf rough plasma membranes, purification of rice leaf plasma membranes, and rice leaf plasma membrane extraction methods. Enrichment of membrane phosphorylated proteins and other steps. The extraction method of the present invention is simple in operation, high in extraction efficiency and low in cost, the purity of the obtained rice leaf plasma membrane can reach more than 93%, and the yield of plasma membrane phosphorylated protein enriched from plasma membrane protein is 3.33-3.89% . This method is suitable for plant materials that are difficult to extract plasma membrane phosphorylated proteins and have many interfering impurities; at the same time, the obtained plasma membrane phosphorylated proteins have fewer impurities and interfering substances, which can meet the requirements of two-dimensional electrophoresis. Uniform distribution, less longitudinal tailing and horizontal stripes, and good repeatability; this method is suitable for the preparation and analysis of monocot plasma membrane phosphorylated proteomes.
Owner:SOUTH CHINA AGRI UNIV
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