Inducible promoter containing W box as well as construction method and application to genetic engineering

Abstract

The invention provides an inducible promoter containing a W box as well as a construction method and the application to genetic engineering. The inducible promoter at least contains a nucleotide sequence shown as SEQ ID No.1. The construction method comprises the following step of: fusing a stress-response-stressed W box identified in dicotyledon arabidopsis thaliana with a CaMV35S promoter core sequence to construct an inducible promoter. The inducible promoter containing the W box in the invention can strongly response the invasion of rice blast fungus and the feeding of rice cnaphalocrocis medinalis in transgenic rice, has obvious response activity on the treatment of small-molecule compounds with disease resistance after separation identification and has poor response activity on abiotic stress treatment, such as drought, low temperature, high temperature, and the like. The inducible promoter has very important application values in the disease-resistant and pest-resistant genetic engineering of monocotyledon and dicotyledon, and transgenic plant strains also have very important application values in the high throughput screening of small-molecule compounds for inducing plants to resist diseases.

Description

technical field [0001] The present invention relates to an inducible promoter and its construction and application in genetic engineering, and more specifically relates to the application of an inducible promoter containing a W box in the genetic engineering of resistance to disease and insect pests in rice monocotyledonous plants. Technical field of plant genetic engineering. Background technique [0002] The infection of pathogenic microorganisms such as fungi, bacteria, and viruses often causes a decline in the yield and quality of various crops. Breeding and promoting disease-resistant varieties is the safest and most effective way to prevent and control crop diseases and ensure stable and high yields of crops. Using the plant's own disease-resistant genes, many new disease-resistant varieties have been bred through conventional breeding and molecular marker-assisted breeding methods. However, the utilization of resistance genes among species has certain limitations. ...

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Problems solved by technology

Constitutive promoters can be used to evaluate the biological function of a gene, the amount of transgene expression and its activity, but the main problems in the practical application of improving transgenic crops are: (1) the genes driven by constitutive promoters in various plant tissues It is expressed to varying degrees in the plant, which drives the expression of exogenous genes in various tissues and all developmental stages of the plant, and produces a large number of heterologous proteins or metabolites that accumulate in the plant, breaking the original metabolic balance of the plant and increasing the growth rate of the plant. Some products are even toxic to the growth and development of plants, causing a huge waste of material and energy, and at the same time changing the shape of plants, affecting the normal growth and development of plants, and even leading to death; (2) All cells of plants may All will enter the defense mode, even when there is no pathogen infection, the disease resistance response is still carried out; (3) the transgene product regulated by the constitutive promoter can accumulate in the plant tissues eaten by humans and animals, but the biosafety requirements cannot have transgenic product

However, so far there is still lack of direct evidence that elements such as the W box respond to pathogens or elicitors in rice and monocots.

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