Application of rice spliceosomes GS3.2 in regulation and control of rice grain traits

Abstract

The invention discloses application of rice spliceosomes GS3.2 in regulation and control of rice grain traits. The invention identifies that a GS3 gene has a plurality of spliceosomes GS3.1-GS3.6, wherein the main spliceosomes are GS3.1 and GS3.2, which respectively account for about 50% and 40% of a total transcript, while GS3.2 reduces the level of GS3.1 form at the RNA level on the one hand, and competitively inhibits signal transduction of GS3.1 at the protein level on the other hand, thereby inhibiting the negative regulation and control effect of GS3.1 and achieving the effect of maintaining the normal size of rice grains. On that basis, the invention inhibits the negative grain size regulation and control function of GS3.1 by expressing GS3.2 in a GS3.1 high-expression strain, thereby achieving the purpose of maintaining the normal size of rice grains. The invention not only solves the problem that the functional differentiation mechanism of the dicotyledon AGG3 and the monocotyledon GS3 is still unclear, but also can be applied to the fields of actual production, breeding and the like of rice as a new rice grain trait regulation and control means.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to the application of a rice splicing body GS3.2 in regulating rice grain traits. Background technique [0002] Rice (Oryza sativa L.) is the most important food crop in my country and one of the most important food crops in the world. Grain size is an important determinant of rice yield. [0003] So far, scientific research has identified G protein signaling, ubiquitin-proteasome pathway, hormone signaling, and MAPK signaling pathways to regulate rice grain size, and the research and application of their mechanisms of action are mainly focused on gene transcription and post-translation levels, while transcription The post-level regulation method has not been reported and applied yet. [0004] The G protein signaling pathway regulates grain size by forming a heterotrimer of Gα, Gβ, and Gγ. In rice and wheat, the Gγ subunit GS3 negatively regulates grain size, b...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide an application of rice spliceosome GS3.2 in regulating rice grain traits, aiming to solve the problem that the functional differentiation mechanism of GS3 in dicotyledonous plants AGG3 and monocotyledonous plants is still unclear, and can be used as a A new method of regulating rice grain traits is applied to the actual production and breeding of rice

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