Chlorine resistant strain V430 and its screening method

A screening method and strain technology, applied in chemical instruments and methods, bacteria, water/sewage treatment, etc., can solve the problems of high organic matter, high chloride ion concentration, weak research, etc., and achieve the effect of efficient degradation

Inactive Publication Date: 2007-01-24
CHINA UNIV OF GEOSCIENCES (WUHAN)
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Problems solved by technology

On the one hand, this type of wastewater has complex components, many refractory organic substances, and poor biodegradability of the wastewater; on the other hand, the wastewater has a high salt content and a high concentration of chloride ions, and the concentration of chloride ions in the mixed wastewater can reach 20,000 mg/L
At present, non-biological methods and biological methods are used for salt-containing organic wastewater. The non-biological method is unacceptable to enterprises due to its high treatment cost and poor treatment effect. The biochemical method has always been the first meth...

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  • Chlorine resistant strain V430 and its screening method
  • Chlorine resistant strain V430 and its screening method
  • Chlorine resistant strain V430 and its screening method

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Embodiment Construction

[0034] 1. Screening method for a chlorine-resistant bacterial strain V430:

[0035] (1) Material preparation:

[0036] 1. Bacterial source and experimental wastewater:

[0037] (1) Bacterial source: activated sludge in the biochemical system for treating saponin wastewater;

[0038] (2) Experimental wastewater (i.e. mixed saponin wastewater): The experimental wastewater is taken from the saponin production wastewater of Fangkun Real Estate Co., Ltd., Shiyan City, Hubei Province; after internal electrolysis and CaO deployment, the specific indicators are: pH=7.5-8.2, COD=4000-17000mg / L, Cl - =8000-30000mg / L, NH 4 -N 140-300mg / L.

[0039] 2. Culture medium:

[0040] Separation (complete) medium: peptone 10g; beef extract 5g; sodium chloride; 5g; distilled water 1000ml.

[0041] Screening medium: MgSO 4 ·7H 2 O, 0.2g / L, KH 2 PO 4 0.5g / L, K 2 HPO 4 1.5g / L, NH 4 Cl 0.5 / L, CaCl 2 135g / L, 1ml trace element solution, dissolved in 1L saponin wastewater (CO...

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Abstract

The present invention relates to a kind of chlorine tolerant microbe and its screening process. The chlorine tolerant microbe strain is Staphylococcus saprophyticus V430 in the preservation number of CCTCC No. M205145. The Staphylococcus saprophyticus V430 has circular opaque colony shape with lug and smooth edges; spherical single, paired or piled cells of d in 0.6-0.9 micron without flagellum; no motion, no endogeneous spore, gram positive and anaerobic property; and optimal growth pH value of 6.0-7.5 and temperature of 25-45 deg.c. It can survive in high chlorine ion concentration environment and degrade high concentration organic pollutant effectively.

Description

technical field [0001] The invention relates to a chlorine-resistant microorganism and a screening method thereof. Background technique [0002] High-salinity refractory organic wastewater, such as petroleum exploration, chemical, pharmaceutical and other wastewater, has become a worldwide technical problem in the field of environmental protection water treatment. However, the hydrochloric acid method turmeric saponin production wastewater is representative of the high-concentration saline refractory organic wastewater. On the one hand, this type of wastewater has complex components, many refractory organic substances, and poor biodegradability of the wastewater; on the other hand, the wastewater has a high salt content and a high concentration of chloride ions, and the concentration of chloride ions in the mixed wastewater can reach 20,000 mg / L. At present, non-biological methods and biological methods are used for saline organic wastewater. The non-biological method is un...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F1/58
Inventor 信欣王焰新鲍建国刘慧杨雪芬李平
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)
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