Benzene series compound facultative anaerobic degrading bacterium
A benzene compound, facultative anaerobic technology, applied in the directions of bacteria, microorganisms, water pollutants, etc., can solve the problems of low production capacity of degradation reaction, incomplete anaerobic degradation mechanism, and difficult to complete degradation, and achieve environmental adaptation. strong effect
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[0015] 1 Screening method of a facultative anaerobic degrading bacteria for benzene series compounds
[0016] 1.1 Material preparation
[0017] 1.1.1 Bacteria source
[0018] Soil samples 5-10 cm below the surface were collected in the oil-polluted area of Daqing Oilfield.
[0019] 1.1.2 Culture medium
[0020] Inorganic salt separation medium: 0.42g·L -1 NaNO 3 , 0.27g·L -1 NH 4 Cl, 7.9g·L -1 Na 2 HPO 4 ·12H 2 O, 1.5g·L -1 KH 2 PO 4 , 1.0g·L -1 MgSO 4 ·7H 2 O, 1.0mL·L -1 Trace element solution (the ratio of trace element solution is: 2.14g·L -1 ZnCl 2 , 2.5g·L -1 MnCl 2 ·4H 2 O, 0.3g·L -1 CoCl 2 ·6H 2 O, 0.2g·L -1 CuCl 2 ·2H 2 O, 0.4g·L -1 NaMoO 4 ·2H 2 O, 4.5g·L -1 CaCl 2 ·2H 2 O, 2.9g·L -1 FeCl 3 ·6H 2 O, 1.0g·L -1 H 3 BO 3 , 0.1g·L -1 KI, 1L deionized water), 0.472g·L -1 Sodium succinate, 1 L distilled water, pH=7.0.
[0021] Agar medium: 1L inorganic salt separation medium + 20g·L -1 agar.
[0022] LB medium: 1.0g·L -1 Yeast ...
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