Benzene series compound facultative anaerobic degrading bacterium

A benzene compound, facultative anaerobic technology, applied in the directions of bacteria, microorganisms, water pollutants, etc., can solve the problems of low production capacity of degradation reaction, incomplete anaerobic degradation mechanism, and difficult to complete degradation, and achieve environmental adaptation. strong effect

Inactive Publication Date: 2010-11-24
CHINA UNIV OF GEOSCIENCES (WUHAN)
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, the rapid consumption of dissolved oxygen in groundwater creates a large number of anaerobic zones for microorganisms. Therefore, many studies have focused on the anaerobic biodegradation of benzene series compounds in the past two decades, but the mechanism of anaerobic degradation is still unclear. fully aware
At the same time, the biodegradation of anaerobic benzene series compounds faces problems such as low degradation reaction capacity and difficult to complete degradation.

Method used

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  • Benzene series compound facultative anaerobic degrading bacterium
  • Benzene series compound facultative anaerobic degrading bacterium
  • Benzene series compound facultative anaerobic degrading bacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0015] 1 Screening method of a facultative anaerobic degrading bacteria for benzene series compounds

[0016] 1.1 Material preparation

[0017] 1.1.1 Bacteria source

[0018] Soil samples 5-10 cm below the surface were collected in the oil-polluted area of ​​Daqing Oilfield.

[0019] 1.1.2 Culture medium

[0020] Inorganic salt separation medium: 0.42g·L -1 NaNO 3 , 0.27g·L -1 NH 4 Cl, 7.9g·L -1 Na 2 HPO 4 ·12H 2 O, 1.5g·L -1 KH 2 PO 4 , 1.0g·L -1 MgSO 4 ·7H 2 O, 1.0mL·L -1 Trace element solution (the ratio of trace element solution is: 2.14g·L -1 ZnCl 2 , 2.5g·L -1 MnCl 2 ·4H 2 O, 0.3g·L -1 CoCl 2 ·6H 2 O, 0.2g·L -1 CuCl 2 ·2H 2 O, 0.4g·L -1 NaMoO 4 ·2H 2 O, 4.5g·L -1 CaCl 2 ·2H 2 O, 2.9g·L -1 FeCl 3 ·6H 2 O, 1.0g·L -1 H 3 BO 3 , 0.1g·L -1 KI, 1L deionized water), 0.472g·L -1 Sodium succinate, 1 L distilled water, pH=7.0.

[0021] Agar medium: 1L inorganic salt separation medium + 20g·L -1 agar.

[0022] LB medium: 1.0g·L -1 Yeast ...

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Abstract

The invention belongs to the technical field of degrading microorganisms and relates to a benzene series compound facultative anaerobic degrading bacterium which is characterized in that the invention is microbacterium schleiferi HBSD-C CCTCC No. M209284 and is preserved in China Center for Type Culture Collection at November 27, 2009, and the preservation number is CCTCC No. M209284. The invention has the colony morphology that the benzene series compound facultative anaerobic degrading bacteria is pale yellow, circular, semi-transparent and sunken and has tidy edge and smooth surface; a cell is in a shape of a straight bar and is gram-positive and immobile; and the invention has the main biochemical characteristics that the benzene series compound facultative anaerobic degrading bacterium is oxidase-positive, catalass-positive, nitrate reduction positive, gelatin hydrolysis negative and facultative anaerobic, utilizes glucose, fructose and sucrose without utilizing maltose and grows under the temperature of 4-41 DEG C. The invention can degrade benzene, toluene, xylene and mesitylene under the anaerobic condition and has the degradation rate of 25.9-41.2 percent on benzene series compound 3d with the concentration of 175.8mg/L.

Description

technical field [0001] The invention belongs to the technical field of degrading microorganisms and the technical field of biological treatment of environmental pollutants, and particularly relates to a facultative anaerobic degrading bacteria of benzene series compounds. Background technique [0002] Benzene-based compounds are a class of volatile aromatic hydrocarbon compounds, including benzene, toluene, ethylbenzene, o-, m-, p-xylene, etc. In addition to being present in gasoline fuels, benzene-based compounds are also widely used as raw materials in rubber, Manufacturing of lubricants, dyes, detergents, pharmaceuticals and pesticides (Romy Chakraborty, John D.Coates.2005.Hydroxylationand Carboxylation-Two Crucial Steps of Anaerobic Benzene Degradation by DechloromonasStrain RCB.Applied and environmental microbiology, Sept.2005, p .5427-5432). The content of benzene series compounds in petroleum hydrocarbons is not high, but they are very important. They are the most wa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34A62D3/02C12R1/01C02F101/36C02F103/06A62D101/20
Inventor 周建伟吕敦玉余楚周爱国蔡鹤生李萍童蕾
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)
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