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Kit of ready-to-use salmonella identification plate, preparation method and use method

A Salmonella and kit technology, applied in biochemical equipment and methods, microbiological measurement/inspection, and resistance to vector-borne diseases, etc., can solve the problems of procrastination in reporting time, complicated identification steps, high cost, etc., and achieve shortened identification Effects of reporting time, greater grasp and acceptance, and reduced random error

Inactive Publication Date: 2011-04-27
SHANGHAI MUNICIPAL CENT FOR DISEASE CONTROL & PREVENTION
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It mainly solves the technical problems such as the complicated and high cost of the existing Salmonella identification steps, the bias of serum identification results caused by antigen induction or inconsistency of culture materials, and the delay in reporting time.

Method used

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  • Kit of ready-to-use salmonella identification plate, preparation method and use method
  • Kit of ready-to-use salmonella identification plate, preparation method and use method
  • Kit of ready-to-use salmonella identification plate, preparation method and use method

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Embodiment Construction

[0011] Refer to attached figure 1 , a kit for ready-to-use Salmonella identification plates, including:

[0012] Salmonella-specific enzyme-substrate screening medium 2: Ingredients: agar 17.0g, tryptone 10.0g, peptone 10.0g, yeast extract 3.0g, sodium chloride 5.0g, 5-bromo-4-chloro- 250mg of 3-indole-β-D-galactopyranoside, 250mg of octanoate, 0.5g of sulfapyridine, 1000ml of distilled water. Preparation of Salmonella-specific enzyme-substrate screening medium: Take 17.0 grams of agar powder, 10.0 g of tryptone casein, 10.0 g of peptone, 3.0 g of yeast powder, and 5.0 g of sodium chloride, heat, dissolve and boil twice in 990 ml of distilled water Finally, weigh 250 mg of 5-bromo-4-chloro-3-indole-β-D-galactopyranoside and dissolve it in 4 ml Tri.HCl (pH8.0), weigh 250 mg octanoate and dissolve it in 4 ml distilled water, Then weigh 0.5 g of sulfapyridine and dissolve it in 2 ml of 0.01 molar concentration NaOH. After the heated medium is cooled to 50°C, add it and mix well...

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Abstract

The invention relates to a method for synchronously identifying a specificity enzyme-substrate phenotype and a serum subtype of salmonella in intestinal pathogenic bacteria, which mainly solves the technical problems of the traditional salmonella identification method has complicated identification steps, higher cost, inaccurate serum identification result caused by nonuniform antigen induction or culturing materials, delayed report time, and the like. The kit of a ready-to-use salmonella identification plate comprises: (1) a salmonella specificity enzyme-substrate screening culture medium, (2) a selective culture medium aiming at the salmonella specificity O-phase antigen growth, and (3) a selective culture medium aiming at the salmonella specificity H-phase antigen growth. In the invention, the typical colonial morphology is generated by utilizing an enzyme-reaction substrate, and the salmonella O antigen and H antigen are simultaneously cultured, therefore, the method is beneficial to the serotype identification of the salmonella.

Description

technical field [0001] The present invention relates to an identification method for the specific enzyme-substrate phenotype and serotyping of Salmonella among intestinal pathogenic bacteria, in particular to the typical colony morphology produced by the enzyme-reaction substrate and the simultaneous detection of Salmonella "O"" H” antigen culture to facilitate the identification of Salmonella serotype combined kit and method of use. Background technique [0002] The current methods for identification of Salmonella biochemical phenotypes and serum phenotypes are cumbersome, with low sensitivity and specificity, and the cost of automated biochemical reaction reagents for identification of suspected colonies is expensive. Chinese patent application 200810047994.7 discloses "a method for rapid detection of Salmonella and Shigella at the same time", which belongs to the rapid detection technology of food-borne pathogens, specifically a method for simultaneous detection of Salmon...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
CPCY02A50/30
Inventor 许学斌
Owner SHANGHAI MUNICIPAL CENT FOR DISEASE CONTROL & PREVENTION
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